BUZAĞI KOKSİDİOZİSİNDE LİPİD PEROKSİDASYON DÜZEYİ VE ANTİOKSİDAN ENZİM AKTİVİTELERİ

Bu çalışmada, buzağı koksidiozisinde plazma malondialdehit (MDA) düzeyi ile eritrosit süperoksit dismutaz (SOD), katalaz (CAT) ve glutasyon peroksidaz (GSH-Px) enzim aktivitelerinin değerlendirilmesi amaçlandı. Çalışmada, yaşları 2 hafta ile 6 ay arasında değişen toplam 30 adet buzağı kullanıldı. Bu hayvanların 15’i sağlıklı buzağı grubunu oluştururken, 15’i koksidiozisli buzağı grubunu oluşturdu. Hayvanların klinik muayenesi yapıldı. Parazitolojik muayene için buzağılardan dışkı numuneleri toplandı. Koksidiozisli buzağıların tümünün dışkı numunelerinde Eimeria spp. oosistleri gözlemlendi. Plazma MDA düzeyi ile eritrosit GSHPx, CAT ve SOD aktivitelerinin belirlenmesi için buzağılardan kan numuneleri toplandı. Kontrolle karşılaştırıldığında koksidiozisli buzağılarda plazma MDA düzeyinin önemli derecede daha yüksek olduğu belirlendi (p<0,05). Buna karşın, koksidiozisli buzağılarda eritrosit SOD, CAT ve GSH-Px aktiviteleri kontrol grubuna göre istatistiksel açıdan önemli oranda daha düşüktü (p<0,05). Sonuç olarak, hasta buzağılarda plazma MDA düzeyinde artış, eritrosit CAT, SOD ve GSH-Px aktivitelerinde düşme koksidiozisin lipid peroksidasyonuna yol açtığını gösterebilir. Bu durum koksidiozisli buzağılarda oksidan ve antioksidan arasındaki dengenin oksidan lehine bozulduğunu göstermektedir.

Anahtar Kelimeler:

Antioksidan enzim, buzağı, Eimeria spp., koksidiozis, lipid peroksidasyon

Lipid Peroxidation Level and Antioxidant Enzyme Activities in Calves Coccidiosis

This study was performed to evaluate the plasma malondialdehyde (MDA) level, and erythrocyte glutation peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) activities in calves with coccidiosis. In this study, 30 calves, aged between 2 weeks to 6 months were used. Fifteen of these animal were included in the healthy calves group, while the other 15 consisted of calves group with coccidiosis. Clinical examinations of the animals were carried out. Faecel samples for parasitological examination were collected from calves. Eimeria spp. oocysts were seen in the faecal samples of the all calves with coccidiosis. Blood samples from the calves were collected for determination of plasma MDA level, and erythrocyte GSH-Px, CAT and SOD activities. Plasma MDA level was significantly higher in the young cattle with ringworm compared to controls (p<0.05). However, the erythrocyte SOD, CAT and GSH-Px activities in the calves with coccidiosis was significantly lower (p<0.05) than that of the controls. In conclusion, the determination of increasing in plasma MDA levels and decreasing in erythrocytes CAT, SOD and GSH-Px activities in the infected calves suggested that coccidiosis caused lipid peroxidation. This indicated that the equilibrium between oxidants and antioxidants impaired in favour of oxidants in the calves with coccidiosis.

Keywords:

Antioxidant enzyme, calves, Eimeria spp., coccidiosis, Lipid peroxidation,

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