Sema USLU, MECİT YÖRÜK, LEYLA MİS, BAHAT COMBA

Localisation of Estrogen Receptor Alpha and Progesterone Receptor B in Goat Ovaries During Breeding and Non-Breeding Season [1] [2]

Sunulan bu çalışmada, üreme sezonu içinde ve dışındaki keçi ovaryumlarında, östrojen reseptör alfa (ER-?) ve progesteron reseptör B'nin (PRB) yerleşim yerlerinin immünohistokimyasal olarak araştırılması amaçlandı. Mezbaha şartlarında kesilen dişi keçilerden, üreme sezonu içinde (n=10) ve dışında (n=10) elde edilen ovaryumlar kullanıldı. Aynı hayvanların, serum steroid hormon düzeylerini (E2, P4) belirlemek için kan örnekleri alındı. ER-? ve PR-B'nin ovaryumlardaki yerleşimleri immünohistokimyasal olarak ABC metodu ile belirlendi. Üreme sezonu içinde, ER-? reseptörleri germinatif epitelyum ve foliküllerin granüloza hücrelerinde saptandı. PR-B reseptörlerinin ise corpus luteum (CL) hücrelerinde yoğunlaştığı belirlendi. Aynı reseptörlerin, preovulator foliküllerin teka eksterna hücrelerinde de zayıf olarak bulundukları gözlendi. Üreme sezonu dışında, ER-? reseptörleri germinatif epitelyum ve bazı kan damarlarının düz kas hücrelerinde zayıf pozitif reaksiyon gösterdi. PR-B ise germinatif epitelyum ve bazı stroma hücrelerinde pozitif boyandı. Keçilerde, üreme sezonu içi- (E2: 11.83±1.70 pg/mL, P4: 10.08±1.58 ng/mL) ve dışındaki (E2: 2.33±0.85 pg/ml, P4: 0.21±0.04 ng/ml) ortalama steroid hormon profillerinin bu reseptörlerin ovaryumlardaki lokalizasyon yoğunlukları ile uyumlu olduğu kanısına varıldı.

Keçi Ovaryumunda Üreme Sezonu İçinde ve Dışında Östrojen Reseptör alfa ve Progesteron Reseptör B'nin Lokalizasyonu

The main objective of this study was to investigate the localisation of estrogen receptor alpha (ER-?) and progesterone receptor B (PR-B) by immunohistochemistry in goat ovaries during in- and out of breeding season. The ovaries obtained from goats slaughtered in breeding season (n=10) and non-breeding season (n=10) were used. From the same animals, blood samples were taken to determine the levels of serum steroid hormones (E2, P4). The ER-? and PR-B immunohistochemical distributions within the ovaries were determined by the ABC method. In breeding season, the ER-? was detected in the germinal epithelium and follicular granulosa cells. The PR-B was determined to concentrate on the corpus luteum (CL) cells. The same receptors were also found to be weak in the theca externa cells of preovulatory follicles. In non-breeding season, the ER-? germinal epithelium and smooth muscle cells of certain blood vessels showed a weak positive reaction. The PR-B was positively stained in the germinal epithelium and few stroma cells. It was suggested that the average steroid hormone profiles in breeding (E2: 11.83±1.70 pg/mL, P4: 10.08±1.58 ng/ml) and non-breeding season (E2 2.33±0.85 pg/ml, P4 0.21±0.04 ng/ml) correlated well with the localisation intensity of receptors in goat ovaries.

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