GH-AluI ve MYF5-TaqI Polimorfizmlerinin Erkek Holstein Buzağılarında Sütten Kesim Ağırlığı ve Vücut Ölçüleri Üzerine Etkileri
Canlı ağırlık ve vücut ölçüleri gibi önemli kantitatif özellikler, karkas verimi ve buzağı yaşama gücünü etkilemektedirler. Büyümedeki rolleri nedeniyle büyüme hormonu geni (GH), miyojenik faktör 5 (MYF5), yağ asidi bağlayıcı protein 4 (FABP4) ve sinyal dönüştürücü ve transkripsiyonu aktive edici faktör 5A (STAT5A) canlı ağırlık ve vücut ölçüleri için aday gen olarak önerilmiştir. Bu çalışmada erkek Holstein buzağılarda GHAluI, MYF5-TaqI, FABP4-HinII ve STAT5A-AvaI polimorfizmleri belirlenerek, elde edilen genotip verileriyle canlı ağırlık artışı ve vücut ölçüleri arasındaki ilişkilerin araştırılması amaçlanmıştır. İncelenen örneklerin bu aday genlerdeki SNP markırları yönünden genotipleri restriksiyon parçacık uzunluk polimorfizmi (RFLP) analiziyle belirlenmiştir. İncelenen örneklerde GH için L allel, MYF5 için A allel, FABP4 için G allel ve STAT5A için C allel frekansı diğer allelden yüksek bulunmuştur (sırasıyla 0.96, 0.61, 0.79 ve 0.74). Elde edilen genotip verileri kullanılarak yapılan regresyon analizi sonunda GH-AluI polimorfizmi ile sütten kesimdeki (WW) ve 6. ay canlı ağırlıkları ile tüm ölçüm dönemlerindeki gögüs çevresi uzunluğu ile ilişkili olduğu görülmüştür. MYF5-TaqI polimorfizmi ise doğumdaki vücut uzunluğu ve doğum ağırlığı (BW) ile ilişkili bulunmuştur. İncelenen örneklerde FABP4-HinII polimorfizmi ölçülen özelliklerin hiç biri ile ilişkili bulunmamıştır. Çalışma sonunda GH-AluI ve MYF5-TaqI polimorfizmlerinin canlı ağırlık ve vücut ölçüleri yönünden yapılacak seleksiyon çalışmalarında kullanılabileceği düşünülmüştür.
Effects of GH-AluI and MYF5-TaqI Polymorphisms on Weaning Weight and Body Measurements in Holstein Young Bulls
Live weight and body measurements are economically important quantitative traits that affect carcass yield and calf survival in cattle. Fourgenes, growth hormone gene (GH), myogenic factor 5 (MYF5), fatty acid binding protein 4 (FABP4) and signal transducers and activators oftranscription 5A (STAT5A) were chosen as candidate genes for live weight and body measurements due to their important role in growth. Theaims of this study were to genotype GH-AluI, MYF5-TaqI, FABP4-HinII and STAT5A-AvaI polymorphisms and to investigate their associationswith live weights and body measurements in Holstein young bulls. Genotyping of the single nucleotide polymorphism (SNP) markers inthese candidate genes was carried out using the restriction fragment length polymorphism (RFLP) analysis. Frequencies of L allele for GH, Aallele for MYF5, G allele for FABP4 and C allele for STAT5A were, 0.96, 0.61, 0.79 and 0.74, respectively in the examined animals. The regressionanalysis indicated that the GH-AluI polymorphism showed an association with weaning weight (WW), 180th day weight and hearth girthfrom birth to 180 days of age. The MYF5-TaqI polymorphism was found to influence body length at birth and birth weight (BW). However,no significant association was detected between the FABP4-HinII genotypes and measured traits. The GH-AluI and MYF5-TaqI polymorphismsmay be useful for selection on live weight and body measurement traits in Holstein young bulls.
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