SERAP KORAL TAŞÇI, TURGAY DEPREM, SEYİT ALİ BİNGÖL, Sevda YILDIZ ELİŞ, ŞAHİN ASLAN

Effect of Melatonin on Catalase Enzyme in Mouse Kidney Tissue

Bu çalışmada, melatonin uygulamasının böbrek dokusunda katalaz üzerine etkisinin immunohistokimyasal olarak araştırılması amaçlandı. Çalışmamızda, 18 adet erkek Swiss albino fare kullanıldı ve bunlar kontrol (n=6), sham (n=6) ve deneme (n=6) olarak üç gruba ayrıldı. Deneme grubuna 28 gün 10 mg/kg dozda melatonin uygulandı (i.p.). Alınan doku örnekleri parafinde bloklandı. Katalaz immunoreaktivitesini incelemek için doku kesitlerine avidin-biotin-peroksidaz kompleks (ABC) tekniği uygulandı. Yapılan incelemeler sonucunda immunohistokimyasal boyamada, üç grupta da reaksiyonun tubulus proksimalislerde olduğu belirlendi. Korteksin iç kısmındaki tubulus proksimalislerde reaksiyonun daha yoğun olduğu görüldü. Tubulus distalislerde, Malpighi cisimciklerinde, damar endotellerinde ve medullada bulunan yapılarda immunoreaktiviteye rastlanmadı. Katalaz immunoreaktivitesinin deneme grubunda, kontrol ve sham grubuna kıyasla daha yoğun olduğu belirlendi. Çalışmamız, melatoninin böbrek dokusu üzerine doğrudan etkisinin olabileceği ve antioksidan mekanizmayı güçlendirerek oksidatif stres durumlarında antioksidan bir terapötik madde olarak kullanılabileceği düşüncelerini desteklemektedir.

Melatoninin Fare Böbrek Dokusunda Katalaz Enzimi Üzerine Etkisi

In this study, we aimed to immunohistochemically investigate the effects of melatonin administration on catalase in the kidney tissue. In our study, 18 male Swiss albino mice were used and they were divided into three groups as control (n=6), sham (n=6) and experimental (n=6). The experimental group received 10 mg/kg dose of melatonin for 28 days (i.p.). Tissue samples were embedded in paraffin. To examine catalase immunoreactivity, avidin-biotin-peroxidase complex (ABC) technique was performed to tissue sections. Based on the results of the immunohistochemical staining, reaction was showed in proximal tubules in all three groups. The reaction in inner cortical proximal tubules was found to be more intense. There was no immunoreactivity in distal tubules, Malpighian bodies, vascular endothelium and structures in the medulla. Catalase immunoreactivity was determined to be more intense in the experimental group than other groups. Our study supports the idea that melatonin could have a direct effect on the kidney tissue and that it could be used as an antioxidant therapeutic agent by strengthening antioxidant mechanism in oxidative stress.

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