Fadime Mutlu İÇDUYGU, Hale ŞAMLI, Türkkan EVRENSEL, Asuman ÖZGÖZ, Kuyaş Hekimler ÖZTÜRK, Mustafa CANHOROZ, Adem DELİGÖNÜL, Necat İMİRZALIOĞLU

ASSOCIATION BETWEEN MDR 1 (ABCB1) GENE C3435T, C1236T, G2677T/A, A2956G POLYMORPHISMS AND THE RISK OF BREAST CANCER AMONG TURKISH WOMEN

Objective The most common malignancy in women is breast cancer worldwide. Previous studies performed in dif ferent populations have suggested an association between Multi-Drug Resistance (MDR1) gene pol ymorphisms and breast cancer risk in women. The purpose of the current study is to examine relationship between MDR1 polymorphisms (C3435T, G2677T/A, C1236T, A2956G) and the risk of breast cancer in Tur kish women. Material and Methods In this study 35 breast cancer cases and 20 healt hy controls were enrolled. Identification of MDR1 genotypes was performed with the polymerase cha in reaction/restriction fragment length polymorphism (PCR-RFLP) technique. Results We observed significant difference in distribution of C3435T genotypes between the cases and the cont rols (cases, CC 37.1%, CT 28.6%, and TT 34.3%; controls, CC 25%, CT 65%, and TT 10%, p: 0.023). On the other hand, no significant differences in geno type and allele frequencies of C1236T and G2677T/A polymorphisms were observed between groups. We also found that all subjects carry AA genotype for A2956G polymorphism. Conclusion Although our study group is small, the results suggest that the MDR1 C3435T polymorphism may increase the breast cancer risk in Turkish women.

TÜRK KADINLARINDA MDR1 (ABCB1) GENİ C3435T, C1236T, G2677T/A, A2956G POLİMORFİZMLERİ İLE MEME KANSERİ RİSKİ ARASINDAKİ İLIŞKİ

Amaç Meme kanseri dünya genelinde kadınlarda en yaygın rastlanan kanser türüdür. Farklı populasyonlarda ya pılan daha önceki çalışmalarda MDR1 geni polimor fizmleri ile kadınlarda meme kanseri riski arasında iliş ki olduğu öne sürülmüştür. Mevcut çalışmanın amacı Türk kadınlarında MDR1 geni C3435T, G2677A/T, C1236T, A2956G polimorfizmleri ile meme kanseri riski arasındaki ilişkiyi araştırmaktır. Gereç ve Yöntem Bu çalışmaya 35 meme kanseri hastası ve 20 sağlıklı kontrol dahil edilmiştir. MDR1 genotiplerinin belirlen mesi amacıyla polimeraz zincir reaksiyonu/restriksi yon uzunluk polimorfizmi (PCR/RFLP) yöntemi kulla nılmıştır. Bulgular Olgu ve kontrol grubu arasında C3435T polimorfizmi nin genotipleri açısından anlamlı bir fark gözlenmiştir (olgu grubu, CC %37.1, CT %28.6 ve TT %34.3; kont rol grubu CC %25, CT %65, TT %10, p: 0.023). Diğer taraftan, C1236T ve G2677A/T polimorfizmlerinin ge notip ve allel sıklıkları gruplar arasında farklı bulun mamıştır. A2956G polimorfizmi için ise tüm çalışma grubunun AA genotipini taşıdığı belirlenmiştir. Sonuç Çalışmamızda değerlendirilen hasta sayısı az olmak la birlikte, elde ettiğimiz veriler MDR1 geni C3435T polimorfizminin Türk kadınlarında meme kanseri riski ni artırabileceğini düşündürmektedir.

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