Aykut ELİÇORA, Aslı Gül AKGÜL, Cüneyt ÖZER, Meltem DİLLİOĞLUGİL, Özgür Doğa ÖZSOY, Kürşat YILDIZ, Salih TOPÇU, Deniz ŞAHİN, Hale Maral KIR, Uğur TEMEL

Sıçanlarda künt akciğer travmasına bağlı akut akciğer hasarında nitrik oksit sentaz inhibitörlerinin etkisi

Amaç: Bu çalışmada deneysel sıçan modeli üzerinde uygulanan künt akciğer travmasının akciğer dokusu ve kandaki etkileri ile Ngammanitro- L-arginin metil ester ve N-iminoetil-L-ornitin uygulamasını takiben proenflamatuvar sitokinler, oksidan-antioksidan enzimler ve histopatolojik parametreler araştırıldı. Çalışma planı: Çalışmaya 50 erişkin erkek Wistar albino sıçan (ağırlık 350-400 g) dahil edildi. Sıçanlar rastgele dört gruba ayrıldı. Kontrol dışındaki diğer tüm gruplarda orta şiddette pulmoner kontüzyon oluşturuldu. Grup 1 ve 2’ye intraperitoneal salin solüsyonu, grup 3’e 25 mg.kg-1 Ngamma-nitro-L-arginin metil ester, grup 4’e 20 mg.kg-1 N-iminoetil-L-ornitin uygulandı. Kan ve akciğer dokuları biyokimyasal ve histopatolojik açıdan incelendi. Bulgular: Malondialdehit yanıtı, müköz ve histopatolojik değerler incelendiğinde, en iyi sonuçlar istatistiksel olarak anlamlı şekilde Ngamma-nitro-L-arginin metil ester ve N-iminoetil-L-ornitin uygulanan gruplarda kaydedildi. Yarışmalı nitrik oksit sentaz inhibitorü Ngamma-nitro-L-arginin metil ester uygulanan grupta süperoksit dismutaz değerlerinde anlamlı iyileşme saptandı. Nitrik oksit değerleri N-iminoetil-L-ornitin grubunda oldukça düşük idi ancak anlamlılık saptanmadı. Sonuç: Künt akciğer travması sonrası pulmoner kontüzyonda serbest oksijen radikalleri ve lipid peroksidasyon rol oynadı. Biyokimyasal ve histopatolojik sonuçlara göre, enflamasyon etkileri hem Ngamma-nitro- L-arginin metil ester hem N-iminoetil-L-ornitin uygulaması ile azaldı ve koruyucu etkiler oluştu.

Effect of nitric oxide synthase inhibitors in acute lung injury due to blunt lung trauma in rats

Background: This study aims to investigate the effects of blunt lungtrauma performed in experimental rat model on lung tissue and bloodas well as proinflammatory cytokines, oxidant-antioxidant enzymes andhistopathological parameters after Ngamma-nitro-L-arginine methylester and N-iminoethyl-L-ornithine administration.Methods: The study included 50 adult male Wistar albino rats (weighing350 to 400 g). Rats were randomly allocated into four groups. Exceptin the control, moderate-level pulmonary contusion was created in allother groups. Intraperitoneal saline solution was performed in groups 1and 2, 25 mg.kg-1 Ngamma-nitro-L-arginine methyl ester in group 3, and20 mg.kg-1 N-iminoethyl-L-ornithine in group 4. Blood and lung tissueswere studied biochemically and histopathologically.Results: Best outcomes were recorded statistically significantly in groupswith administration of Ngamma-nitro-L-arginine methyl ester andN-iminoethyl-L-ornithine when malondialdehyde response, mucous andhistopathological values were examined. Significant improvement wasdetected in superoxide dismutase values in the group with administrationof competitive nitric oxide synthase inhibitor Ngamma-nitro-L-argininemethyl ester. Nitric oxide values were substantially decreased inN-iminoethyl-L-ornithine group, while no significance was detected.Conclusion: Free oxygen radicals and lipid peroxidation played a role inpulmonary contusion after blunt lung trauma. According to biochemicaland histopathological outcomes, effects of inflammation were decreasedand protective effects were formed with administration of both Ngammanitro-L-arginine methyl ester and N-iminoethyl-L-ornithine.

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