Kemal Özdem ÖZTABAK, Cemal UN, Iraz AKIS AKAD, Funda YILDIRIM, Burçin TELLİOĞLU, Merve ÇELİK, Nehir OZDEMIR OZGENTURK, Zehra OMEROGLU ULU, Salih ULU, Aydin GUREL

Terrier Köpeklerinin Normal ve Tümörlü Meme Bezi Dokusunda Eksprese Edilen Genlerin Birincil Analizi

Meme bezi tümörü, dişi köpeklerde en sık rastlanan tümör türüdür. Tümör oluşumu ve tümör oluşum mekanizması ile ilgili genler hakkındaki veriler yetersizdir. Köpekteki tümörigenez çalışmalarının insan meme bezi tümörleri için bir model olabileceğini görmek için karşılaştırmalı çalışmalar yürütülmüştür. Bu çalışmada, sağlıklı bir Terrier köpeğinin normal meme dokusundan ve hasta bir köpekten tümörlü meme dokusundan toplanan iki farklı dokudan iki farklı cDNA kütüphanesi oluşturuldu. Bir köpek meme bezi EST kolleksiyonu geliştirmek için, iki kütüphaneden rastgele seçilen toplam 2304 koloni dizilendi. Ham EST verileri Phred/Phrap programları ile analiz edildi ve okunabilir EST dizileri CAP3 programı ile bir araya getirildi. Tüm EST dizileri 45 kontig ve 2203 singlet grubuna ayrıldı. Tüm benzersiz sekansların varsayımsal fonksiyonları, NCBI BLAST tarafından gen homolojisine dayalı olarak belirlendi ve BLAST2GO ile açıklandı. Bu çalışmanın sonuçları, köpeklerin fonksiyonel genom çalışmaları için çok değerli bir kaynaktır.

Analysis of Expressed Genes in Normal and Tumoral Mammary Gland Tissue of the Terrier Dog

Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanismof tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in the dog couldbe a model for human mammary gland tumors. In this study, we constructed two different cDNA libraries from mammary tissue, whichwere collected from a normal mammary tissue of a healthy Terrier dog and a tumoral mammary tissue of a sick dog. A total 2304 colonieswhich are randomly picked out from the two libraries were sequenced for developing a dog mammary gland ESTs collection. Raw EST datawere analyzed with Phred/Phrap programs and readable EST sequences were assembled with the CAP3 program. All of EST sequences weregrouped into 45 contig and 2203 singletons. Putative functions of all unique sequences were designated by NCBI BLAST based on genehomology and annotated by BLAST2GO. The results of this study are a very valuable resource for functional genome studies of the dogs.

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