Görkem KISMALI, Merve ALPAY, SERKAN SAYINER, Deniz TURAN, Ali Burak BALKAN, BERRİN SALMANOĞLU, Hilal KARAGÜL, TEVHİDE SEL, BURCU MENEKŞE BALKAN

The Effects of Vitamin E on Antioxidant Enzyme Activity in HepG2 Cells [1]

Güçlü bir antioksidan olan vitamin E'nin (alfa-tokoferolsüksinat) HepG2 hücrelerinde antioksidan enzim aktiviteleri üzerine etkisinin araştırılması amaçlanmıştır. Çalışma materyali olarak HepG2 hücre hattı kullanılmıştır. Vitamin E uygulanan hücreler çalışma grubunu vitamin E uygulaması yapılmayan hücreler kontrol grubunu oluşturmuştur. Çalışma grubu hücrelerine 10 µM, 50 µM, 50 mM dozlarda vitamin E uygulaması yapılarak, 24 saat sonunda HepG2 hücrelerinde antioksidan enzimlerden katalaz (CAT), süperoksitdismutaz (SOD) ve glutasyonperoksidaz (GPx) aktiviteleri spektrofotometrik olarak ölçülmüştür. 50 mM vitamin E uygulanan HepG2 hücrelerinde GPx enzim aktivitesinde anlamlı bir artış saptanmıştır. Ancak, 10 µM ve 50 µM vitamin E uygulanan HepG2 hücrelerinde ise anlamlı bir azalma meydana gelmiştir. Vitamin E uygulaması yapılan hücrelerdeki SOD aktivitesi vitamin E uygulaması yapılmayan kontrol grubuna göre daha düşük ölçülürken, 50 µM vitamin E uygulanan HepG2 hücrelerinde SOD aktivitesinde anlamlı azalma tespit edilmiştir. 50 µM vitamin E uygulanan HepG2 hücrelerindeki CAT enzim aktivitesi kontrol grubuna göre daha yüksek bulunurken, 10 ?M ve 50 mM vitamin E uygulanan hücrelerdeki CAT enzim aktivitesi kontrol grubuna göre daha düşük tespit edilmiştir. Vitamin E'nin HepG2 hücrelerinde antioksidan enzim aktiviteleri üzerinde doz-bağımlı etkisinin olduğu belirlenmiştir

Vitamin E'nin HepG2 Hücrelerinde Antioksidan Enzim AktivitesiÜzerine Etkileri

It is aimed to investigate the effect of vitamin E, powerful antioxidant (alpha-tocopherol succinate) on antioxidant enzyme activities in hepatocellular carcinoma (HepG2) cells. The hepatocellular carcinoma cell line HepG2 was used and the cells were cultured in the absence (control) or presence of different dose of vitamin E (50 mM, 50 ?M and 10 ?M vitamin E) for 24 h. The effect of vitamin E (alpha-tocopherol succinate) on catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities in hepatocarcinoma cells were measured by spectrophotometry. A significant decrease in GPx activity was detected in 50 mM vitamin E treated HepG2 cells. However a significant decrease occurred in 10 µM and 50µM vitamin E applied HepG2 cells. SOD activity in study groups were lower than in control cells. In addition to this, the decrease in SOD activity in 50 mM vitamin E applied cells was significant. CAT enzyme activity in 50 ?m vitamin E applied HepG2 cells was higher and, in 10 ?M and 50 mM vitamin E applied HepG2 cells were lower than in control group. It was determined that vitamin E has a dose-dependent effect on antioxidant enzyme activity in HepG2 cells

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