Ahsanur RAHMAN, Most. Ferdousi BEGUM, Matiur RAHMAN, M. A. BARI

Isolation and identification of Trichoderma species from different habitats and their use for bioconversion of solid waste

The native population of Trichoderma species collected from different habitats was quantified and characterised. The identified strains are T. harzianum (IMI-392432, 392433, 392434); T. pseudokoningii (IMI-392431) and T. virens (IMI-392430). Out of five strains, T. harzianum was the most common in all of the habitats. Colony forming units (cfu's) of Trichoderma species varied significantly (P = 0.05) in different habitats and were positively correlated with the physico-chemical characteristics of the habitat. Trichoderma was found to have adapted to an acidic habitat. In testing for an efficient bioconversion agent, T. harzianum (IMI-392432) was found to be the most effective in kitchen waste decomposition. It provided the highest volume (31.80%) and weight (30.80%) losses in waste treated with spore suspension. Promising results were also noted using a combined treatment with different strains/species of Trichoderma, which resulted in 18% greater decomposition of waste than the control.

Anahtar Kelimeler:

Key words: Isolation, distribution, Trichoderma, kitchen waste, bioconversion

Isolation and identification of Trichoderma species from different habitats and their use for bioconversion of solid waste

Keywords:

Key words: Isolation, distribution, Trichoderma, kitchen waste, bioconversion,

PDF

___

1. Alexander M. Introduction to soil microbiology. In. Wiley J & Sons. New York and London; 1961.
2. Elad Y. Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Prot 19: 709-714, 2000.
3. Ilias GNM, Rahman MA, Molla AH et al. Composting of municipal garbage by using Trichoderma- a new approach in context of Bangladesh. Bangladesh J Genet Biotech 6 (1&2): 75- 78, 2005.
4. Bartone C. Strategies for improving municipal solid waste management: Lesson forum world bank lending and CWG activities. Workshop planning for sustainable and integrated solid waste management Manila: Washington, DC. Urban Management Division. World Bank. USA; 2000.
5. Molla AH, Razi FA, Aziz AS et al. A potential resource for bioconversion of domestic waste water sludge. Biores Technol 85: 263-272, 2002.
6. Leahy JG, Colwell RR. Microbial degradation of hydrocarbons in the environment. Microbiol Rev 54(3): 305-315, 1990.
7. Rifai MA. A revision of the genus Trichoderma. Mycological papers no. 116. Commonwealth Mycological Institute, Kew, Surrey, England. 1969.
8. AWPA. Book of Standards. American Wood-preservers Association Standards. Maryland 21666. USA; 1986.
9. Black GR. Bulk Density. In: Black CA. Ed. Methods of Soil Analysis Part 1. American Society of Agronomy. Madison, USA; 1965: Agronomy 9.
10. Page AL, Miller RH, Keeney (eds.). Methods of soil analysis. Part 2. Chemical and microbiological properties. 2nd ed. Agronomy series 9, ASA, SSSA, Madison, Wis, USA; 1982.
11. Ressel, DF. MSTAT-C. Package Program. Dept. of Crop and Soil Sci., Michigan State Univ., USA; 1996.
12. Zakaria MH. Some Aspects of the biology and chemically assisted biological control of Ganoderma species in Malaysia, PhD, University of Putra, Malaysia, 1989.
13. Ilias GNM. Trichoderma and its efficacy as a bio-control agent of basal stem rot of oil palms, PhD, University of Putra, Malaysia, 1999.
14. Okoth SA, Roimen H, Mutsotso B et al. Land use systems and distribution of Trichoderma species in Embu region, Kenya. Trop and Sub trop Agroeco Sys 7: 105-112, 2007.
15. Roiger DJ, Jeffers SN, Caldewell RW. Occurrence of Trichoderma species in apple orchard and woodland soils. Soil Biol Biochem 23: 353-359, 1991.
16. Widden P, Abitbol JJ. Seasonality of Trichoderma species in a spruce forest soil. Mycologia 72: 775-784, 1980.
17. Danielson RM, Devey CB. The abundance of Trichoderma propagules and the distribution of species in forest soil. Soil Biol Biochem 5: 485-494, 1973.
18. Eastburn DM, Butler EE. Microhabitat characterization of Trichoderma harzianum in natural soil: evaluation of factors affecting population density. Soil Biol Biochem 20: 541-545, 1988.
19. Weindling R, Fawcett HS. Experiments in biological control of Rhizoctonia damping off. Phytopathology 24: 1142, 1934. 20. Widden P. Fungal populations from forest soils in southern Quebec. Can J Bot 57: 1324-1331, 1979.
21. Roiger DJ, Jeffers SN. Evaluation of Trichoderma spp. for biological control of Phytophthora crown and root rot of apple seedlings. Phytopathology 81(8): 910-917, 1991.
22. Novak RO, Whittaingham WF. Soil and litter microfungi of a maple-elm-ash flood plain community. Mycologia 60: 776-787, 1968.
23. Raimbault M. General and microbiological aspects of solid substrate fermentation. J Biotechnol 1(3): 1-15, 1998.
24. Zheng Z, Shetty K. Cranberry processing waste for solid state fungal inoculant production. Process Biochem 33(3): 323-329, 1998.
25. Martin SB, Dale JL. Biodegradation of turf thatch with wooddecay fungi. Phytopathology 70: 297-301, 1980.
26. Bari MA, Begum MF, Sarker KK et al. Mode of action of Trichoderma spp. on organic solid waste for bioconversion. Plant Environ Dev 1(1): 61-66, 2007.
27. Hajra JN. Enrichment of compost with microbial inoculants. In: Sen SP and Palit P. ed. Biofertilizers Potentialities and Problems; Plant physiology forum, Calcutta: pp.249-254, 1988.