BEYAZ KAS HASTALIKLI KUZULARDA KALP KASI HASARININ TEŞHİSİNDE KARDİYAK TROPONİN KİT ANALİZLERİ VE SERUM ENZİM AKTİVİTELERİNİN ÖNEMİ

Bu çalışmada; kuzuların beyaz kas hastalığı sonucunda oluşan myokard dejenerasyonlarının teşhisinde, beşeri kardiyak troponin I (cTn-I) ve kardiyak troponin T (cTn-T) ve serum enzim aktivitelerinin önemlerinin araştırılması amaçlandı. Bu amaçla 20 adet beyaz kas hastalıklı, 10 adet sağlıklı kuzu kullanıldı. Kardiyak Tn-I ve cTn-T testleri 5 beyaz kas hastalıklı kuzuda pozitif belirlenirken, sağlıklı kuzuların tamamında negatif sonuçlar elde edildi. Beyaz kas hastalıklı kuzularda nekropsi bulgularında kalp ve iskelet kaslarının yer yer tebeşir beyazı görünümünde olduğu belirlendi. Yine bu kuzularda ortalama kreatin kinaz MB mass (CK-MB), laktat dehidrogenaz (LDH) ve aspartat aminotransferaz (AST) enzim serum aktiviteleri sırasıyla 216,2±33,8 U/L, 717,7±657,5 U/L ve 126,4±83,3 U/L olarak bulundu. Aynı parametreler sağlıklı kuzularda sırasıyla 171,2±60,6 U/L, 513,2±210,6 U/L ve 59,2±21,1 U/L idi. Ayrıca bu parametreler cTn pozitif vakalarda (n=5) 490,5±167,7 U/L, 3804,1±2700,3 U/L ve 734.5±226.2 U/L idi. Bu grupta enzim aktiviteleri istatistiki olarak daha yüksekti. Beyaz kas hastalıklı kuzularda makroskobik bulgular, serum enzim aktiviteleri ve cTn bulguları biribiri ile uyumluydu. Sonuçta küçük ruminantların myokardiyal hasarlarının teşhisinde cTn analiz sonuçlarının duyarlı ve özgün bir gösterge olarak kullanılabileceği kanısına varıldı.

Anahtar Kelimeler:

Beyaz kas hastalığı, kardiyak troponin-I, kardiyak troponin-T, kuzu

The Importance of Cardiac Troponin Kit Analyses and Serum Enzyme Activities in the Diagnosis of Heart Muscle Degeneration in Lambs with White Muscle Disease

The aim of this study was to investigate the value of human cardiac troponin-I (cTn-I), cardiac troponin-T (cTn-T) kits and serum enzymes activities in the diagnosis of myocardial degeneration in lambs with White Muscle Disease (WMD). For this purpose, 20 lambs with acute WMD and 10 clinically healthy lambs were used. Cardiac Tn-I and cTn-T tests were determined as positive in the only 5 lambs with WMD, but these tests were negative in blood samples collected from healthy lambs. In necropsy findings, the cardiac and skeletal muscles were observed to be affected by chalky white lesions. Mean Creatine Kinase-MB mass (CK-MB), Lactate Dehydrogenase (LDH) and Aspartate Aminotransferase (AST) enzyme activities were found as 216,2±33,8 U/L, 717,7±657,5 U/L and 126,4±83,3 U/L respectively in lambs with WMD. Same parameters were mean 171,2±60,6 U/L, 513,2±210,6 U/L and 59,2±21,1 U/L in healthy lambs, respectively. Additionally these parameters were determined as 490,5±167,7 U/L, 3804,1±2700,3 U/L and 734,5±226,2 U/L in cTn positive cases (n=5) respectively. In this group mean enzyme activities were statistically higher than in the other groups. Macroscopic findings of heart muscle, serum enzymes activities and positive cTn results were correlated in cases with WMD. In conclusion, cTn assays may be a sensitive and specific marker for small ruminants with myocardial injury.

Keywords:

White muscle disease, cardiac troponinI, cardiac troponin-T, lamb,

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