Haobo LI, Jiaqi XUE, Baoxiang HAN, Demtu ER

Wohlfahrtia magnifica’nın Kışlama Davranışı ve Soğuk Stresiyle İlgili Unigenleri Üzerine Çalışmalar

Bu çalışmada, Wohlfahrtia magnifica’nın kışlama davranışı ve soğuk stresi ile ilgili unigenleri incelenmiştir. Wohlfahrtia magnifica’nın pupaları sırasıyla 4°C, -5°C, -10°C, -15°C ve -24°C’de oda sıcaklığına yerleştirildi ve düşük sıcaklık indüksiyonundan sonra geri kazanım deneyi yapıldı. Pupalardan çıkan erişkin sinekler sayıldı, -5°C, -10°C, -15°C ve -24°C’deki kabuk iç pupalar fotoğraflandı ve kaydedildi. Pupalarda, oda sıcaklığında (PA), -5°C (PA1)’de ve -10°C (PA2)’de transkriptom sekanslama yapıldı. Sonuçlar, Gen ontolojisi (GO) ve Kyoto Gen ve Genom Ansiklopedisi (KEGG) ile analiz edildi ve HSP67Bc, HSP23, HSP27, HSC70-4 ve HSP70Ba, Q-PCR ile doğrulandı. Sonuçlar, PA1 ve PA2’deki ısı şoku proteinlerinin (HSP’ler) ekspresyon seviyelerinin PA’ya göre önemli ölçüde yüksek olduğunu ve HSP23, HSP27, HSP67Bc, HSP70Ba, HSP60, HSP83 ve HSC70-4 ve HSC7-5 gibi ısı şok protein homologlarının (HSC’ler) düşük sıcaklık stresi altındaki pupalarda yüksek oranda eksprese edildiğini gösterdi. GO ve KEGG’de sırasıyla 13168 ve 11161 kayıtlarına açıklama yapıldı. Q-PCR sonucu, HSP67Bc dışındaki diğer dört unigenin analiz sonuçlarının, transkriptom analiz sonuçları ile tutarlı olduğu saptandı. Bu nedenle, Wohlfahrtia magnifica’nın kışlama davranışı pupa formunda olmuştur. HSP’ler, Wohlfahrtia magnifica pupalarının kışlama sürecinde önemli rol oynamıştır.

Studies on Overwintering Behavior and Cold Stress Related Unigenes of Wohlfahrtia magnifica

The overwintering behavior and unigenes related to cold stress were studied in this paper. The pupae of Wohlfahrtia magnifica were placed at room temperature, 4°C, -5°C, -10°C, -15°C and -24°C respectively, and the recovery experiment after low temperature induction was carried out. The hatching of the pupae was counted, the shell interior pupae at -5°C, -10°C, -15°C and -24°C were photographed and recorded. Transcriptome sequencing was performed on the pupae at room temperature (PA), -5°C (PA1) and -10°C (PA2). The results were analyzed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and the HSP67Bc, HSP23, HSP27, HSC70-4 and HSP70Ba were verified by Q-PCR. The results showed that the expression level of heat shock proteins (HSPs) in PA1 and PA2 were significantly higher than in PA, and HSP23, HSP27, HSP67Bc, HSP70Ba, HSP60, HSP83 and heat shock protein homologous (HSCs) such as HSC70-4, HSC7-5 were highly expressed in the pupae under low temperature stress. 13168 and 11161 entries were annotated in GO and KEGG, respectively. Q-PCR result showed that except HSP67Bc, the analysis results of the other four unigenes were consistent with the data of transcriptome analysis. Therefore, the overwintering behavior of Wohlfahrtia magnifica was in the form of pupa. HSPs played an important role in the overwintering process of the Wohlfahrtia magnifica pupa

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