Muhamed KATICA, Ognjenka JANKOVI, Faruk TANDIR, Ned ad GRADA EVI, Radoslav DEKI, Maja MANOJLOVI, Smiljana PARA, Ljiljana TADI - LATINOVI

The Effects of Calcium Aluminate and Calcium Silicate Cements Implantation on Haematological Profile in Rats

The objective of this study was to evaluate potential unfavourable side effects, especially on the blood corpuscles, of experimental calcium aluminate and calcium silicate cements that are applied subdermally or directly to tooth pulp. In the study, fifty-four Wistar rats were separated into two study groups (n=27 in each group). The effects of the dental cements on haematological parameters were observed three times (after 7, 15 and 30 days): erythrocytes, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, leukocytes, leucogram and poikilocytic erythrocyte forms. There were no statistically significant differences in the total number of leukocytes and percentage results of lymphocytes, and neutrophils. The study recorded lower numbers of erythrocytes, with no connection with the type of material applied to the dental pulp (First study group). Significant differences in results for the second study group were recorded on days 7-15 and 15-30 of the experiment in relation to both types of implanted cements. In the second study group there were significant differences related to both types of implanted cements, in the same periods. Lower values of erythrocytes, haemoglobin and haematocrit indicated the existence of hypochromic anaemia caused by the damaging influence of calcium aluminate and calcium silicate. Research showed that in both study groups, normocytic hypochromic anaemia existed, along with modest to expressed distribution of anulocytes and stomatocytes. According to the results of the research, the negative impact of application of Ca-silicate was recorded and was related to the appearance of hypochromic anaemia with poikilocytic forms of erythrocytes.

Kalsiyum Alüminat ve Kalsiyum Silikat Sement İmplantasyonunun Ratlarda Hematolojik Profil Üzerine Etkileri

Bu çalışmanın amacı, deneysel olarak subdermal veya direkt olarak diş pulpasına uygulanan kalsiyum alüminat ve kalsiyum silikat sementlerin, özellikle kan kürecikleri üzerindeki potansiyel olumsuz yan etkilerini değerlendirmektir. Çalışmada, elli dört Wistar rat iki çalışma grubuna ayrıldı (her grupta n = 27). Dental sementlerin hematolojik parametreler üzerindeki etkileri eritrositler, hemoglobin, hematokrit, ortalama korpüsküler volüm, ortalama korpüsküler haemoglobin, ortalama korpüsküler hemoglobin konsantrasyonu, lökositler, lökogram ve poikilositik eritrosit formlarını içerecek şekilde üç kez (7, 15 ve 30 gün sonra) gözlemlendi. Total lökosit sayısı ile lenfosit ve nötrofillerin yüzde değerlerinde istatistiksel olarak anlamlı bir fark yoktu. Çalışma, eritrosit sayısında diş pulpalarına uygulanan materyal tipiyle hiçbir bağlantısı olmayan bir azalmayı ortaya koydu (Birinci çalışma grubu). İkinci çalışma grubu için elde edilen sonuçlarda, 7-15 ve 15-30. günlerde her iki sement implant tipiyle ilişkili olan anlamlı farklılıklar saptandı. İkinci çalışma grubunun aynı periyotlatında her iki sement implant türü ile ilgili önemli farklılıklar vardı. Düşük eritrosit, hemoglobin ve hematokrit değerleri, kalsiyum alüminat ve kalsiyum silikatın zararlı etkisinden kaynaklanan hipokromik aneminin varlığını gösterdi. Bu araştırma ile her iki çalışma grubunda da anülositlerin ve stomatoitlerin orta dereceli ekspresyonunu gösteren normositik hipokromik anemi varlığı belirlendi. Araştırma sonuçlarına göre, Ca-silikat uygulamasının olumsuz etkisi saptandı ve bu durum eritrositlerin poikilositik formları ile birlikte görülen hipokromik anemi ile ilişkiliydi.

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