Modifiye Atmosfer Paketli Sığır Kıyma ve Kuşbaşı Örneklerinde Listeria monocytogenes ve Serotiplerinin Belirlenmesi
Bu çalışma modifiye atmosfer paketli sığır kıyma ve kuşbaşı örneklerinde Listeria monocytogenes’in varlığı, serotipleri ve çeşitli antibiyotiklere karşı dirençliliğinin belirlenmesi amacıyla yapılmıştır. 50 MAP sığır kıyma örneğinin 5’inin (%10), 50 MAP sığır kuşbaşı örneğinin ise 3’ünün (%6) L. monocytogenes pozitif olduğu belirlenmiştir. PCR yöntemi ile hlyA geni varlığı araştırılan MAP sığır kıyma ve kuşbaşı örneklerinden elde edilen 11 L. monocytogenes izolatının tamamının (%100) bu gene sahip olduğu belirlenmiştir. Yapılan serotiplendirme sonucunda MAP sığır kıyma örneklerinden elde edilen 8 izolatın 3’ünün L. monocytogenes 1/2a, 3’ünün 1/2b, 2’sinin ise 4b olduğu saptanmıştır. Ayrıca MAP sığır kuşbaşı örneklerinden elde edilen 3 izolattan ise 1’inin L. monocytogenes 1/2b, 1’inin 1/2c ve 1’inin de 4b olduğu tespit edilmiştir. PCR ile doğrulanan 11 L. monocytogenes izolatının, 1’inin (%9) ampisiline, 2’sinin (%18,2) kloromfenikole, 3’ünün (%27,2) eritromisine, 4’ünün (%36,3) oksitetrasikline, 4’ünün (%36,3) penisilin G’ye, 6’sının (%54,5) tetrasikline ve 3’ünün (%27,2) de vankomisine karşı dirençli olduğu belirlenmiştir. Elde edilen L. monocytogenes izolatlarının antibiyotik dirençlilik testi sonucunda bir veya birden fazla antibiyotiklere karşı dirençli olduğu saptanmıştır. Sonuç olarak antibiyotiklere dirençli suşların gelişmesinin önlenmesi amacıyla bilinçsiz antibiyotik kullanımı önlenmesine ilişkin resmi otorite tarafından ulusal kalıntı izleme programının etkin olarak yürütülmesi büyük önem taşımaktadır.
Determination of Listeria monocytogenes and Serotypes in Modified Atmosphere Packed Ground and Cubed Beef Samples
This study was conducted to determine the Listeria monocytogenes’s presence, serotypesand resistance against various antibiotics in modified atmosphere packaged (MAP)ground and cubed beef samples. Five of ground (5/50-10%) and 3 of cubed beef samples(3/50-6%) were identified as L. monocytogenes positive in MAP samples. Eleven L.monocytogenes isolates that obtained from samples being investigated in term of hlyAgene by PCR method have verified that this gene (100%). In serotyping results, 3 of 8isolate that obtained from MAP ground beef samples were 1/2a, the other 3 isolate was1/2b and the remaining 2 isolate was 4b. Also, 1 of 3 isolate that obtained from MAPcubed beef samples were 1/2b, the other one isolate was 1/2c and the last one was 4b.One isolate against (9%) ampicillin, 2 isolate against (18.2%) chloramphenicol, 3 isolateagainst (27.2%) erythromycin, 4 isolate against (36.3%) oxytetracycline and 4 isolateagainst (36.3%) penicillin G, 6 isolate against (54.5%) tetracycline and 3 isolate against(27.2%) vancomycin was resistant in 11 L. monocytogenes isolates that confirmed byPCR. The L. monocytogenes isolates were found to be resistant to one or more antibioticsin antibiotic-resistance test results. In conclusion, applying of national residue monitoringprogram by official authority for prevention of intensive antibiotic use in order to preventthe development of resistant strains to antibiotics has great importance.
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- Akkaya L, Alisarlı M, Çetinkaya Z, Kara R, Telli R. 2008a.
Occurrence of Escherichia coli O157:H7/O157, Listeria
monocytogenes and Salmonella spp. in beef slaughterhouse
environments, equipment and workers. Journal of Muscle
Foods 19(3): 261‐274.
- Akkaya L, Alisarlı M, Cetinkaya Z, Telli R, Gök V. 2008b. The
prevalence of Escherichia coli O157:H7/O157, Listeria
monocytogenes and Salmonella spp. on bovine carcasses in
Turkey, Journal of Muscle Foods 19(4): 420‐ 429.
- Anonymous 1995. Microbiology of food and animal feeding
stuffs Horizontal method for the detection and enumeration
of L. monocytogenes Part1: Detection method. ISO11290‐1.
- Anonymous 1996. Cell separation and protein purification.
Technical handbook. 2nd Ed. Dynal A.S., Norway Printed:
02 96,1996. http://tools.invitrogen.com/content/sfs/manuals/
710%2006.Dynabeads%20anti%20 Listeria (rev004).pdf
- Ayaz ND, Erol İ. 2011. Serotype distribution of Listeria
monocytogenes isolated from turkey Meat by multiplex
PCR in Turkey. Journal of Food Safety 31: 49-153.
- Aznar R, Alarcon B. 2003. PCR detection of Listeria
monocytogenes: a study of multiple Factors affecting
sensitivity. Journal of Applied Microbiology 95: 958‐966.
- Barakat RK, Harris LJ. 1999. Growth of Listeria monocytogenes
and Yersinia enterocolitica on cooked modified-atmospherepackaged
poultry in the presence and absence of a naturally
occurring microbiota. Applied and Environmental
Microbiology 65 (1): 342-345.
- Barbuddhea SB, Malika SVS, Bhilegaonkar KN, Kumar P,
Guptab LK. 2000. Isolation of Listeria monocytogenes and
Anti-listeriolysin o detection in sheep and goats. Small
Ruminant Research 38: 151‐155.
- Bohnert M, Dilasser F, Dalet C, Mengaud J, Cossart P. 1992.
Use of specific oligonucleotides for direct enumeration of
Listeria monocytogenes in food samples by colony
hybridization and rapid detection by PCR. Research
Microbiol 143: 271‐280.
- Ciftcioglu G.1992. İstanbul piyasasındaki kıyma, sucuk ve tavuk
eti örneklerinde Listeria türlerinin mevcudiyetinin
araştırılması. Istanbul Universitesi Saglık Bilmileri
Enstitüsü. Doktora Tezi. Istanbul.
- CLSI. 2012. Clinical and Laboratory Standards Institute.
Methods for Antimicrobial Dilution and Disk Susceptibiltiy
Testing of Infrequently Isolated or Fastidious Bacteria.
Second edition. Approved Guideline M45‐A2, Wayne, PA,
USA.
- Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P. 2004.
Differentiation of the majör Listeria monocytogenes
serovars by Multiplex PCR. Journal of Clinical
Microbiology 42(8): 3819‐3822.
- Fantelli K, Stephan R. 2001. Prevalence and characteristics of
shigatoxin‐producing Escherichia coli and Listeria
monocytogenes strains isolated from minced meat in
Switzerland. International Journal of Food Microbiology 70:
63‐69.
- Farber JM, Peterkin PI. 1991. Listeria monocytogenes, a foodborne
pathogen. Microbiology Review 55: 476‐511.
- Fernandez PS, George SM, Sills CC, Peck MW. 1997.
Predictive model of the effect of CO2, pH, temperature and
NaCl on the growth of Listeria monocytogenes.
International Journal of Food Microbiology 37(1): 37‐45.
- Harakeh S, Saleh İ, Zouhairi O, Baydoun E, Barbour, Alwan N.
2009. Antimicrobia resistance of Listeria monocytogenes
isolated from dairy based products. Science of the Total
Environment 407: 4022-4027.
- Hart CD, Mead GC, Norris AP. 1991. Effects of gaseous
environment and temperature on the storage behaviour of
Listeria monocytogenes on chicken breast meat. Journal of
Applied Bacteriology 70: 40-46.
- Hof H, Rocourt J. 1992. Is any strain isolated detected in food a
health risk? International Journal of Food Microbiology 16:
683–692.
- Holzapfel WH, Becker B. 2007. Listeria monocytogenes, an
important concern for the meat industry, International
Symposium on Meat safety: From Abattoir to Consumer,
Valencia‐Spain, 14–15 February 2007, Absracts of Invited
Lectures. [http://www.meat‐ims.org/symposium_proceeding.pdf].
- Jay JM. 2000. Modern Food Microbiology. Sixth Edition,
Maryland. pp: 485‐510.
- Jemmi T, Stephan R. 2006. Listeria monocytogenes: food‐borne
pathogen and hygiene indicator. Revue scientifique et
technique‐Office International des Epizooties 25(2): 571‐580.
- Liu D, Lawrence ML, Wiedmann M, Gorski L, Mandrell RE.
2006. Listeria monocytogenes subgroups IIIA, IIIB, and IIIC
delineate genetically distinct populations with varied
virulence potential. Journal of Clinical Microbiology 4229‐
4233.
- Luppi A, Bucci G, Maini P, Rocourt J. 1998. Ecological survey
of Listeria in the Ferrara area (northern Italy). Zentralbl
Bakteriol Mikrobiol Hyg A 269: 266‐275.
- McGowan AP, Bowker K, McLauchlin J, Bennett PM, Reeves
DS. 1994. The occurrence and seasonal changes in the
isolation of Listeria spp. in shop bought food stuffs, human
feces, sewage and soil from urban sources. International
Journal of Food Microbiology 21: 325‐334.
- Niederhiauser C, Candrian U, Hofelein C, Jermini M, Buhler
HP, Luthy J. 1992. Use of polymerase chain reaction for
detection of Listeria monocytogenes in Food. Applied and
Environmental Microbiology 58(5): 1564-1568.
- Norrung B, Andersen JK, Schlundt J. 1999. Incidence and
control of Listeria monocytogenes in foods in Denmark.
International Journal of Food Microbiology 53: 195-203.
- Pothuri P, Marshall DL, McMillin KW. 1995. Combined effects
of packaging atmosphere and Lactic acid on growth and
survival of Listeria monocytogenes in crayfish tail meat at
4°C. Journal of Food Protection 59: 253-256.
- Pesavento G, Ducci B, Nieri D, Comodo N, Lo Nostro A. 2010.
Prevelance and antibiotic susceptibilty of Listeria spp.
isolated from raw meat and retaik foods. Food Control 21:
708-713.
- Pincus DH. 2010. Microbial identification using the Biomerieux
VITEK 2 system. Hazelwood, MO, USA
www.pda.org/bookstore 2010.
- Rahimi E, Ameri M, Momtaz H. 2010. Prevelance and
antimicrobial resistance of Listeria Species isolated from
milk and dairy products in Iran. Food Control 21: 1446‐1452.
- Roberts A, Nightingale K, Jeffers G, Fortes E, Kongo JM,
Wiedmann, M. 2006. Genetic and phenotypic
characterization of L. monocytogenes lineage III.
Microbiology152: 685-693.
- Samadpour M, Barbour MW, Nguyen T. 2006. Incidence of
enterohemorrhagic Escherichia coli, Escherichia coli O157,
Salmonella and Listeria monocytogenes in retail fresh
ground beef, sprouts and mushrooms. Journal of Food
Protection 69: 441‐443.
- Seeliger HPR, Höhne K. 1979. Serotyping of Listeria
monocytogenes and related species. Methot Microbiology,
13: 31‐49.
- Sireli T, Erol İ. 1999. Hazır kıymalarda Listeria türlerinin
araştırılması. Turkish Journal of Veterinary Animal Science,
23: 373‐80.
- Thevenot D, Delignette‐Muller ML, Christieans S, Vernozy‐
Rozand C. 2005. Prevalence of Listeria monocytogenes in
13 dried sausage processing plants and their products.
International Journal of Food Microbiology, 102: 85‐94.