Ratlarda desidualizasyon süresince ekstrasellüler matriksin yeniden modellenmesinde laminin ve fibronektin ile reseptör altbirimleri integrin $beta$4 ve $alpha$5' in dağılımları ve muhtemel rolleri

Gebelik sürecine hazırlanan endometriyumdaki hücreler ve bunların ürünü olan stromal matriks elemanları, endometriyal kabullenmeye bağlı olarak, yapısal farklılaşmaya giderler. Endometriyal desidualizasyonla ortaya çıkan bu yapısal farklılaşmada, ‘ektrasellüler matriks (ESM) proteinlerinin ve adhezyon moleküllerinin rol almaları kaçınılmaz bir olgudur’ hipotezine bağlı olarak, ESM molekülleri laminin ve fibronektin ile adhezyon proteinleri integrin α5 ve β4’ün ortaya çıkış ve değişen dağılımlarının incelenmesi ile desidualizayon ve implantasyondaki rollerinin belirlenmesi amaçlandı. Bunun için ergin 40 adet gebe hayvandan, gebeliğin 4-8. günlerinde disseke edilmek üzere 5 deney grubu oluşturuldu. İmplantasyon alanlarından alınan 6-8 µm kalınlığındaki parafin doku seri kesitleri immunohistokimyasal teknikleri ile boyandı. Desidual reaksiyon alanı (DRA) ilk olarak gebeliğin 4. gününde görüldü. Gebeliğin 4 ve 5. gününde fibronektinin DRA’ının merkezinde ve 6-8. günlerde ise primer desidual reaksiyon alanında (PDRA) yoğunlaştığı gözlendi. İntegrin α5, 6. güne kadar fibronektine benzer bir boyanma göstermesine rağmen, bu günden sonra PDRA’da oldukça zayıftı. Lamininin başlangıçta lümen ve bez bazal laminalarında ve DRA’nın periferinde yoğun olarak boyanırken, 6. günden sonra lamininin sekonder desidual reaksiyon alanında (SDRA) yoğunlaştığı ve tüm gebelik günlerinde integrin β4’e paralel bir immunoreaktiviteye sahip olduğu gözlendi. Sonuç olarak, sıçan uterusu endometriyumunda desidual reaksiyonun gebeliğin 4-8. günleri arasında görüldüğünü ve sadece laminin ve fibronektin değil, aynı zamanda integrin α5 ve β4’ün de desidual doku yapılanmasına ve implantasyon olayına aktif olarak katıldıklarını söyleyebiliriz.

Distibutions and possible roles of laminin, fibronectin and their receptor subunits integrin $beta$4 and $alpha$5 in remodelling of extracellular matrix during decidualization in rats

In the endometrium preparing for pregnancy, the stromal matrix components of the cells and their products begin structural differentiation due to endometrial receptivity. According to the hypothesis that 'extracellular matrix (ECM) proteins and adhesion molecules take roles in the structural differentiation as a result of endometrial decidualization', our aim was to determine the roles of ECM molecules laminin, fibronectin and adhesion proteins integrin a5 and b4 in the decidulization and implantation by examining the emergence and changing distribution of these proteins. In this study, five experimental groups, consisting of 40 pregnant animals on pregnancy days 4-8, were used. Paraffin tissue serial sections that were 6-8 µm in thickness from the implantation areas were stained by immunohistochemical techniques. Decidual reaction area (DRA) was first observed on pregnancy day 4. Fibronectin was observed densely in the center of the DRA on pregnancy days 4, 5 and in the primary decidual area (PDRA) on pregnancy days 6-8. Although integrin a5 had a similar staining to fibronectin up to pregnancy day 6, it was very weak in PDRA after that day. While laminin was dense in the basal lamina of the lumen and glandular epithelium and in the periphery of the DRA at the beginning, it was observed to be dense in the secondary decidual area (SDRA) after pregnancy day 6 and to have paralel immunoreactivity to integrin b4 on all pregnancy days. In conclusion, we can say that decidual reaction appears on pregnancy days 4-8 and not only laminin and fibronectin but also integrin a5 and b4 take part actively in decidual tissue formation and implantation events in the endometrium of rat uterus.

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