Jie SUN, Yourong YE, Herong LIAO, Lei ZHANG, Heng YANG

Effects of Steroid Hormones on Epithelial and Stromal Cells Culture and Proliferation of Laying Hens, In vitro

The aim of this study was to study the effect of steroid hormones on the morphology, growth and proliferation of endometrial cells of laying hens in vitro. Endometrial epithelial and stromal cells were isolated and cultured via type I collagenase digestion, which then were identified by using keratin CK18 or vimentin by immunohistochemistry and treated with steroid hormones, such as E2 and P4, and the growth curves in vitro evaluated by MTT assay. The results showed that endometrial epithelial and stromal cells were successfully isolated and cultured, both of which began to enter the logarithmic phase of growth after 24 h and reached the plateau phase at 7 days after inoculation. Compared to control group, the endometrial epithelial cells were significantly proliferated (P

Steroid Hormonların Yumurtacı Tavukların Epitel ve Stroma Hücre Kültürüne İn vitro Etkileri

Bu çalışmanın amacı, steroid hormonların in vitro yumurtacı tavukların endometriyal hücrelerinin morfoloji, büyüme ve proliferasyonu üzerine etkilerini araştırmaktır. Endometriyal epitel ve stroma hücreleri izole edildi ve tip I kollagenaz digesyon ile kültüre edildi ve sonrasında immunohistokimyasal olarak keratin CK18 veya vimentin ile tanımlandı. E2 ve P4 gibi steroid hormonlar ile muamele edildikten sonra MTT testi ile in vitro büyüme eğrisi değerlendirildi. Elde edilen sonuçlar endometriyal epitelyum ve stroma hücrelerinin başarılı bir şekilde izole ve kültüre edildiğini ve her ikisinin de 24 saat sonrası büyümenin logaritmik fazına girmeye başladıklarını ve inokulasyon sonrası 7 günde plato fazına eriştiklerini gösterdi. Kontrol grubu ile karşılaştırıldığında E2 (100 nmol/L) ve P4 (100 nmol/L) ile muamele edilen endometriyal hücrelerin anlamlı derece prolifere oldukları (P

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