Desmodesmus communis (E.Hegewald) E.Hegewald Mikroalginin Kültürü ve Biyokimyasal Özellikleri

Bu çalışmada; Trakya Bölgesi iç sularından (Bahçedere Çayı, Tekirdağ, Türkiye) izole edilen, moleküler taksonomi yöntemleri ile tanımlaması yapılan KF470792 Kabul No'lu Desmodesmus communis (E. Hegewald) E. Hegewald (Sphaeropleales) mikroalg türü için kültür ortamı ve büyüme şartları belirlenmiştir. Bu mikroalg türü, belirlenen şartlar altında (besin, pH, sıcaklık, ışık yoğunluğu ve havalandırma) kültüre edilmiş ve durgunluk fazına ulaşan kültürden besinsel ve biyokimyasal analizler için yeterli miktardaki biyokütle hasat edilerek; toplam protein, toplam yağ miktarları ile yağ asitleri ve aminoasitleri, E vitaminleri çeşit ve miktarları belirlenmiştir. Türün BG11 besin ortamında (7,5 pH, 24±2ºC, 500 ml/dak. havalandırma) 9,76x105 koloni/ml hücre yoğunluğuna, 0,762 g/l kuru biyokütle ağırlığına, 13,3 mg/l toplam klorofil a miktarına ulaştığı belirlenmiştir. Biyokütle üzerine yapılan biyokimyasal analizler sonucunda; ağırlıkça %42,59 toplam protein, %5,23 toplam yağ ve 3694,24 µg/gyağ vitamin E miktarına sahip olduğu saptanmıştır. Yağ asitleri içinde en yüksek oranın %35,18 ile linolenik asit olduğu saptanmıştır. Aminoasitler içinde en yüksek miktarda bulunan glutamik asit, 46,9 mg/g olarak belirlenmiştir.

Culture of Desmodesmus communis (E.Hegewald) E.Hegewald and Its Determination of the Biochemical Properties

In this study, culture medium and growth conditions were detected for Desmodesmus communis (E. Hegewald) E. Hegewald (Sphaeropleales) with KF470792 Accession Number that isolated from Thrace inland water (Bahçedere Stream, Tekirdağ, Turkey) and determined by molecular taxonomy techniques. The microalgae was cultured under detected conditions (nutrients, pH, temperature, light density and aeration) and when the culture was reached to stationary phase microalgae biomass was harvested for biochemical analysis. Total protein, total lipid, fatty acid and amino acid compositions, vitamin E amounts and variety were detected. Cell density was 9.76x105 colony/ml; dry biomass was 0.762 g/l; chlorophyll-a was 13.3 mg/l in BG11 culture medium (7.5 pH, 24±2ºC, 500 ml/min. aeration). According to biochemical analysis; total protein amount was 42.59% (dw/w); total lipid amount was 5.23% (dw/w) and vitamin E amount was 3694.24 µg/glipid. The most abundant fatty acid was linolenic acid (35.18%); amino acid was glutamic acid (46.9 mg/g).

PDF

___

AOAC. 2000 Official Methods of Analysis. 17th Edition vol.II. Assoc. Off. Anal.Chem, Wash. D. C.,USA.
Bardakçı B, Seçilmiş H. 2006. Isparta bölgesindeki gül yağının kimyasal içeriğinin GC-MS ve FTIR spektroskopisi tekniği ile incelenmesi. SDÜ fen edebiyat fakültesi fen dergisi (EDergi), 1: 64.
Ben-Amotz A, Avron M. 1983. Accumulation of metabolites by halotolerant algae and its industrial potential. Ann. Rev. Microbiol, 37:95-119.
Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Canadian journal of biochemistry and physiology, 37(8):911-917.
El Semary NA,. 2011. The polyphasic description of a desmodesmus spp. isolate with the potential of bioactive compounds production. Biotechnol. Agron. Soc. Environ., 15(2): 231-238.
Johnston HW. 1976. The biological and economic importance of algae, part 4: The ındustrial culturing of Algae. Tuatara, 22(2):1-108.
Köse S, Kaklıkkaya N, Koral S, Tufan B, Buruk KC, Aydın F. 2011. Commercial test kits and the determination of histamine in traditional (ethnic) fish products - evaluation against an EU accepted HPLC method. Food Chemistry, 125: 1490–1497.
Lampi AM, Kataja L, Kamal-Eldin A, Piironen V. 1999. Antioxidant activities of A- and C-tocopherols in the oxidation of rapeseed oil triacylglycerols. J Am Oil Chem Soc, 76:749-755.
Massimi R, Kirkwood AE. 2016. Screening microalgae isolated from urban storm- and wastewater systems as feedstock for biofuel. PeerJ 4:e2396. Ortiz J, Romero N, Robert P, Araya J, Lopez-Hernandez J Bozz
C. 2006. Dietary fiber, amino acid, fatty acid and tocopherol contents of the edible seaweeds ulva lactuca and durvillaea antarctica. Food Chemistry, 99: 98-104.
Prescott GW. 1973. Algae of the western great lakes area. W.M.C. Brown Company Publishers, 977 pp. Pringsheim EG. 1946. Pure cultures of algae. Cambridge Press. London, 119 p.
Rios L, Klein BC, Luz J L, Wolf Maciel MR, Maciel Filho R, 2015. Influence of culture medium on desmodesmus sp. growth and lipid accumulation for biodiesel production. Chemical Engineering Transactions, 43: 601-606.
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbio1, 111: 1 -61
Safafar H, Wagenen J, Moller P, Jacobsen C. 2015. Carotenoids, phenolic compounds and tocopherols contribute to the antioxidative properties of some microalgae species grown on industrial wastewater. Mar. Drugs 13: 7339-7356.
Samek D, Misurcova L, Machu L., Bunka F, Fisera M. 2013. Influencing of amino acid composition of green freshwater algae and cyanobacterium by methods of cultivation. Turkish Journal of Biochemistry, 38 (4):360-368.
Samorì G. 2012. Algal wastewater treatment and biomass producing potential: Nutrient removal efficiency and cell physiological responses. allma matter sttudiiorum uniiversiittà dii bollogna, facoltà di scienze matematiche fisiche e naturali, dottorato di ricerca in, scienze ambientali: tutela e gestione delle risorse naturali, Ciclo XXIV, 05/A1- Botanica, BIO/01 - Botanica Generale.
Starr RC, Zeikus JA. 1993. Utex the culture collection of algae at the university of texas at austin. J. Phycol, 29: 1-106.
Stein JR. 1973. Handbook of phycological methods: culture: methods and growth measurements. Cambridge Univ. Press, London. 7-24.
Strichland JDH, Parsons T R A. 1972. Practical handbook of seawater analysis. Fisheries Research Board of Canada Bull., 167, Ottowa, 310 p,
Taipale S, Strandberg U, Peltomaa E, Galloway AWE, Ojala A, Brett MT. 2013. Fatty acid composition as biomarkers of freshwater microalgae: analysis of 37 strains of microalgae in 22 genera and in seven classes. Aquatic Microbial Ecology, 71: 165-178.
Yaich H, Garna H, Besbes S, Paquot M, Blecker C, Attia H. 2011. Chemical composition and functional properties of ulva lactuca seaweed collected in Tunisia. Food Chemistry, 128: 895-901.