The aim of this research was to examine the larvicidal activity of essential oil (EO) extracted from nutmeg (Myristica fragrans Houtt) leaves and fruits by steam distillation, and to analyze its chemical compounds. The EO yield of nutmeg leaves and fruits collected from the same tree was 0.66% and 0.30%, respectively. Larvicidal tests with the EO were carried out against Aedes aegypti (L.) (Diptera: Culicidae). The concentrations of nutmeg EO used for the larvicidal assay were 50, 100, 150, 200, and 250 ?g/mL. The results showed that fruit oil was more toxic than the leaf oil. LC50 values of leaf and fruit EOs were 133.8 and 110.1 µg/mL, respectively. The chromatogram of GC-MS showed that the chemical components in nutmeg leaf and fruit EOs were dominated by ?-pinene, sabinene, ?-pinene, delta-3-carene, limonene, ?-phellandrene, ?-terpinolene, linalool, safrole, croweacin, and myristicin.
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Abou-elnaga ZS. 2014. Insecticidal bioactivity of eco-friendly plant origin chemicals against Culex pipiens and Aedes aegypti (Diptera : Culicidae). Journal of Entomology and Zoology Studies, 2(6): 340-347.
Asgarpanah J, Kazemivash N. 2012. Phytochemistry and pharmacologic properties of Myristica fragrans Hoyutt.: A review. African Journal of Biotechnology, 11(65): 12787- 12793.
BSN. 2006. Minyak Pala (Myristica fragrans). Standar Nasional Indonesia 06-2388. Jakarta: Badan Standardisasi Nasional (In Indonesian).
Barceloux DG. 2009. Medical Toxicology of Natural Substances: Foods, Fungi, Medicinal Herbs, Toxic Plants, and Venomous Animals. Hoboken, New Jersey: John Wiley & Sons. Pp. 67-70.
Boehme AK, Noletto JA, Haber WA, Setzer WN. 2008. Bioactivity and chemical composition of the leaf essential oils of Zanthoxylum rhoifolium and Zanthoxylum setulosum from Monteverde, Costa Rica. Natural Product Research, 22(1): 31- 36.
Brophy JJ, Goldsack RJ, Punruckvong A, Forster PI, Fookes CJR. 1997. Essential oils of the genus Crowea (Rutaceae). Journal of Essential Oil Research 9(4): 401-409.
Cavalcanti ESB, de Morais SM, Lima MAA & Santana EWP. 2004. Larvicidal activity of essential oils from Brazilian plants against Aedes aegypti L. Mem Inst Oswaldo Cruz 99(5): 541-544.
Cheng SS, Huang CG, Chen YJ, YU JJ, Chen WJ, Chang ST. 2009. Chemical compositions and larvicidal activities of leaf essential oils from two eucalyptus species. Bioresource Technology 100(1): 452-456.
de Oliveira SBV, da-Cunha EVL, de Oliveira Chaves MC, Gray AI. 1997. Croweacin from Piper marginatumo. Biochemical Systematics and Ecology, 25(5), 471-472.
Jung WC, Jang YS, Hieu TT, Lee CK, Ahn YJ. 2007. Toxicity of Myristica fragrans seed compounds against Blattella germanica (Dictyoptera: Blattellidae). Journal of Medical Entomology 44(3): 524-9.
Kementerian Pertanian Republik Indonesia. 2013. Pengembangan Tanaman Pala ( New Inisiatif ) Tahun 2013. Jakarta: Dirjen Perkebunan Kementan RI (In Indonesian).
Ketaren S. 1985. Pengantar Teknologi Minyak Atsiri. Jakarta: Balai Pustaka (In Indonesian).
Khalfallah A, Labed A, Semra Z, Ai KB, Kabouche A, Touzan R, Kabouche Z. 2011. Antibacterial activity and chemical composition of the essential oil of Ammi visnaga L. (Apiaceae) from Constantine, Algeria. International Journal of Medicinal and Aromatic Plants 1(3): 302-305.
Nurdjannah N. 2007. Teknologi Pengolahan Pala. Bogor: Badan Penelitian dan Pengembangan Pertanian (In Indonesian).
Ramdani M, Bousnina Y, Djoudi A, Chalard P, Figueredo G, Lograda T. 2015. Chemical composition, antibacterial activity and chromosome number of Algerian populations of Inula crithmoides L. Journal of Science 5(11): 976-982.
Shulgin AT. 1967. Psychotropic phenylisopropylamines derived from Apiole and Dillapiole. Nature 215: 1494-95.
Srivastava S, Gupta MM, Prajapati V, Tripathi AK, Sushil Kumar. 2001. Insecticidal activity of myristicin from Piper mullesua. Pharmaceutical Biology 39(3):226-229.
Stahl E. 1985. Analisis Obat Secara Kromatografi dan Mikroskopi. Padmawinata K & Soediro I (Translators). Bandung: Institut Teknologi Bandung. Pp. 3-17 (In Indonesian).
Sukowati S. 2010. Masalah vektor demam berdarah dengue (DBD) dan pengendaliannya di Indonesia in Pangribowo S, Tryadi A (ed). Buletin Jendela Epidemiologi 2 (In Indonesian).
Tarek N, Hassan HM, Abdelghani SMM, Radwan IA, Hammouda O. 2014. Comparative chemical and antimicrobial study of nine essential oils obtained from medicinal plants growing in Egypt. Journal of Basic and Applied Sciences 53(1): 65-103.
Wahyuni S, Hadad EA, Suparman, Mardiana. 2008. Keragaman produksi plasma nutfah pala (Myristica fragrans) di KP Cicurug. Buletin Plasma Nutfah 14(2): 68-75 (In Indonesian).
WHO. 2005. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. WHO/CDS/WHOPES/GCDPP/2005.13. http://apps.who.int/iris/bitstream/10665/69101/1/WHO_CDS_ WHOPES_GCDPP_2005.13.pdf.
Zhang H, LiuQ, CaoY, FengX, ZhengY, ZouH, LiuH, YangH, Xian M. 2014. Microbial production of sabinene a new terpene- based precursor of advanced biofuel. Microbial Cell Factories 13: 20.