MEHMET KEMAL TÜMER, Mustafa ÇİÇEK

Investigation of Immunological Differences in Mecsina Hemostopper©, Ankaferd Blood Stopper® and Tranexamic Acid Used as Haemostatic Agents with Cell Culture Study

Objective: Hemorrhagic complications may develop in many branches ofmedicine and dentistry after or during the treatment independently of the ex tent of the procedure performed. Various biomaterialshave been used in the medical and dental practiceprocedures before and after surgical procedures. Theaim of this study was to compare the immunologicalefficacies and effects of Ankaferd Blood Stopper®(ABS), Tranexamic acid (TA) used as anti-hemorrhagicagents and Mecsina Hemostopper©(MH), a newanti-hemorrhagic agent. Method: The immortalizedHuman Umbilical Vein Endothelial Cell (HUVEC) celllines, which are human umbilical cord cells and usedin many studies on coagulation and formation of fibrin,were commercially purchased for the study. The cells,5000 cells per flask for each drug, were distributedinto the 5 groups (ABS Group, TA group, MH group,distilled water-administered negative group and controlwithout any administration). Results: Accordingto the data we obtained, Tumor Necrosis Factor Alpha(TNF-α) levels were found to be lower in the cells, towhich MH was administered at concentrations of 1:2 and 1:10, than other drug groups (p

Hemostatik Ajan Olarak Kullanılan Mecsina Hemostopper©, Ankaferd Blood Stopper® ve Traneksamik Asidin İmmunolojik Farklılıklarının Hücre Kültürü Çalışması ile Araştırılması

Amaç:Tıp ve diş hekimliğinin birçok uygulamasında, tedavi sırasında veya sonrasında görülen beklenmedik kanamalar, yapılan işlemin büyüklüğünden bağımsız olarak gelişebilecek bir komplikasyon olarak değerlendirilir. Kanamanın kontrol altına alınmasını kolaylaştırmak amacıyla cerrahi işlem öncesinde ve/veya sonrasında kullanılabilecek birçok biyomalzeme üretilmiştir. Bu çalışmada kanama durdurucu ajan olarak kullanılan Ankaferd Blood Stopper® (ABS), Traneksamik Asit (TA) ve yeni bir kanama durdurucu olan Mecsina Hemostopper®’ın(MH) immünolojik etkinlikleri ve etkilerinin karşılaştırılması amaçlanmıştır. Yöntem: Çalışmada pıhtılaşma ve fibrin oluşumu üzerinde en çok çalışma yapılan ölümsüz hücre içeriğine sahip insan umblikal kord hücreleri (HUVEC, Human Umbilical Vein Endothelial Cell ) kullanılmıştır. Her flaska her bir ilaç için 5000 hücre olacak şekilde 5 gruba (ABS grubu, TA grubu, MH grubu, distile su uygulanan negatif grup ve herhangi bir uygulama yapılmayan kontrol grubu) hücreler dağıtıldı. Bulgular: Elde edilen verilere göre 1:2 ve 1:10’ luk konsantrasyonlarda; MH uygulaması yapılan hücrelerde Tümör Nekrozis Faktör Alfa (TNF-α) seviyeleri diğer ilaç gruplarına göre daha düşüktür (p

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