Songul DOGANAY, Nurten BAHTIYAR, Arzu SAHIN, Ozcan BUDAK

Deneysel Sepsis Modelinde Nikotinamid Adenin Dinükleotidin (NAD + ) Akut Hepatorenal Oksidatif Hasara Karşı Antioksidan ve Anti-inflamatuar Etkileri

enfl amatuvar etkilerini belirlemektir. 28 erkek Wistar-albino sıçan rast gele yedişerli dört gruba ayrıldı. Çalışma grupları; Sham grubu, sepsis grubu (CLP), sepsis + 100 mg nikotinamid uygulama grubu (CLP+N100) ve sepsis + 300 mg nikotinamid uygulama grubu (CLP+N300) şeklinde oluşturuldu. NAD + çekum perforasyonundan önce beş gün boyunca ve operasyondan 6 saat sonra intraperitoneal yoldan verildi. Operasyondan 24 saat sonra ratlardan serum, karaciğer ve böbrek dokuları alındı. Alınan doku örneklerinde MDA, GSH, CAT, TNF-α, IL-6, IL-1β, kaspaz-3 parametreleri biyokimyasal ve immünohistokimyasal metod kullanılarak ölçüldü. Histopatolojik ve immünhistokimyasal incelemede, CLP grubunun karaciğer ve böbrek dokularında TNF-α, IL-6, IL-1β ve kaspaz-3 ekspresyonlarında artışlar gözlendi ve doku morfolojisinde ciddi hasar görüldü (P

Antioxidant and Anti-Infl ammatory Effects of Nicotinamide Adenine Dinucleotide (NAD+ ) Against Acute Hepatorenal Oxidative Injury in An Experimental Sepsis Mod

The aim of this study is to determine the antioxidant and anti-infl ammatory effects of Nicotinamide Adenine Dinucleotide (NAD + ) in preventing multi-organ damage caused by sepsis. Twenty-eight male Wistar-albino rats were randomly divided into four groups. The study groups comprised Sham group, sepsis group (CLP), sepsis + 100 mg/kg NAD + (CLP+N100) and sepsis + 300 mg/kg NAD + group (CLP+N300). Sepsis was induced by the cecum ligation perforation (CLP) method. NAD + was administered intraperitoneally for five days before cecum perforation and 6 h after operation. Serum, liver and kidney tissues were taken from the rats 24 h after the operation. MDA, GSH, CAT, TNF-α, IL-6, IL-1β, and caspase-3 parameters were measured in tissue samples with biochemical and immunohistochemical methods. In the histopathological and immunohistochemical examination, increases in TNF-α, IL-6, IL-1β, and caspase-3 expressions were observed in the liver and kidney tissues of the CLP group and severe damage was seen in tissue morphology (P

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