Abdolhadi RASTAD, Ali Asghar SADEGHI, Mohammad CHAMANI, Parvin SHAWRANG

Abdolhadi RASTAD 1 Ali Asghar SADEGHI 1? Mohammad CHAMANI 1 Parvin SHAWRANG 2

Bu çalışma, farklı dozlardaki timokuinon (TQ) kullanımının oksidatif strese maruz bırakılan broiler tavuklardaki performans, antioksidan kapasite ve kan biyokimyasal parametreler üzerine etkilerini araştırmak amacıyla yürütüldü. Toplam 320 adet bir günlük broiler civcivler rastgele düzen sistemi içerisinde gruplandırıldı. Civcivler iki gruba ayrılarak 160 civciv haftada üç kez ve 3 haftalıktan itibaren olmak üzere tert-BHP enjeksiyonu (ip. olarak 0.02 mmol her bir kg vücut ağırlık) ile oksidatif strese maruz bırakıldı. Diğer 160 civcive stres uygulanmadı. Her bir grup için timokuinon enjeksiyonu ip. olarak 4 doz halinde (her bir kg vücut ağırlığa 0, 5, 8 ve 11 mg dozlarında - her bir doz için 40 civciv olacak şekilde) haftada üç kez ve 2 haftalıktan itibaren olmak üzere uygulandı. Elde edilen sonuçlar her bir vücut ağırlığa 8 mg dozundaki TQ uygulamasının total vücut ağırlık kazanımını ve yem tüketimini arttırırken her bir vücut ağırlığa 11 mg dozundaki TQ uygulamasının ise yem dönüşüm oranını stres oluşturulmayan civcivlerde geliştirdiğini gösterdi (P

Effects of Thymoquinone on Cortisol Level, Blood Antioxidant Parameters and Capacity in Broiler Chickens under Oxidative Stress

This study was conducted to evaluate the effect of graded doses of thymoquinone (TQ) in broiler chickens exposed to oxidative stress on performance, antioxidant capacity and blood biochemical parameters. A total of 320 one-day-old broiler chicks was used in a completely randomized design. Chicks were divided to two groups, 160 chicks exposed to oxidative stress induced by tertBHP injection (ip, 0.02 mmol per kg body weight) three in week from week 3 of age and 160 chicks as non-stressed. In each group, thymoquinone injection (ip) at 4 doses of 0, 5, 8 and 11 mg per kg body weight (40 chicks for each dose) were done three in week from week 2 of age. Results showed that TQ at dose of 8 mg per kg body weight increased total body gain and feed intake, but at dose of 11 mg per kg body weight improved feed conversion ratio in non-stressed chickens (P<0.05). Administration of TQ significantly increased the enzyme levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and the levels of total antioxidant capacity (TAC), and decreased cortisol, enzyme activities of alanine aminotransferase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) and malondialdehyde (MDA) in both non-stressed and stressed chickens. Based on the results of this study, thymoquinone administration at dose of 8 and 11 mg/kg body weight could ameliorate the adverse effect of oxidative stress on growth and enhance the blood antioxidant capacity and antioxidant enzyme activity.

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