Çipura (Sparus aurata, Linneaus, 1758) Spermasının Kısa Süreli Saklanması ve Spermatolojik Özellikleri ile Ebeveyn İlişkilerinin Araştırılması
Araştırmada İzmir ili Balıklıova beldesinde bulunan bir üretim çiftliğinden temin edilen çipuralardan alınan sperm örnekleri 0°C’de buz içinde muhafaza edildikten sonra her altı saatte bir faz kontrast tip mikroskopta incelenmiştir. Çalışmada sperm muhafaza süreleri, hız, konsantrasyon, meristik karakterler ve sperm hacmi ile ilgili datalar elde edilmiş ve bu sonuçlar ebeveyn ile ilişkilendirilmiştir. Denemelerde kullanılan balıkların ağırlıkları 405-625 g, boyları ise 25-37 cm sperm hacmi 3,1-8,3 ml.kg-1 olarak tespit edilmiştir. En yoğun konsantrasyon 5,35x109 spz.ml-1 ile 2 numaralı denekte, en düşük sperm konsantrasyonu 0,16×109 spz.ml-1 ile 24 numaralı denekte saptanmıştır. Çalışma süresince tüm denek ve zamanlarda baş boyuna endeksli en yüksek hız 35.5 baş boy.sn-1 (210,16 μm.sn-1), en düşük hız 2,6 baş boy.sn-1 (15,39 μm.sn-1) olarak belirlenmiştir. Çalışma toplam 126 saat sürmüştür. En kısa muhafaza süresi 26-50 saat, en uzun muhafaza süresi 126-150 saat olarak tespit edilmiştir.
Short Term Storage of Gilthead Sea Bream (Sparus aurata, Linneaus, 1758) Spermatazoa and Investigation of Spematological Characteristic with Parental Relationships
Sperm samples taken from gilthead sea bream obtained from a production farm located in the province of Balıklıova in İzmir were examined under one phase contrast type microscope every six hours after they were kept in ice at 0°C. Data related to sperm maintenance durations, speed, concentration and sperm volume were obtained in the study and these results were related to the parent. The fish weighed 405-625 g and the sperm volume 25-37 cm was 3.1-8.3 ml.kg-1. The most intense concentration was found in the second experiment with 5.35x109 spz.ml-1 and the lowest sperm concentration in the second test with 0.16×109 spz.ml-1. During the study, the highest head speed index was determined for all subjects and time as 35.5 head height.sn-1 (210.16 μm.sn-1) and the lowest head speed was 2.6 head height.sn-1 (15.39 μm.sn-1). The study lasted a total of 126 hours. The shortest storage period is 26-50 hours and the longest storage period is 126-150 hours.
___
- Akash N. 2001. Preservation of gametes and fertilization
methodology in fringe-lipped carp, (Labeo fimbriatus B.).
MFSc thesis, University of Agricultural Sciences,
Bangalore, India, p: 54.
- Asturiano JF, Sorbera LA, Carrillo M, Zanuy S, Ramos J,
Navarro JC, Bromage N. 2001. Reproductive performance
in male European sea bass_Dicentrarchus labrax, L./fed two
PUFA-enriched experimental diets: a comparison with
males fed a wet diet. Aquaculture 194: 173–190.
- Babiak I, Glogowski J, Dobosz S, Kuzmimski H, Goryczko K.
2002. Semen from rainbow trout produced using
cryopreserved spermatozoa is more suitable for
cryopreservation. Journal of Fish Biology, 60: 561–570.
- Barbato F, Canese S, Moretti F, Misiti S, Laconi F, Rana K.
2003. Preliminary experiences for cryopreservation of
Sparus aurata and Diplodus puntazzo semen. Technology of
Aquaculture in the Mediterranean, p: 281-287.
- Basavaraja N, Hedge SN. 2005. Cryopreservation of the
endangered mahseer (Tor khudree) spermatozoa: Effect of
extender composition, cryopreservation, cryoprotectant,
dilution ratio and storage period on post thaw viability.
Büyükhatipoğlu S, Holtz W. 1978. Preservation of trout sperm
in liquid and frozen state Aquaculture, 14: 49-56.
- Cabrita E, Alvarez R, Anel L, Rana KJ, Herraez MP. 1998.
Sublethal damage during cryopreservation of rainbow trout
sperm. Cryobiology, 37: 245–253.
- Cabrita E, Robles V, Rebordinos L, Sarasquate C, Herraez MP.
2005. Evaluation of DNA damage in rainbow trout
(Oncorhynchus mykiss) and gilthead sea bream (Sparus
aurata) cryopreserved sperm. Cryobiology, 50: 144-153.
- Chen, S. Ji, X. Yu, G. Tian, Y. Sha, Z. 2004. Cryopreservation
of sperm from turbot (Scophthalmus maximus) and
application to large- scale fertilization. Aquaculture, 236,
547-556.
- Canyurt MA, Akhan S, Takma Ç. 2003. Gökkuşağı alabalığı
(Oncorhynchus mykiss W.) spermlerinin kısa süre
saklanması üzerine bir çalışma. E.U. Journal of Fisheries &
Aquatic Sciences, Cilt/Volume 20, Sayı/Issue (3-4): 537 – 542.
- Chereguini O, Garcı´a de la Banda I, Rasines I, Fernandez A.
1999. Artificial fertilization in turbot, (Scophthalmus
maximus L.): different methods and determination of the
optimal sperm-egg ratio. Aquaculture Research, 30: 319–324.
- Dreanno C, Cosson J, Suquet M, Seguin F, Dorange G, Billard
R. 1999. Nucleotide content, oxydative phosphorylation,
morphology, and fertilizing capacity of turbot (Psetta
maxima) spermatozoa during the motility period. Molecular
Reproduction And Development, 53: 230-243.
- Fabbrocini A, D’Adamo R, Pelosi S, Oliveira LFJ, Del Prete F,
Silvestri F, Vitiello V, Sansone G. 2015. Sperm motility
evaluation following long-term storage (5 years) of
cryopreservedsea bream (Sparus aurata L., 1758) semen. J.
Appl. Ichthyol, 31 (Suppl. 1) (2015), 104–107.
- Fauvel C, Omnes MH, Suquet M, Normant Y. 1992. Reliable
assessment of overripening in turbot (Scophthalmus
maximus) by a simple pH measurement. Aquaculture, 117,
107-113.
- Fauvel C, Suquet M, Dreanno C, Zonno V, Menu B. 1999.
Cryopreservation of the sea bass (Dicentrarchus labrax)
spermatozoa in experimental and production simulating
conditions. Aquat. Living Resour, 11: 387-394.
- Felip A, Zanuy S, Carrillo M. 2006. Comparative analysis of
growth performance and sperm motility between precocious
and non-precociousmales in the European sea bass.
(Dicentrarchus labrax L.) Aquaculture, Volume: 256 Issues:
1-4 Pages: 570-578.
- Geffen AJ, Evans JP. 2000. Sperm traits and fertilization
success of male and sex-reversed female rainbow trout
(Oncorhynchus mykiss). Aquaculture, 182: 61-72.
- Glogowski J, Kwasnik M, Piros B, Dabrowski K, Goryczko K,
Dobosz S, Kuzminski H, Ciereszko A. 2000.
- Characterization of rainbow trout milt collected with a
catheter: semen parameters and cryopreservation success.
Aquaculture, Research 31: 289-296.
- Harvey B, Kelley RN, Ashwood-Smith MJ. 1984.
- Cryopreservation of zebra fish spermatozoa using methanol.
Can J, 2001 1984; 60: 1867-1870.
- Hulata G, Rothbard S. 1979. Cold storage of carp semen for
short periods. Aquaculture, 16: 267-269.
- Lahnsteiner F, Patzner RA. 1998. Sperm motility of the marine
teleosts Boops boops, Diplodus sargus, Mullus barbatus and
Trachurus mediterraneus. Journal of Fish Biology, 52: 726-
742.
- Lahnsteiner F. 2000. Semen cryopreservation in the salmonidae
and in the Northern pike. Aquaculture Research, 31: 245-
258.
- Linhart O, Rodina M, Flajshans M, Gela D, Kocour M. 2005.
Cryopreservation of European catfish Silurus glanis sperm:
Sperm motility, viability, and hatching success of embryos.
Aquaculture, 235: 619–632.
- Mylonas C, Papadaki M, Divanach P. 2003. Seasonal changes in
sperm production and quality in the red porgy (Pagrus
pagrus L.). Aquaculture Research, 34: 1161-1170.
- Rolende. 2004. State of Aquaculture On The West Coast.
Annual Report.
- Sansone G, Fabbrocini A, Leropoli S, Langellotti A, Occidente
M, Matassino D. 2002. Effects of extender composition,
cooling rate, and freezing on the motility of sea bass
(Dicentrarchus labrax, L.) spermatozoa after thawing.
Cryobiology, 44: 229–239.
- Scott AP, Baynes SM. 1980. A review of the biology, handling
and the storage of salmonid spermatozoa. Journal of Fish
Biology, 17: 707-739.
- Sunitha MS, Jayaprakas V. 1997. Infuence of pH, temperature,
salinity and media on activation of motility and short-term
preservation of spermatozoa of an estuarine fish, Mystus
gulio. Indian Journal of Marine Sciences, 26: 361-365.
- Suquet M, Dreanno C, Fauvel C, Cosson J, Billard R. 2000.
Cryopreservation of sperm in marine fish. Aquaculture
Research, 31: 231-243.
- Taddei AR, Barbato F, Abelli L, Canese S, Moretti F, Rana J,
Fausto AM, Mazini M. 200. Is cryopreservation a
homogeneous process? Ultrastructure and motility of
untreated, prefreezing, and postthawed spermatozoa of
(Diplodus puntazzo C.). Cryobiology, 42: 244-255.
- TÜİK. Türkiye İstatistik Kurumu Su Ürünleri İstatistikleri.
2017. http://www.tuik.gov.tr/PreTablo.do?alt_id=1005.
- Zilli L, Schiavone R, Storelli C, Vilella S, 2008. Molecular
Mechanisms Determining Sperm Motility Initiation in Two
Sparids (Sparus aurata and Lithognathus mormyrus).
Biology of Reproduction, Volume 79, Issue 2, 1 August
2008, Pages 356–366, https://doi.org/10.1095/biolreprod.
108.068296.