Belma KONUKLUGİL, Hajar HEYDARİ, Bülent GÖZCELİOĞLU

Türkiye’deki Dictyonella incisa’nın In vitro Biyoaktivitesinin Değerlendirilmesi

Deniz kaynaklarının, yapısal olarak eşsiz farmasötik olarak etkili sekonder metabolitleri ürettiği bilinmektedir. Türkiye kıyılarından toplanan süngerler ile devam eden çalışmalarımızesnasında toplanan Dictyonella incisa’nın metanolik ekstraktının antioksidan, sitotoksik ve antimikrobiyal aktivitelerini araştırılmıştır. Antioksidan aktivite süperoksit radikal süpürme testi ile, sitotoksik aktivite HCT-116 hücre hattı ve HEp-2 hücre hattına karşı MTT yöntemi ile, antimikrobiyal aktivite ise bazı Gram pozitif, Gram negatif bakteri ve maya suşlarına karşı test edilmiştir. Ayrıca süngerde bulunan kolesterol ve kolestan NMR yöntemi ile izole edilip yapısı tayin edilmiştir. Bu çalışma, Türkiye kıyılarından toplanan D. incisa’nın biyoaktivite ve ikincil metabolitlerini inceleyen ilk çalışmadır.

In vitro Evaluation of Bioactivity of Dictyonella incisa from Turkey

Marine sources are known to produce structurally unique pharmaceutically potent secondary metabolites. During the course of our studieson Turkish marine sponges, we have collected Dictyonella incisa from Turkey’s coast and methanolic extract was investigated for antioxidant,cytotoxic and antimicrobial activity. Antioxidant activity was evaluated by the superoxide radical scavenging assay, cytotoxic activity wasdetermined against HCT-116 and HEp-2 cell lines by MTT assay. Antimicrobial activity was tested against some Gram positive, Gram negativebacteria and yeasts. Also cholesterol and cholestane isolated and the structure were identified by NMR methods. This is the first work on the bioactivity and secondary metabolites of D. incisa collected from Turkish coasts.

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