Ahsanur RAHMAN, Most. Ferdousi BEGUM, Matiur RAHMAN, M. A. BARI, M. Firoz ALAM, G. N. M. ILIAS

Isolation and identiication of Trichoderma species from diferent habitats and their use for bioconversion of solid waste

Farklı habitatlardan toplanan Trichoderma spp.’nin yerli populasyonlar karakterize edilmiş ve miktarları belirlenmiştir. Tanımlanan suşlar, T. harzianum (IMI-392432, 392433, 392434); T. pseudokoningii (IMI-392431) ve T. virens (IMI-392430) olarak isimlendirilmiştir. Beş suş arasından, T. harzianum bütün habitatlarda en yaygın olanıdır. Trichoderma türlerinin koloni oluşturan birimleri (cfu’ler) farklı habitatlarda önemli bir şekilde (P = 0,05) değişmiş ve olumlu bir şekilde habitatın fiziko-kimyasal özellikleri ile ilişkilendirilmiştir. Trichoderma’nın asidik bir habitata adapte olduğu bulunmuştur. Etkili biyolojik dönüşüm ajanı denenerek mutfak atığı ayrıştırmasında en etkili türün T. har izanum olduğu bulunmuştur. Spor süspansiyonu ile muamele edilen atıklardaki en yüksek hacim (% 31,80) ve ağırlık kayıpları (% 30,80) bulunmuştur. Gelecek vaadeden sonuçlar, kontrolden %18 daha fazla ayrıştırma ile sonuçlanan Trichoderma’nın farklı suş ve türleri ile kombine edilmiş bir uygulama kullanılarak da bildirilmiştir.

Farklı habitatlardan Trichoderma türlerinin izolasyonu ve tanımlanması ve katı atıkların biyolojik dönüşümü için kullanımı

The native population of Trichoderma species collected from different habitats was quantified and characterised. The identified strains are T. harzianum (IMI-392432, 392433, 392434); T. pseudokoningii (IMI-392431) and T. virens (IMI-392430). Out of five strains, T. harzianum was the most common in all of the habitats. Colony forming units (cfu’s) of Trichoderma species varied significantly (P = 0.05) in different habitats and were positively correlated with the physico- chemical characteristics of the habitat. Trichoderma was found to have adapted to an acidic habitat. In testing for an efficient bioconversion agent, T. harzianum (IMI-392432) was found to be the most effective in kitchen waste decomposition. It provided the highest volume (31.80%) and weight (30.80%) losses in waste treated with spore suspension. Promising results were also noted using a combined treatment with different strains/species of Trichoderma, which resulted in 18% greater decomposition of waste than the control.

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