SÜLEYMAN KÖK, SERTAÇ ATALAY, Mustafa SAVAŞÇI, Hasan Semih EKEN

Characterization of calpastatin gene in purebred and crossbred Turkish Grey steppe cattle

Kalpastatin (CAST), memeli dokularında bulunan ve kalsiyuma bağımlı doğal bir proteaz olan μ-kalpain’in spesifik bir inhibitörüdür. Sığır CAST geni içindeki genetik varyantlar, polimeraz zincir reaksiyonu-restriksiyon parça uzunluk polimorfizmi (PCR-RFLP) metodu kullanılarak analiz edildi. Çalışmada hayvan materyali olarak, Boz ırk ve Boz ırk x İsviçre Esmer melezlerinden oluşan 132 boğa ve düve kullanıldı. Çalışılan hayvan örneklerinde CAST/RsaI polimorfizminin C ve G alleleri karakterize edildi. C allel frekansı, saf Türk Boz ırklara nazaran melez grup içinde önemli bulunmuştur (P

Anahtar Kelimeler:

düve, Türkiye, kısıtlı fragment uzunluğu polimorfizmi, proteinaz, polimeraz zincir reaksiyonu, yerli çiftlik hayvanları, genotip, genetik çeşitlilik, genetik değişim, genetik polimorfizm, genetik kararlılık, genler, gen frekansı, gen ifadesi, melez ırklar, sığır ırkları, kalsiyum, boğa, Amerikan Esmer Irkı, alleller, sığır

Safkan ve melez Türk Boz ırk sığırlarda kalpastatin geninin karakterizasyonu

Calpastatin (CAST) is a specific inhibitor of calcium-dependent neutral protease μ-calpain found in mammalian tissues. The genetic variants in the bovine CAST gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The animal material of the study consisted of 132 bulls and heifers in Turkish Grey steppe cattle and Turkish Grey steppe x Brown Swiss crossbreds. C and G alleles are characterized by CAST/RsaI polymorphism were detected in the sample of animals were studied. Allele frequency C was significantly frequent in the crossbred group compared to the pure Turkish Grey steppe animals (P<0.05). In the total samples of animals, the average allele frequency C was 56.1%. Genotypic frequencies were estimated as 0.257, 0.499 and 0.243 in the purebred Turkish Grey steppe, and 0.388, 0.470 and 0.142 in the Turkish Grey steppe x Brown Swiss crossbred population for genotypes CC, CG and GG, respectively. As a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.

Keywords:

heifers, Turkey, restriction fragment length polymorphism, proteinases, polymerase chain reaction, native livestock, genotypes, genetic variation, genetic variance, genetic polymorphism, genetic equilibrium, genes, gene frequency, gene expression, crossbreds, cattle breeds, calcium, bulls, American Brown Swiss, alleles, cattle,

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