Ivan DINEV, Katerina TODOROVA, Маrin ALEXANDROV, Yulian ANANIEV, Maya GALABOVA, Ismet KALKANOV

Coronavirus Pnömoenteritis Sendromlu Buzağılarda Ultrastrüktürel ve İmmunohistokimyasal İncelemeler

Bu çalışmanın amacı spontan pnömoenteritis sendromlu yeni doğan ve büyüme dönemindeki buzağılarda hastalığın morfogenezine yönelik bazı yapısal özellikleri incelemektir. Çalışma ülkenin 4 bölgesinden 6 çiftlikte toplam 370 adet 24 saatlik ile 25 günlük arasındaki buzağı üzerinde gerçekleştirildi. BoНV-1, BVDV, BRSV ve BPI-3’ün hızlı tespiti amacıyla Multiscreen Ag ELISA Bovine respiratory, Pulmotest respiratory tetra ELISA kit kullanıldı (BIOX Diagnostics, Belçika). Sığır dışkısında Rotavirus, Coronavirus, Escherichia coli F5, Cryptosporidium parvum ve Clostridium perfringens tespiti amacıyla Rainbow calf scour 5 BIO K 306 kullanıldı (BIOX Diagnostics, Belçika). Vakaların %5’inde BoНV-1, BVDV, BRSV ve BPI-3 pnömonili buzağıların akciğer doku lizatlarında birlikte gözlemlendi. Çalışmada kullanılan antijenik, ultrastrüktürel ve virolojik tanı metotlarının başarılı bir şekilde pulmoner ve gastrointestinal viral enfeksiyonların tespitinde kullanılabileceği belirlendi. Akciğer ve barsak dokularında elektron mikroskopi ve immunohistokimyasal metotlar tanıda uygulanabilir olup IBR, BVD, BRSV, Mannheimia haemolytica, C. parvum, BRV ve E. coli K99 (F5) gibi diğer yaygın hastalıklardan ayırıcı tanıda önemlidirler.

Ultrastructural and Immunohistochemical Investigations in Calves with Coronavirus Pneumoenteritis Syndrome

The aim of present studies was the structural and morphogenetic investigation of spontaneous pneumoenteritis syndrome in newborn andgrowing calves with regard to confirmation of some structural features of disease morphogenesis. The research was done with 370 calvesfrom 6 cattle farms in 4 regions of the country, at the age of 24 h - 25 days. For rapid antigenic and viral detection of pathogens, MultiscreenAg ELISA, Bovine respiratory, Pulmotest respiratory tetra ELISA kit for antigenic diagnosis of BoНV-1, BVDV, BRSV, and BPI-3 sandwich test fortissue lysates (BIOX Diagnostics, Belgium) and Rainbow calf scour 5 BIO K 306 Detection of Bovine Rotavirus, Coronavirus, Escherichia coliF5, Cryptosporidium parvum and Clostridium perfringens in bovine stool (BIOX Diagnostics, Belgium) were used. In 5% of cases, laboratoryantigenic tests of lung tissue lysates from pneumonic calves detected co-infections with BoНV-1, BVDV, BRSV and BPI-3. The utilised antigenic,ultrastructural and virological diagnostic methods allowed concluding that they could be successfully used in the diagnostics of pulmonaryand gastrointestinal viral infections in juvenile calves. Electron microscopy and immunohistochemical methods of lung and intestinal tissueare also important and applicable for diagnostics and in differential diagnostic recognition of the condition from other common diseases asIBR, BVD, BRSV, Mannheimia haemolytica, Cryptosporidium parvum, BRV and E. coli K99 (F5).

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