Poliüretan köpük üzerine tutuklanmış Phanerochaete chrysosporium ME446' da gibberellik asit ve sitokinin üretimi

Phanerochaete chrysosporium ME446 gibberellik asit (GA3 eşdeğerli) ve sitokinin (zeatin eşdeğerli) üretmek üzere poliüretan köpük üzerine tutuklanmıştır. Maksimum gibberellik asit üretimi hem serbest hem de tutuklanmış hücrelerde 6. günde (sırasıyla 44.43 µg/mL ve 61.22 µg/mL) belirlenmiştir. Maksimum sitokinin üretimi ise hem serbest hem de tutuklanmış hücrelerde 12. günde (sırasıyla 9.61µg/mL and 16.33 µg/mL) saptanmıştır. Gibberellik asit ve sitokinin üretimi tutuklanmış sistemde serbest fungal miselyuma göre daha fazla verim elde edilmiştir.

Production of gibberellic acid and cytokinin by Phanerochaete chrysosporium ME446' da gibberellik asit ve sitokinin üretimi

The fungus Phanerochaete chrysosporium ME446 was entrapped on polyurethane foam to synthesize gibberellic acid (GA 3 equivalent) and Cytokinin (zeatin equivalent). Maximum gibberellic acid was defined on the 6 th day (44.43 µg/mL and 61.22 µg/mL, respectively) in both free and immobilized cells. Maximum cytokinin was defined on the 12 th day (9.61µg/mL and 16.33 µg/mL, respectively) in both free and immobilized cells. An immobilized system of gibberellic acid and cytokinin production resulted in comparatively higher yield than free fungal mycelium.

PDF

___

1. Davies, P.J., Plant Hormones, Kluwer Academic Publishers, Dordrecht/Boston/London, 1995.
2. Ünyayar, S., Topcuoğlu, Ş.F. and Ünyayar, A., A modified method for extraction and identification of indole-3-acetic acid (ıAA), gibberellic acid (GA3), abscisic acid (ABA) and zeatin produced by Phanerochaete chrysosporium ME446, Plant Physiology and Biochemistry, Special issue, 307, 1996.
3. Ergün, N., Bazı yosun ve likenlerde içsel büyüme hormonlarının (oksin, gibberellin, absisik asit ve sitokinin) üretimi, Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Antakya, 1997.
4. Gonzalez-Lopez, J., Salmeron, V., Martinez-Toledo, M.V., Ballesteros, F. and Ramos-Cormenzana, F., Production of auxins, gibberellins and cytokinins by Azotobacter vinelandii ATCC12837 in chemically-defined media and dialysed soil media, Soil Biol.Biochem., 18, 1, 119-120, 1986.
5. Jones, A. and Pharis, R.P., Production of gibberellins and bikaverin by cells of Gibberella fujikuroi immobilized in carrageenan, J.Ferment.Technol., 65,6,717-722, 1987.
6. Kumar,P.K.R. and Lonsane, B.K., Immobilized growing cells of Gibberella fujikuroi P-3 for production of gibberellic acid and pigment in batch and semi-continuous cultures, Appl.Microbiol.Biotech., 28,537-542, 1988.
7. Saucedo, J.E.N., Barbotin, J. and Thomas, D., Continuous production of gibberellic acid in a fixed-bed reactor by immobilized mycelia of Gibberella fujikuroi P-3 in calcium alginate beads, Appl.Microbiol. Biotechnol., 30,226- 233, 1989.
8. Ünyayar, A. ve Kolankaya, N., Kağıt hamuru eldesi için biyoteknolojik yaklaşım, Doğa Türk Biyoloji Dergisi, 14, 1, 51-58,1990.
9. Capdevila, C., Corrieu, G. and Asther, M.A., A feedharvest culturing method to improve lignin peroxidase production by Phanerochaete chrisosporium ıNA-12 immobilized on polyurethane foam, J. Ferment.Bioeng., 68, 1, 60-63,1989.
10. Kirkpatrick, N., Reid, I.D., Ziomek, E. and Paice, M.G., Biological bleaching of hardwood kraft pulp using Trametes (Coriolus) versicolor immobilized in polyurethane foam, Appl.Microbiol.Biotech., 33, 105-108,1990.
11. Cihangir, N. ve Aksöz, N., Tutuklanmış Aspergillus niger'den gibberellik asit eldesi, Doğa Türk Biyoloji Dergisi, 17, 175-180, 1993.
12. Kahlon, S.S. and Malhotra, S., Production of gibberellic acid by fungal mycelium immobilized in sodium alginate, Enzyme Microb.Technol., 8, 613-616, 1986.