Mert PEKCAN, Ahu DEMİRTAŞ, İlksin PİŞKİN, Saad Ahmed Adam MUSA, Yasemin SALGIRLI DEMİRBAŞ, Bahri EMRE, Hakan ÖZTÜRK, Neşe TOPRAK

Yoğurt Otu (Galium aparine) ve Civan Perçemi (Achillea millefolium) Ekstraktlarının In-vitro Yarı-Sürekli Kültür Sisteminde (RUSITEC) Rumen Mikrobiyal Fermentasyonu Üzerine Etkileri

Yoğurt otu (Galium aparine) ve civan perçemi (Achillea millefolium) ekstraktlarının rumen mikrobiyal fermentasyonu üzerine etkileri ile ilgili sınırlı düzeyde deneysel veri bulunmaktadır. Bu çalışmanın amacı, yoğurt otu ve civan perçemi ekstraktlarının in vitro ruminal fermentasyon üzerine etkilerinin araştırılmasıdır. Inkübasyon denemesi uzun-süreli rumen simülasyon tekniği (RUSITEC) kullanılarak gerçekleştirilmiştir. Deneme 10 gün sürmüştür. Beş günlük bir adaptasyon periyodunu takiben fermenterler 3 gruba bölünmüştür; ilk üç fermentere ilave yapılmamış (kontrol), ikinci üç fermenterlik gruba günlük 500 mg/L yoğurt otu ekstraktı, üçüncü üç fermenterlik gruba ise günlük 500 mg/L civan perçemi ekstraktı eklenmiştir. Yoğurt otu ve civan perçemi ilaveleri, ruminal pH, toplam uçucu yağ asitleri (UYA), asetat, propiyonat ve metan üretimi, NH3-N konsantrasyonu ve toplam protozoa üzerine önemli bir etki oluşturmamıştır. Ancak her iki ekstrakt da kuru madde sindirilebilirliğini (KMS) azaltmıştır (P

Effects of Cleavers (Galium aparine) and Yarrow (Achillea millefolium) Extracts on Rumen Microbial Fermentation in In-vitro SemiContinuous Culture System (RUSITEC)

Experimental data on the effects of cleavers (Galium aparine) and yarrow (Achillea millefolium) extracts on rumen microbial fermentation arescarce. The objective of this study was to determine the effects of cleavers and yarrow extracts on in vitro ruminal fermentation. Incubationtrial was carried out using the long-term rumen simulation technique (RUSITEC). The experiment lasted 10 days. After an adaptation periodof 5 days, the fermentation vessels divided into 3 groups; first three vessels received no additives (control), second three vessels received 500mg/L cleavers extract daily, and third three vessels received 500 mg/L yarrow extract daily. Supplementations of cleavers and yarrow extractshad no significant effect on ruminal pH, total volatile fatty acids (VFA), acetate, propionate and methane production, NH3-N concentrationand, total protozoa. However, both extracts decreased dry matter digestibility (DMD) (P

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