Evren KOBAN BAŞTANLAR, Arif PARMAKSIZ, Emel ÖZKAN ÜNAL, Ahmet OYMAK, Füsun ÖZER, İnci TOGAN, Sevgin DEMİRCİ, Eren YÜNCÜ

Türkiye’den 12 Yerli, Karagül, Karacabey Merinosu ve Anadolu Yaban Koyununda (Ovis gmelinii anatolica) Y-Kromozom Polimorfizmleri

Bu çalışmada, Anadolu’dan yerli evcil koyun (Ovis aries) ırklarından 13 ırk ile kökeni Avrupa’dan olan Bandırma Merinosu koyun ırkı ve Anadolu Yaban Koyunu (Ovis gmelinii anatolica)’na ait olmak üzere toplam 182 erkek birey materyal olarak kullanılmıştır. Y kromozomunda bulunan SRY 5’promotor bölgesi ve Y kromozomuna özel bir mikrosatellit olan SRYM18 bölgeleri DNA dizi analizi yöntemleriyle incelenmiştir. SRY bölgesine ait analizlerde tüm bireylerde SNP A-oY1 alleline rastlanmıştır. SRYM18 mikrosatellit bölgesinde ise 4 farklı allel görülmüş ve toplamda 4 farklı babasal soy, haplotip, tespit edilmiştir. Yerli Anadolu ırklarında (n=143), H6 haplotipi (%80.41), H4 haplotipi (%9.09), H8 haplotipi (%8.40) ve H12 haplotipi (%2.1) belirlenmiştir. Ovis gmelinii anatolica’nın 16 bireyinde de sadece H6 haplotipi gözlenmiştir. Evcil koyunlar için haplotip frekanslarına dayalı FST değerleri hesaplanmış ve en yüksek FST değeri Karagül-Kıvırcık ve Karagül-Ovis gmelinii anatolica arasında FST değeri 0.43202 (P

Y-Chromosome Polymorphisms in 12 Native, Karagül, Karacabey Merino Breeds from Turkey and Anatolian Mouflon (Ovis gmelinii anatolica)

In this study, 182 male animals from 12 native sheep breeds, as well as Karacabey Merino and Karagül breeds of Anatolia, wild sheep AnatolianMouflon (Ovis gmelinii anatolica) were used as the study material. Based on SRY and SRYM18 regions on the Y-chromosome, haplotypes of thepopulations were analyzed using DNA sequence analyses. The SRY region, A-oY1 allele was observed in all of the individuals studied. On the other hand,four different alleles corresponding to four Y-chromosome haplotypes were detected at the SRYM18 microsatellite region. Among native Anatolianbreeds (n=143), H6 haplotype (80.41%), H4 haplotype (9.09%), H8 haplotype (8.40%) and H12 haplotype (2.1%) were identified. H6 haplotype wasobserved in all 16 individuals of Ovis gmelinii anatolica. Pairwise FST values based on haplotype frequencies were calculated for domestic sheep, andthe highest FST value was observed between Karagül and Kıvırcık along with Karagül and Ovis gmelinii anatolica with pairwise FST value of 0.43202(P

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