Maryam ROYAN, Hossein ALAIE KORDGHASHLAGHI, Fazlullah AFRAZ, Maryam HASHEMI, Seyed Mohammad Farhad VAHIDI, Ramin SEIGHALANI

Kuzey İran Köy Tavuklarında Salmonella Enteritidis ve Salmonella Typhimurium’a Karşı Biyokontrol Stratejisi Olarak Lactobacilli İzolatlarının Taranması

Bu çalışmada, İran’daki köy tavuklarının barsaklarından Gram pozitif ve katalaz negatif çomak şekilli bakteriler izole edildi. Asit ve safra toleransı için bir ön taramanın ardından 14 Lactobacillus reuteri izolatı antibiyotik direnci ile Salmonella Enteritidis ve Salmonella Typhimurium’a karşı antimikrobiyal aktivite için beta hemolizis şekillenmemesine göre seçildi. 14 izolatın tümü 3 saat süreyle pH 3’ü tolere edebildi ve %0.3 safra tuzlarına karşı direnci %50’den fazla idi. Bu 14 izolat içinde, L. reuteri ABRIG22, L. reuteri ABRIG3, L. reuteri ABRIG18, L. reuteri ABRIG17, L. reuteri ABRIG8 ve L. reuteri ABRIG9 2 saat süreyle pH 2.3’e dayanabildi. L. reuteri ABRIG17 3 saat süreyle pH 2.3’te hayatta kalabilen tek izolattı. Beş izolat daha sert asidik ortama dayanabildi. Bunlar ayrıca daha az supernatant ile Salmonella Enteritidis ve Salmonella Typhimurium’u %90’dan daha fazla inhibe edebildi. Bu çalışmanın sonuçları, Kuzay İran’da yerel tavukların barsaklarından izole edilen L. reuteri’nin, S. Enteritidis ve S. Typhimurium enfeksiyonlarında potansiyel antimikrobiyal probiyotikler olarak kullanılabileceğini göstermektedir.

Screening Lactobacilli Isolates from Northern Iran Backyard Chickens as Bio-control Strategy Against Salmonella Enteritidis and Salmonella Typhimurium

In this study, Gram positive and catalase negative rod-shaped bacterial strains were isolated from Iran’s backyard chicken intestines. After apreliminary screening for acid and bile tolerance, 14 Lactobacillus reuteri isolates were selected based on susceptibility to the antibiotics andthe absence of beta haemolysis for antimicrobial activity against Salmonella Enteritidis and Salmonella Typhimurium. All 14 isolates wereable to tolerate pH 3 for 3 h and their resistance to 0.3% bile salts was more than 50%. Among these 14 isolates, L. reuteri ABRIG22, L. reuteriABRIG3, L. reuteri ABRIG18, L. reuteri ABRIG17, L. reuteri ABRIG8 and L. reuteri ABRIG9 were able to withstand pH 2.3 for 2 h, and L. reuteri ABRIG17was the only isolate with good survivability in pH 2.3 for 3 h. Five isolates were able to withstand stronger acidic conditions and inhibitSalmonella Enteritidis and Salmonella Typhimurium by more than 90% with less of their supernatants. The results of this study demonstratedthat isolated L. reuteri from intestines of chickens native to Northern Iran could be introduced as potential antimicrobial probiotic strains tocontrol S. Enteritidis and S. Typhimurium infection.

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