Pirinç Biti Sitophilus oryzae (L.) Popülasyonlarında Endosimbiyont Mikroorganizmaların Belirlenmesi

Ökaryot organizmalardaki simbiyotik bakteriler konukçu organizmalarda çok değişik etkiler gösterebilmektedirler. Moleküler biyoloji ve fonksiyonel genom bilim çalışmalarındaki hızlı ilerlemeler özellikle tarımsal ürünlerde zararlı olan böceklerin endosimbiyontlar ile kurdukları ilişkinin tam olarak aydınlatılmasında büyük önem arz etmektedir. Tarımsal ürünlerdeki önemli zararlılardan birisi de Pirinç biti Sitophilus oryzae (L.)’dir. Çalışma kapsamında Adana, Diyarbakır, Konya, Manisa ve İzmir illerinden S. oryzae popülasyonlarında endosimbiont mikroorganizmalardan Wolbachia, Rickettsia ve Spiroplasma varlıkları incelenmiştir. Rickettsia altı popülasyonun beşinde saptanmış ve enfeksiyon yüzdesinin %55,5 olduğu (popülasyonları temsil eden 36 bireyin 20’sinde) belirlenmiştir. Wolbachia altı popülasyonun dördünde saptanmış ve enfeksiyon yüzdesinin %22,2 olduğu (popülasyonları temsil eden 36 bireyin 8’inde) belirlenmiştir. Spiroplasma ise altı popülasyodan üçünde saptanmış olup enfeksiyon yüzdesi %8,3 olarak (popülasyonları temsil eden 36 bireyin 3’ünde) belirlenmiştir. Bu çalışmanın önemi, Spiroplazma’nın, ilk kez S. oryzae’da tespit edilmiş olmasıdır.

Endosymbiotic Microorganisms in Rice Weewil Sitophilus oryzae (L.) Populations

Symbiotic bacteria in eukaryotic organisms can show very different effects in the organism in which they are host. The rapid advances in molecular biology and functional genome science studies are of great importance especially in elucidating the relationship between insects harmful to agricultural products and endosymbionts. One of the important pests in agricultural products is Rice weewil Sitophilus oryzae (L.). Within the scope of this study, the presence of endosimbiont microorganisms such as Wolbachia, Rickettsia and Spiroplasma in S. oryzae populations in Adana, Diyarbakır, Konya, Manisa and İzmir provinces were investigated. Rickettsia was detected in five out of six populations and the percentage of contamination was found to be 55.5% (20 out of 36 individuals representing populations). Wolbachia was detected in four of the six populations and the percentage of contamination was found to be 22.2% (8 of 36 individuals representing the populations). Spiroplasma was detected in three out of six populations and the percentage of contamination was 8.3% (3 out of 36 individuals representing populations). The importance of this study is that Spiroplasma has been determined within S. oryzae for the first time.

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