In vitro antimutagenicity of Allium Tuncelianum ethanol extract against induction of chromosome aberration by mutagenic agent Mitomycine C
Allium Tuncelianum, Tunceli/Türkiye’de lokalize olmuş bir sarımsak türüdür. Sarımsak bitkisi organosülfür bileşikler adı verilen, antikarsinojenik ve antimutajenik etkileri olduğu daha önceden bildirilmiş olan biyolojik yönden aktif komponentler içermektedir. Bu çalışmada A. Tuncelianum ethanolik ekstraktının in vitro antigenotoksik aktivitesi, kromozom aberrasyon testi ile insan lenfosit hücre kültüründe çalışılmıştır. A. Tuncelianum ekstraktı etanol ile soxhlet kullanılarak elde edilmiştir. Bu araştırmada A. Tuncelianum’un etanolik ekstraktı insan lenfosit hücrelerinde kromozom aberrasyon testi ile antigenotoksik aktivitesi çalışılmıştır. A. Tuncelianum’un etanolik ekstraktı, 0.10, 0.15, 0.20 ve 0.25 μl/mL konsantrasyonlarda aseton (negative kontrol) ve Mitomisin C (pozitif kontrol)’ye karşı kromatid ve kromozom kırıkları, kromozom değişimi, kromatid birleşimi ve poliploidi bakımından taranmıştır. Uygulamadan 24 saat sonra, kromozomal aberrasyon frekansı bakımından A. Tuncelianum’un etanolik ekstraktı tüm konsantrasyonlarda önemli bir düşüş ortaya koymuştur. Sonuç olarak A. Tuncelianum’un etanolik ekstraktı MMC uygulanan kültür hücreleri ile karşılaştırıldığında kromozomal aberrasyonda önemli bir düşüşe neden olmuştur. Bu sonuçlara gore A. Tuncelianum’un mutajenite ve karsinojeniteye karşı koruyucu gıda olarak diyetle tüketilebileceği düşünülmektedir.
Mutajenik ajan mitomisin C ile uyarılmış kromozom aberrasyona karşı allium tuncelianum etanolik ekstraktının in vitro antimutajenik etkinliği
Allium Tuncelianum is a garlic species which is locally produced in Tunceli, Turkey. Garlic contains several biologically active compounds known as organosulfur compounds which are reported to have anticarcinogenic and antimutagenic effects. Present study examined the in vitro antigenotoxic activity of ethanolic extracts of A. Tuncelianum in human lymphocyte cells by chromosome aberration test. Samples of A. Tuncelianum were extracted by ethanol using soxhlet extractor. The extract was tested on human lymphocyte cell culture in vitro chromosome aberration test. The effect of ethanol extract of A. Tuncelianum at concentrations of 0.10, 0.15, 0.20, 0.25 μl/mL were screened for chromatid and chromosome breaks, chromosome exchange as well as chromatid union and polyploid cells against negative acetone and positive control Mitomycine C. A significant decrease in the frequency of chromosomal aberration was observed for all treatments with A. Tuncelianum ethanol extract at 24 h. In conclusion, ethanol extract of A. tuncelianum significantly reduced the chromosomal aberration rate as compared with the culture treated with MMC. The results could also indicate that A. Tuncelianum could be protective against mutagenic and carcinogenic compounds when consumed through the diet.
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- 1. Belloir C, Singh V, Daurat C, Siess MH, Le Bon AM: Protective effects of garlic sulfur compounds against DNA damage induced by direct- and indirect-acting genotoxic agents in HepG2 cells. Food Chem Toxicol, 44, 827-834, 2006.
- 2. Bhuvaneswari V, Phil M, Abraham SK, Nagini S: Combinatorial antigenotoxic and anticarcinogenic effects of tomato and garlic through modulation of xenobiotic-metabolizing enzymes during hamster buccal pouch carcinogenesis. Nutrition, 21, 726-731, 2005.
- 3. Shukla Y, Karla N: Cancer chemoprevention with garlic and its constituents. Cancer Lett, 247, 167-181, 2007.
- 4. Bhattacharya K, Yadava S, Papp T, Schiffmann D, Rahman Q: Reduction of chrysotile asbestos-induced genotoxicity in human peripheral blood lymphocytes by garlic extract. Toxicol Lett, 153, 327-332, 2004.
- 5. Assayed ME, Khalaf AA, Salem HA: Protective effects of garlic extract and vitamin C against in vivo cypermethrin-induced teratogenic effects in rat offspring. Food Chem Toxicol, 48, 3153-3158, 2010.
- 6. Ipek M, Ipek A, Simon PW: Genetic characterization of Allium tuncelianum: An endemic edible Allium species with garlic odor. Sci Hortic-Amsterdam, 115 (4): 409-415, 2008.
- 7. Ozkan O, Aydin H, Bagcigil AF: In vitro evaluation of antimicrobial activities of Salvia verticillata and Phlomis pungens. Kafkas Univ Vet Fak Derg, 15 (4): 587-590, 2009.
- 8. Choudhury AR, Das T, Sharma A: Mustard oil and garlic extract as inhibitors of sodium arsenite-induced chromosomal breaks in vivo. Cancer Lett, 121, 45-52, 1997.
- 9. Shukla Y, Taneja P: Antimutagenic effects of garlic extract on chromosomal aberrations. Cancer Lett, 176, 31-36, 2002.
- 10. Monobe M, Arimoto-Kobayashi S, Abramov KA: Pseudouridine, a beer component, reduces radiation-induced chromosome aberrations in human lymphocytes. Mutat Res, 538, 93-99, 2003.
- 11. Jabar MA, Al-Mossavi A: Susceptibility of some multiple resistant bacteria to garlic extract. Afr J Biotech, 6 (6): 771-776, 2007.
- 12. Gul S, Savsar A, Tayfa Z: Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro. Cytotechnology 59, 113-119, 2009.
- 13. Assayed ME, Khalaf AA, Salem HA: Protective effects of garlic extract and vitamin C against in vivo cypermethrin-induced cytogenetic damage in rat bone-marrow. Mutat Res, 702, 1-7, 2010.
- 14. Singh BN, Singh BR, Singh RL, Prakash D, Singh DP, Sarma BK, Upadhyay G, Singh HB: Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. Food Chem Toxicol, 47, 1161-1167, 2009.
- 15. Herman-Antosiewicz A, Singh SV: Signal transduction pathways leading to cell cycle arrest and apoptosis induction in cancer cells by Allium vegetable-derived organosulfur compounds: A review. Mutat Res, 555, 121-131, 2004.