RAPD-PCR yöntemiyle Türkiye' deki Hyacinthella Schur (Liliaceae) türleri arasındaki polimorfizim ve filogenetik ilişkilerin belirlenmesi

Bu çalışmada Rastgele Çoğaltılmış Polimorfik DNA - Polimeraz Zincir Reaksiyonu (RAPD-PCR) Hyacinthella türlerini birbirinden ayırt etmek için kullanılmıştır. Kullanılan primerler, Hyacinthella türleri arasındaki genetik uzaklıkları belirlemede faydalı olduğu tespit edilmiştir. O-3 ve O-11 primerlerine göre H. heldreichii bireyleri genetik bakımdan birbirinin aynı bulunurken, O-3, O-11 ve O-4 primerlerine göre hem türler hem de bazı türün bireyleri arasında polimorfizm olduğu belirlenmiştir. H. hispida, H. lineata ve H. glabrescens bireyleri arasında oldukça yüksek polimorfizm görülmüştür.

Anahtar Kelimeler:

rastgele çoğaltılmış polimorfik DNA, filogenetik, taksonomi, Liliaceae, polimeraz zincir reaksiyonu

Polymorphism and phylogenetic relations among Turkish Species in genus Hyacinthella (Liliaceae) as determined by RAPD-PCR

In this study, Random Amplified Polymorphic DNA - Polymerase Chain Reaction (RAPD-PCR) was used to identify Hyacinthella Schur species. Primers used were found useful to determine genetical distances among Hyacinthella species. Polymorphism was seen among both species and the individuals of some species with respect to O-3, O-11 and O-4 primers, while the individuals of Hyacinthella heldreichii were determined to be genetically the same according to the O-3 and O-11 primers used. Fairly high polymorphism was observed within the individuals of H.hispida, H.lineata and H.glabrescens.

Keywords:

random amplified polymorphic DNA, phylogenetics, taxonomy, Liliaceae, polymerase chain reaction,

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