Ahmet DEMİRBAŞ, Hasan DURUKAN, Sevgi DURNA DAŞTAN, Erol DÖNMEZ

Sivas İlinde Doğal Olarak Yetişen ve Yenilebilir Bir Bitki Olan Evelik (Rumex crispus) Bitkisinin Biyoaktivitesi ve Terapotik Nitelikleri

Bitkilerden elde edilen ekstrelerin birçoğunun biyolojik etkileri ve etki mekanizmaları hakkındaki bilimsel veriler hala yetersiz olmakla birlikte bu konuya olan ilgi her geçen gün artmaktadır. Bu çalışmada, Rumex crispus (Evelik) bitkisi mevsiminde Sivas yöresinden toplanarak kurutulmuş ve sonrasında yapraklı kısımlarından etanol, metanol ve su ekstraksiyonları elde edilmiştir. Çalışmanın amacı, elde edilen her bir ekstrenin bazı mikroorganizmalar üzerindeki antimikrobiyal aktivitelerinin değerlendirilmesi, toplam antioksidan seviyesi (TAS), toplam oksidan seviyesi (TOS), oksidatif stres indeksi (OSI) değerlerinin ve çeşitli hücre hatları üzerindeki anti-kanserojen niteliklerinin ortaya konulmasıdır. TAS, TOS ve OSI değerlerini tespit etmek için Rel Assay Diagnostics kitler kullanılmıştır. Bitki ekstrelerinin mikroorganizmalara karşı minimum inhibisyon konsantrasyonunu (MIC) belirlemek amacıyla mikrodilüsyon broth yöntemi kullanılmıştır. Çalışmada antimikrobiyal aktivite analizleri, Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 11778), Klebsiella pneumonia (ATCC 13883), Candida albicans (ATCC 10231) ve Candida tropicalis (DSM 11953) mikroorganizmaları kullanılarak yapılmıştır. Normal fare fibroblast hücre hattı ve 3 farklı insan kanser hücre hattında MTT yöntemi ile bitki özütlerinin sitotoksik aktivitesi belirlenmiştir. Sonuç olarak, evelik bitkisinin etanol, metanol ve su ekstraklarının denendiği mikroorganizmalar üzerinde antimikrobiyal aktivitesinin bulunmadığı, yüksek düzeyde antioksidan aktiviteye sahip olduğu ve bazı hücre kültürlerinde sitotoksik aktivitesinin olduğu tespit edilmiştir.

Bioactivity and Therapeutic Properties of Evelik (Rumex crispus), A Naturally Growing and Edible Plant in Sivas Province

Although scientific data on the biological effects and mechanisms action of many extracts from plants are still insufficient, interest in this issue is increasing day by day. In this study, Rumex crispus (Evelik) was collected from Sivas region during the season and dried, and then ethanol, methanol and water extractions were made from leafy parts. The aim of the study was to evaluate the antimicrobial activities of each extract on some microorganisms, total antioxidant level (TAS), total oxidant level (TOS), oxidative stress index (OSI) values and anticarcinogenic properties on various cell lines. Rel Assay Diagnostics kits were used to determine TAS, TOS and OSI values. Microdilution broth method was used to determine the minimum inhibition concentration (MIC) of plant extracts against microorganisms. Antimicrobial activity assays in the study were used Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 11778), Klebsiella pneumonia (ATCC 13783) Candida albicans (10231) and Candida tropicalis (DSM11953). Cytotoxic activity of plant extracts was determined by MTT method in normal Mouse fibroblast cell line and 3 different human cancer cell line. As a result, it was found that ethanol, methanol and water extracts of domestic plants do not have antimicrobial activity on microorganisms we tested, they have high antioxidant activity and have cytotoxic activity in some cell cultures.

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