The research of clonal relationship among Aeromonas strains isolated from human, animal and drinking water by PFGE
Aeromonaslar insanlarda çeşitli enfeksiyonlara neden olmakta, içme suları ile insanlara bulaşabilmekte ve hayvanlardaki varlığı, insanlar için potansiyel risk olarak değerlendirilmektedir. Bu çalışmayla pulsed-field gel electrophoresis (PFGE) ile diyareli insanlardan (14 suş), sağlıklı gıda işçilerinden (2 suş), hayvanlardan (24 suş) ve içme sularından (12 suş) izole edilen 52 Aeromonas suşu arasındaki klonal ilişkinin araştırılması amaçlandı. Bir diyareli insan izolatı ve bir sığır izolatı arasında klonal ilişki tespit edildi. İnsan ve su izolatları arasında klonal ilişki tespit edilmedi. İki balık izolatı arasında (A. caviae ve A. sobria) ayırtedilemez PFGE paterni tespit edildi. Sonuç olarak, klonal ilişkili suşlar tespit edilmesine rağmen baskın bir klon tespit edilmedi. Özellikle hayvanların, insan Aeromonas infeksiyonları yönünden epidemiyolojik önemini aydınlatmaya, ayrıca çevresel izolatların identifikasyonuna yönelik kapsamlı çalışmalara ihtiyaç olduğu görüldü.
PFGE ile insan, hayvan ve içme sularından izole edilen Aeromonas suşları arasında klonal ilişkinin araştırılması
Aeromonads infect human through potable water and causes various infections. Their existence in animal are being assessed as potential risk for human health. The aim of this study was to investigate clonal relationship among 52 Aeromonas strains isolated from human with diarrhea (14 strains), healthy food workers (2 strains), animals (24 strains) and drinking water (12 strains) by pulsed-field gel electrophoresis (PFGE). Clonal relation was determined between one diarrheic human isolate and one cattle isolate. No clonal relation was determined between drinking water and human isolates. Two fish isolates, A. caviae and A. sobria, were not distinguished PFGE patterns. Consequently no predominant clone was determined while clonal related strains were determined. Particularly, it is necessary to elicit the epidemiological importance of animals in respect of human Aeromonas infections and extensive studies are required for identification of environmental isolates.
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