Kıymet Z. HALK, Zeynep KAHVECİ, İlkin ÇAVUŞOĞLU, Şahin A. SIRMALI

RESVERATROL VE DİALİL DİSÜLFİTİN SIÇAN TESTİSİNDE KADMİYUM İLE OLUŞTURULMUŞ AKUT HASARA ETKİSİ

Amaç Önemli bir çevre kirletici ve dokular üzerine zararlı et kileri olan kadmiyumun erkek fertilitesi üzerine oluş turduğu hasara karşı resveratrolün ve dialil disülfitin koruyucu etkilerinin araştırılmasıdır. Gereç ve Yöntem Yapılan deneyde Wistar albino cinsi erkek sıçan kul lanıldı ve 5 grup (A: kontrol, B1: $CdCl_2$, B2: RES + CdCl2 , B3: DDS + $CdCl_2$ , B4: RES + DDS + $CdCl_2$ ) oluşturuldu. Histolojik değişiklikleri değerlendirmek için testis dokuları, Hematoksilen ve Eozin ile boyan dı ve Modifiye Johnsen skorlaması yapıldı. Gruplarda gözlenen apoptoz, TUNEL yöntemi ve aktif kaspaz-3 immünohistokimyasal boyama yöntemi ile değerlen dirildi. Bulgular B1 grubunda, kadmiyumun seminifer tübüllerde ha sara yol açtığı, spermatogenezi durdurduğu ve germ hürcelerinde apoptozu indüklediği gözlendi. Modifi ye Johnsen skorlama sonuçlarında kontrole göre B1 grubunda anlamlı derecede azalma, apoptotik indeks sonuçlarında ise anlamlı derecede artış görüldü. B1 grubuyla B2, B3 ve B4 grupları karşılaştırıldığında; Modifiye Johnsen skorlama sonuçlarında B1 ile B2 grubu arasında anlamlı bir farklılık görülmezken, B3 ve B4 gruplarında anlamlı artış görüldü. Ayrıca apop totik indeks sonuçlarında B2 grubunda anlamlı bir farklılık görülmezken, B3 ve B4 gruplarında anlamlı azalma görüldü (p< 00,1). Sonuç Sıçan testisinde kadmiyum ile oluşturulan akut ha sarın baskılanmasında resveratrol ve dialil disülfitin rolüne yönelik bilgiler ortaya konuldu. Belirlediğimiz dozda resveratrol bu akut hasarın baskılanmasında etkili olmazken dialil disülfit ve resveratrol ile birlikte kullanımı hasarı önlemede etkili bulundu. Literatür analizimize göre bu çalışma kadmiyumun indüklediği testis hasarına karşı dialil disülfitin koruyucu etkileri nin gösterildiği ilk çalışmadır.

EFFECTS OF RESVERATROL AND DIALLYL DISULFIDE ON CADMIUM INDUCED ACUTE RAT TESTIS DAMAGE

Objective In this study, the protective effects of resveratrol and diallyl disulfide against to damage of cadmium, which is a major environmental pollutant and has harmful effects on tissues, on male fertility were investigated. Materials and Methods In the experiment, Wistar albino male rats were used and 5 groups (A: control, B1: $CdCl_2$, B2: RES + $CdCl_2$ , B3: DDS + $CdCl_2$ , B4: RES + DDS + $CdCl_2$ ) were for med. Testicular tissues were stained with Hematoxy lin and Eosin and Modified Johnsen scoring were per formed to evaluate histologic changes. Apoptosis in the groups was evaluated with TUNEL method and active caspase-3 immunohistochemistry. Results According to our findings, in group B1, cadmium ca used damage in seminiferous tubules, have stopped spermatogenesis, and induced apoptosis in germ cel ls. In the modified Johnsen scoring results, there was a significant decrease in the B1 group and a signifi cant increase in the apoptotic index results. When B1 and B2, B3 and B4 groups were compared; While the re was no significant difference between B1 and B2 groups in the modified Johnsen scoring results, there was a significant increase in B3 and B4 groups. In addition, there was no significant difference in the B2 group in apoptotic index results, but a significant dec rease was observed in B3 and B4 groups (p< 00,1). Conclusion In conclusion, the role of resveratrol and diallyl disul fide in suppressing cadmium-induced acute damage in rat testis was demonstrated. While resveratrol is not effective in suppressing this acute damage, Dial lyl disulfide and diallyl disulfide with resveratrol were found to be effective in preventing of damage. Accor ding to our literature analysis, this study is the first study showing the protective effects of diallyl disulfide against cadmium-induced testicular damage.

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