Işgın (Rheum ribes L.) bitkisindeki A, E ve C vitaminleri ile selenyum düzeylerinin araştırılması

Özet: Elazığ ve Tunceli yöresinde farklı rakımlarda toplanan taze ışgın (Rheum ribes L) sürgünlerinde ki A, E ve C vitaminleri ile selenyum düzeyleri araştırılmıştır. Vitamin düzeyleri High Performance Li quid Chromatography (HPLC) ile, selenyum miktarı ise florimetre ile tespit edilmiştir. Elde ettiğimiz sonuçlara göre ışgın sürgünlerindeki A, E ve C vitaminleri ile selenyum düzeylerinin sı rasıyla 0,255 ± 0,019 – 0,363 ± 0,015 µg . gr -1, 0,614 ± 0,014 – 0,765 ± 0,021 µg.gr -1, 197,6 ± 10,47 – 282,3 ± 17,46 µg. gr -1, 98,6 ± 6,88 – 141 ± 10,01 µg . kg-1 arasında değiştiği belirlen- miştir. Ayrıca yüksek rakımlı ortamlarda yaşayan bitkilerdeki antioksidan vitamin düzeylerinin düşük rakımlı ortamlarda yaşayanlara göre daha fazla olduğu görülmüştür. Bitkinin bu şekilde strese bağlı ola rak artış gösteren serbest radikallere karşı antioksidan vitamin düzeyini arttırarak mukavemet kazan dığı sonucuna varılmıştır. Materyaldeki Se içeriği ise beslenme açısından yeterli düzeydedir.

A study of levels of vitamins A, E and C and selenium in rhubarb (Rheum ribes L.)

The levels of vitamins A, E and C and selenium in young shoots of Rheum ribes L. collec-ted at various altitudes from Elazığ and Tunceli Basins were examined experimentally. The vitamin le-vels and the amount of selenium were determined by HPLC and fluorometers. It was determined that the levels of A, E and C vitamins and selenium in the young shoots of Rheum ribes L. were in the ranges of 0.255 ± 0.019 - 0.363 ± 0.015 µg.gr -1 , 0.614 ± 0.014 - 0.765 ± 0.021 µg.gr -1 , 197.6 ± 10.47 - 282.3 ± 17.46 µg .gr -1 , 98.6 ± 6.88 - 141 ± 10.01 µg . kg -1 res-pectively. It was found that the amount of antioxidant vitamins in plants growing at high altitudes was higher than that in plants growing at low altitudes. It was concluded that the plants increased their re-sistivity by increasing their antioxidant vitamin levels in opposition to the free radicals increasing with stress. The amount of selenium in the materials was found to be sufficient in terms of nutrition.

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  • 1. Davis, P.H., Flora of Turkey and The East Aegean Islands. Vol. 2. Edinburg, 1966. Edinburgh University Press, 268. 2. Baytop, T., Türkiyede Bitkiler ile Tedavi İstanbul, 1984, İstanbul Üniversitesi Yayınları No 3255 - Eczacılık Fakültesi No 40, 358. 3. Murray, R.K., Mayes, P.A., Granner, D.K., Rodwell, V.W., Harper'in Biyokimyası, İstanbul, 1993, Barış Kitapevi, 704-713. 4. Cheesman, K.H., Slater, T.F., Indroduction to Free Radical Biochemistry, Br. Med. Bull., 49 (3), 481-493. 1993. 5. Longenstroer, P., Pieper, G.M., Regulation of Spontaneous EDRF Rebase In diabetic Rat Aorta by Oxygen Free Ra- dical, Am. J. Physiol., 263:257-265, 1992. 6. Fridovic, I., Freeman Bruce, Antioxidant Defense in the Lung. Ann. Rev. Physiol. Vol.48, 693-702, 1986. 7. Dormandy, T.L., Free Radicals Oxidation and Antioxidants. The Lancet. March 25, 647-650, 1978. 8. Wilson, R.L., Peroxy Free Radicals and Enzyme Inactivation in Radiation Injury and Oxygen Toxicity: Protection by Superoxide Dismutase and Antioxidants. The Lancet. April 7; 10:804, 1984. 9. Diplock, A.T., Antioxidant Nutrients and Disease Prevention: An. Overview. Am. J. Chim Nutr., 53: 1895-1935, 1991. 10. Condell, A.R., Tappel, A.L., Evidence for Suitability of GSH-Px as a Protective Enzyme: Studies of Oxidative Dama- ge, Renaturation, and Proteolysis. Archives of Biochemistry and Biophysics. Vol.223:2, 407-416, 1983. 11. Zintzen, H., A Summary of the Vitamin E/Selenium Problem in Ruminants. News and Reviews, Roche, 1-18. 1978. 12. Kutsky, R.J., Handbook of Vitamins, Mineral and Hormones. 2th Ed.VNR. 1981, New York, 157-207. 13. Çetinkaya, N., Özcan, H., Investigation of Seasonal Variations in Cow Serum Retinol and β-Carotene by High Per- formance Liquid Chromatographic Method. Comp. Biochem. Physiol., Vol. 100 A, No: 4, 1003-1008, 1991. 14. Cerhata, D., Bauerova, A., Ginter, E., Determination of Ascorbic Acid in Blood Serum Using High-Performance Li- quid Chromatography and Its Correlation with Spectrophotometry. Caska-Slov-Farm, Vol.43(4). 166-168, 1994. 15. Catıgnanı, G.L., Simultaneous Determination of Retinol and α-Tocopherol in Serum of Plasma by Liquid Chroma- tography. Clin. Chem., 2914, 708-713, 1983. 16. Miller, K.W., Lorr, N.A., Yang, C.S., Simultaneous Determination of Plasma Retinol α Tocoferol, Iycopere, α-Caro- tene, and β-Carotene by High Performance Liquid Chromatography. Analytical Biochem., 138, 340-345, 1984. 17. Tavazzi, B., Lazzarino, G., Di-Pierro, D., Giardina, B., Malondialdehyde Production and Ascorbate Decrease are Associated to the Eperfusion of the Isolated Postischemic Rat Heart. Vol. 13(1),75-78, 1992. 18. Breyer, P.H., Gilbert, B.P., Determination of Selenium (IV) Differential Pulse Voltametry of the 3,3'–Diaminobenzi- dine piazselenol. Analytica Chimica Acta, Vol.201, 23-32. 1987. 19. Watkinson, J.H., Fluorometric Determination of Traces of Selenium. Analytical Chemistry, Vol.32, No.8, 981 - 983. 1960. 20. Whetter, P.A., Ullrey. D.E., Improved Fluorometric Method for Determining Selenium. J.Assoc. Of Anal. Chem. Vol.61, No.4. 927 - 939, 1987. 21. Işıksoluğu, M., Beslenme. İstanbul, 1989, Milli Eğitim Basımevi, 163 - 186. 22. Aras, K., Erşen, G., Karahan, S., Tıbbi Biyokimya - Vitaminler. Ankara, 1976, Ankara Üniversitesi Diş Hekimliği Fakültesi Yayınları, Sayı 4, Ankara Üniversitesi Basımevi, 190 - 211. 23. Eckler, J.A.L., Fair, J.M.S., Pharmacology Essentials, U.S.A., 1996, W.B. Saunders company, 386 - 393. 24. Jound, A.F., Data on Oxidants and Antioxidants, Bull. Eur. Pysiopathol., Respir., 22, 2535-2555, 1986. 25. Kılınç, K., Biyokimya Dergisi, 10, 60-89. 1985. 26. Underwood, E.J., Trace Elements in Human and Animal Nutrition New York, 1977, Academic Press. 302-346. 27. Quinche, J.P., Les Teneurs en Huit Elements Traces de Boletus edulis, Mycologia Helvetica, 1 (2). 89 - 94, 1983. 28. Stijve, T., Selenium Content of Mushrooms, Z. Lebensm. Unters. Forsch., 104, 201 - 203, 1977. 29. Quinche, J.P., Les Teneurs en Selenium de 95 Especes de Champignons Superieurst et de Quelgues Terres, Schweiz. Landw. Fo. Recherche agronom e Suisse, 22 (3/4), 137 - 144, 1983. 30. Oster, O., Prellwitz, W., Are Germans Selenium - Deficient? Selenium in Biology and Medicine. New York, 1989, Springer - Verlag, Berlin Heidelberg, 229 - 233. 31. Conn, E.E., Stumpf, P.K., Bruening, G., Doi, R.H., Outlines of Biochemistry 5/E, Singapore, 1987, John Wiley and Sons, 205.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK