Oğuz MERHAN, Kadir BOZUKLUHAN, Kemal VAROL, Hale ERGİN EĞRİTAĞ

Evaluation of Acute Phase Response in Blood and Milk Samples of Healthy Holstein Cattle in the Postpartum Period

Normally, birth triggers an acute phase response (APR). In particular, interleukins and proinfl ammatory cytokines released from activated leukocytes at the site of tissue damage stimulate APR. In the liver, these cytokines also stimulate acute phase proteins (APPs). APPs are one of the options used in monitoring the health status of animals. This study was evaluated in 12 healthy Holstein cattle, 3-6 years old, who calved at least once. Blood and milk samples were collected from the animals’ immediately after calving (0 th h) and on the 7th , 14th , and 21st days postpartum. Haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin (Cp), milk amyloid A (MAA), albumin, total protein (TP) and globulin levels were determined in blood serum and milk serum samples. In the findings; when the measurements of blood and milk Hp, Cp and SAA and TP values were compared; there was a statistically significant difference (P

PDF

___

1. Havekes CD, Duffield TF, Carpenter AJ, DeVries TJ: Effects of wheat straw chop length in high-straw dry cow diets on intake, health, and performance of dairy cows across the transition period. J Dairy Sci, 103 (1): 254-271, 2020. DOI: 10.3168/jds.2019-17033
2. Arslan C, Tufan T: Geçiş dönemindeki süt ineklerinin beslenmesi II. Bu dönemde görülen metabolik hastalıklar ve besleme ile önlenmesi. Kafkas Univ Vet Fak Derg, 16 (1): 159-166, 2010. DOI: 10.9775/kvfd.2009.443
3. Pascottini OB, Leroy JLMR, Opsomer G: Metabolic stress in the transition period of dairy cows: Focusing on the prepartum period. Animals, 10 (8): 1419, 2020. DOI: 10.3390/ani10081419
4. Leblanc S: Monitoring metabolic health of dairy cattle in the transition period. J Reprod Dev, 56, 29-35, 2010. DOI: 10.1262/jrd.1056s29
5. Çenesiz M, Sağkan Öztürk A, Dalğın D, Yarım GF, Çiftçi G, Özdemir R, Güzel M, Kazak F, Çenesiz S: Investigation of acute phase reactants and antioxidant capacity in calves infected with Cryptosporidium parvum. Kafkas Univ Vet Fak Derg, 23 (3): 481-485, 2017. DOI: 10.9775/ kvfd.2016.17183
6. Bozukluhan K, Merhan O, Gökçe Hİ, Öğün M, Atakişi E, Kızıltepe Ş, Gökçe G: Determination of some acute phase proteins, biochemical parameters and oxidative stress in sheep with naturally infected sheeppox virus. Kafkas Univ Vet Fak Derg, 24 (3): 437-441, 2018. DOI: 10.9775/kvfd.2017.19167
7. Erkılıç EE, Merhan O, Kırmızıgül AH, Öğün M, Akyüz E, Çitil M: Salivary and serum levels of serum amyloid a, haptoglobin, ceruloplasmin and albumin in neonatal calves with diarrhoea. Kafkas Univ Vet Fak Derg, 25 (4): 583-586, 2019. DOI: 10.9775/kvfd.2018.21424
8. Merhan O, Taşçı GT, Bozukluhan K, Aydın N: Determination of oxidative stress index and total sialic acid in cattle infested with Hypoderma spp. Kafkas Univ Vet Fak Derg, 26 (5): 633-636, 2020. DOI: 10.9775/kvfd.2020.24071
9. Basbug O, Yurdakul I, Yuksel M: Evaluation of serum amyloid A and procalcitonin in some inflammatory diseases of cattle. Kafkas Univ Vet Fak Derg, 26 (3): 397-402, 2020. DOI: 10.9775/kvfd.2019.23412
10. Humblet MF, Guyot H, Boudry B, Mbayahi F, Hanzen C, Rollin F, Godeau JM: Relationship between haptoglobin, serum amyloid A, and clinical status in a survey of dairy herds during a 6-month period. Vet Clin Pathol, 35, 188-193, 2006. DOI: 10.1111/j.1939-165x.2006.tb00112.x
11. Bertoni G, Trevisi E, Han X, Bionaz M: Effects of inflammatory conditions on liver activity in puerperium period and consequences for performance in dairy cows. J Dairy Sci, 91, 3300-3310, 2008. DOI: 10.3168/ jds.2008-0995.
12. Colombo JP, Richterich R: Zur bestimmung des caeruloplasmin im plasma [on the determination of ceruloplasmin in plasma]. Schweiz Med Wochenschr, 23, 715-720, 1964.
13. Doumas BT, Watson WA, Biggs HG: Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acta, 31, 87-96, 1971. DOI: 10.1016/0009-8981(71)90365-2
14. Jawor P, Brzozowska B, Sloniewski K, Kowalski ZM, Stefaniak T: Acute phase response in the primiparous dairy cows after repeated percutaneous liver biopsy during the transition period. Pol J Vet Sci, 19 (2): 393-399, 2016. DOI: 10.1515/pjvs-2016-0049
15. Trevisi E, Amadori M, Cogrossi S, Razzuoli E, Bertoni G: Metabolic stress and inflammatory response in high-yielding, periparturient dairy cows. Res Vet Sci, 93, 695-704, 2012. DOI: 10.1016/j.rvsc.2011.11.008
16. Nightingale CR, Sellers MD, Ballou MA: Elevated plasma haptoglobin concentrations following parturition are associated with elevated leukocyte responses and decreased subsequent reproductive efficiency in multi- parous Holstein dairy cows. Vet Immunol Immunopathol, 164, 16-23, 2015. DOI: 10.1016/j.vetimm.2014.12.016
17. Uchida E, Katoh N, Takahashi K: Appearance of haptoglobin in serum from cows at parturition. J Vet Med Sci, 55, 893-894, 1993. DOI: 10.1292/jvms.55.893
18. Chan JPW, Chang CC, Hsu WL, Liu WB, Chen TH: Association of increased serum acute-phase protein concentrations with reproductive performance in dairy cows with postpartum metritis. Vet Clin Pathol, 39, 72-78, 2010. DOI: 10.1111/j.1939-165X.2009.00182.x
19. Bossaert P, Trevisi E, Opsomer G, Bertoni G, De Vliegher S, Leroya, JLMR: The association between indicators of inflammation and liver variables during the transition period in high-yielding dairy cows: An observational study. Vet J, 192, 222-225, 2012. DOI: 10.1016/j.tvjl. 2011.06.004
20. Ceciliani F, Ceron JJ, Eckersall PD, Sauerwein H: Acute phase proteins in ruminants. J Proteomics, 75, 4207-4231, 2012. DOI: 10.1016/j. jprot.2012.04.004
21. Skinner JG, Brown RA, Roberts L: Bovine haptoglobin response in clinically defined field conditions. Vet Rec, 128, 147-149, 1991. DOI: 10.1136/vr.128.7.147
22. Szczubiał M, Dąbrowski R, Kankofer M, Bochniarz M, Albera E: Concentration of serum amyloid A and activity of ceruloplasmin in milk from cows with clinical and subclinical mastitis. Bull Vet Inst Pulawy, 52, 391-395, 2008.
23. Hussein HA, Staufenbiel R: Variations in copper concentration and ceruloplasmin activity of dairy cows in relation to lactation stages with regard to ceruloplasmin to copper ratios. Biol Trace Elem Res, 146 (1): 47-52, 2012. DOI: 10.1007/s12011-011-9226-3
24. Bayyit E, Merhan O: Normal ve güç doğum yapan ineklerde bazı akut faz proteinlerinin ve oksidatif stres düzeyinin belirlenmesi. Atatürk Universitesi Vet Bil Derg, 15 (2): 145-150, 2020. DOI: 10.17094/ ataunivbd.688400
25. Piccione G, Messina V, Schembari A, Casella S, Giannetto C, Alberghina D: Pattern of serum protein fractions in dairy cows during different stages of gestation and lactation. J Dairy Res, 78 (4): 421-425, 2011. DOI: 10.1017/S0022029911000562
26. Chorfi Y, Lanevschi‐Pietersma A, Girard V, Tremblay A: Evaluation of variation in serum globulin concentrations in dairy cattle. Vet Clin Pathol, 33 (3): 122-127, 2004. DOI: 10.1111/j.1939-165x.2004.tb00360.x
27. Grünberg W, Staufenbiel R, Constable PD, Dann HM, Morin DE, Drackley JK: Liver phosphorus content in Holstein-Friesian cows during the transition period. J Dairy Sci, 92, 2106-2117, 2009. DOI: 10.3168/ jds.2008-1897
28. Mackiewicz A: Acute phase proteins and transformed cells. Int Rev Cytol, 170, 225-300, 1997. DOI: 10.1016/s0074-7696(08)61623-x
29. Çenesiz S: The role of oxidant and antioxidant parameters in the infectious diseases: A systematic literature review. Kafkas Univ Vet Fak Derg, 26 (6): 849-858, 2020. DOI: 10.9775/kvfd.2020.24618
30. Milica KF, Jelka S, Marija SP, Jasmina DM, Milijana K, Zora M, Tatjana B: Acute phase protein response in cows with Staphylococcus aureus subclinical mastitis. Acta Vet-Beograd, 60 (2-3): 205-216, 2010. DOI: 10.2298/AVB1003205K
31. Thomas FC, Waterston M, Hastie P, Parkin T, Haining H, Eckersall PD: The major acute phase proteins of bovine milk in a commercial dairy herd. BMC Vet Res, 11:207, 2015. DOI: 10.1186/s12917-015-0533-3
32. McDonald TL, Larson MA, Mack DR, Weber A: Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum. Vet Immunol Immunopathol, 83, 203-211, 2001. DOI: 10.1016/s0165-2427(01)00380-4
33. Evkuran Dal G, Sabuncu A, Aktaran Bala D, Enginler SÖ, Çetin AC, Çelik B, Koçak Ö: Evaluation of intramammary platelet concentrate efficacy as a subclinical mastitis treatment in dairy cows based on somatic cell count and milk amyloid A levels. Kafkas Univ Vet Fak Derg, 25 (3): 357- 363, 2019. DOI: 10.9775/kvfd.2018.20982
34. Conner JG, Eckersall PD, Doherty M, Douglas TA: Acute phase response and mastitis in the cow. Res Vet Sci, 41, 126-128, 1986. DOI: 10.1016/S0034-5288(18)30585-X
35. Çenesiz S, Gürler H, Fındık A, Çiftci G, Ertekin A, Çenesiz M: Acute phase proteins in Staphylococcus aureus positive milks. Etlik Vet Mikrobiyol Derg, 29 (2): 111-115, 2018. DOI: 10.35864/evmd.513501
36. Hussein HA, Abd El-Razik KAEH, Gomaa AM, Elbayoumy MK, Abdelrahman KA, Hosein HI: Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle. Vet World, 11 (1): 34-41, 2018. DOI: 10.14202/vetworld.2018.34-41
37. Eckersall PD, Young FJ, McComb C, Hogarth CJ, Safi S, Weber A, McDonald T, Nolan AM, Fitzpatrick JL: Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Vet Rec, 148 (2): 35-41, 2001. DOI: 10.1136/vr.148.2.35
38. Eckersall PD, Young FJ, Nolan AM, Knight CH, McComb C, Waterston M,M Hogarth CJ, Scott EM, Fitzpatrick JL: Acute phase proteins in bovine milk in an experimental model of Staphylococcus aureus subclinical mastitis. J Dairy Sci, 89 (5): 1488-1501, 2006. DOI: 10.3168/jds.S0022-0302(06)72216-0
39. Dalanezi FM, Schmidt EMS, Joaquim SF, Guimarães FF, Guerra ST, Lopes BC, Cerri RLA, Chadwick C, Langoni H: Concentrations of acute-phase proteins in milk from cows with clinical mastitis caused by different pathogens. Pathogens, 9 (9): 706, 2020. DOI: 10.3390/ pathogens9090706
40. Chacornac JP, Barnouin J, Raboisson T: Automated microanalysis of plasma ceruloplasmin for the measurement of oxidase activity in cattle and sheep. Reprod Nutr Dev, 26: 417-427, 1986.
41. Gürler H, Çiftci G, Baştan A: Subklinik mastitis’in anadolu mandalarının süt kompozisyonundaki bazı biyokimyasal parametrelere etkisi. Etlik Vet Mikrobiyol Derg, 29 (2): 151-156, 2018. DOI: 10.35864/evmd.513542
42. Donley SA, Ilagan BJ, Rim H, Linder MC: Copper transport to mammary gland and milk during lactation in rats. Am J Physiol Endocrinol Metab, 283, E667-E675, 2002. DOI: 10.1152/ajpendo.00115.2002
43. Moretti DB, Santos CB, Alencar SM, Machado-Neto R: Colostrum from primiparous Holstein cows shows higher antioxidant activity than colostrum of multiparous ones. J Dairy Res, 87 (3): 356-359, 2020. DOI: 10.1017/S0022029920000813