Modifiye Atmosfer Paketli Sığır Kıyma ve Kuşbaşı Örneklerinde Listeria monocytogenes ve Serotiplerinin Belirlenmesi

Bu çalışma modifiye atmosfer paketli sığır kıyma ve kuşbaşı örneklerinde Listeria monocytogenes’in varlığı, serotipleri ve çeşitli antibiyotiklere karşı dirençliliğinin belirlenmesi amacıyla yapılmıştır. 50 MAP sığır kıyma örneğinin 5’inin (%10), 50 MAP sığır kuşbaşı örneğinin ise 3’ünün (%6) L. monocytogenes pozitif olduğu belirlenmiştir. PCR yöntemi ile hlyA geni varlığı araştırılan MAP sığır kıyma ve kuşbaşı örneklerinden elde edilen 11 L. monocytogenes izolatının tamamının (%100) bu gene sahip olduğu belirlenmiştir. Yapılan serotiplendirme sonucunda MAP sığır kıyma örneklerinden elde edilen 8 izolatın 3’ünün L. monocytogenes 1/2a, 3’ünün 1/2b, 2’sinin ise 4b olduğu saptanmıştır. Ayrıca MAP sığır kuşbaşı örneklerinden elde edilen 3 izolattan ise 1’inin L. monocytogenes 1/2b, 1’inin 1/2c ve 1’inin de 4b olduğu tespit edilmiştir. PCR ile doğrulanan 11 L. monocytogenes izolatının, 1’inin (%9) ampisiline, 2’sinin (%18,2) kloromfenikole, 3’ünün (%27,2) eritromisine, 4’ünün (%36,3) oksitetrasikline, 4’ünün (%36,3) penisilin G’ye, 6’sının (%54,5) tetrasikline ve 3’ünün (%27,2) de vankomisine karşı dirençli olduğu belirlenmiştir. Elde edilen L. monocytogenes izolatlarının antibiyotik dirençlilik testi sonucunda bir veya birden fazla antibiyotiklere karşı dirençli olduğu saptanmıştır. Sonuç olarak antibiyotiklere dirençli suşların gelişmesinin önlenmesi amacıyla bilinçsiz antibiyotik kullanımı önlenmesine ilişkin resmi otorite tarafından ulusal kalıntı izleme programının etkin olarak yürütülmesi büyük önem taşımaktadır.

Determination of Listeria monocytogenes and Serotypes in Modified Atmosphere Packed Ground and Cubed Beef Samples

This study was conducted to determine the Listeria monocytogenes’s presence, serotypesand resistance against various antibiotics in modified atmosphere packaged (MAP)ground and cubed beef samples. Five of ground (5/50-10%) and 3 of cubed beef samples(3/50-6%) were identified as L. monocytogenes positive in MAP samples. Eleven L.monocytogenes isolates that obtained from samples being investigated in term of hlyAgene by PCR method have verified that this gene (100%). In serotyping results, 3 of 8isolate that obtained from MAP ground beef samples were 1/2a, the other 3 isolate was1/2b and the remaining 2 isolate was 4b. Also, 1 of 3 isolate that obtained from MAPcubed beef samples were 1/2b, the other one isolate was 1/2c and the last one was 4b.One isolate against (9%) ampicillin, 2 isolate against (18.2%) chloramphenicol, 3 isolateagainst (27.2%) erythromycin, 4 isolate against (36.3%) oxytetracycline and 4 isolateagainst (36.3%) penicillin G, 6 isolate against (54.5%) tetracycline and 3 isolate against(27.2%) vancomycin was resistant in 11 L. monocytogenes isolates that confirmed byPCR. The L. monocytogenes isolates were found to be resistant to one or more antibioticsin antibiotic-resistance test results. In conclusion, applying of national residue monitoringprogram by official authority for prevention of intensive antibiotic use in order to preventthe development of resistant strains to antibiotics has great importance.

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Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: Aylık
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)
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