Gökhan OTO, Vedat SAĞMANLIGİL, Hasan UYAR, Yıldıray BAŞBUĞAN, Hülya ÖZDEMİR, Özlem Ergül ERKEÇ

Sıçanlarda Pentilentetrazol İle İndüklenen Akut ve Kronik Epilepsi Modellerinde Thymus vulgaris L.’nin Etkileri

Bu çalışmanın amacı pentilentetrazol ile indüklenen akut ve kronik epilepsi modellerinde Thymus vulgaris L. (TVL)’in oksidatif stres, motor koordinasyon ve öğrenme/bellek üzerine etkilerini araştırmaktır. Bu amaçla 64 adet Wistar albino sıçan randomize olarak sekiz gruba ayrıldı. (1) akut kontrol (AC), (2) akut pentilentetrazol (APTZ) (3) akut PTZ + sodyum valproat (APTZ+VPA), (4) akut PTZ +TVL (APTZ+TVL), (5) kronik kontrol (CC), (6) PTZ tutuşma (PTZk), (7) PTZ tutuşma + VPA (PTZk+VPA) ve (8) PTZ tutuşma + TVL (PTZk + TVL). PTZ enjeksiyonundan sonra 30 dk boyunca nöbetler gözlenerek skorlandı. Çalışmada tek doz PTZ (60 mg/kg, ip) enjeksiyonu ile akut PTZ indüklü nöbet modeli oluşturuldu. Tutuşma ise gün aşırı subkonvulsan dozda PTZ (35 mg/kg, ip) uygulanarak toplam 14 enjeksiyonla oluşturuldu. PTZk+VPA, PTZk ve PTZk+TVL grupları arasında nöbet skorları bakımından anlamlı bir fark bulunamadı. Kronik çalışma gruplarında Morris water maze cihazı ile yapılan öğrenme ve bellek testleri yanında rota rod cihazı ile yapılan motor aktivite testlerinde gruplar arasında farklılık görülmedi (P>0.05). Total antioksidan statüsü (TAS), total oksidan statüsü (TOS) ve oksidatif stres indeksi (OSİ) sonuçları, PTZ uygulaması neticesinde antioksidan kapasitenin azaldığı, oksidan kapasitenin ise arttığı belirlendi. T. vulgaris’in özellikle uzun süre uygulanması ile total oksidanantioksidan dengenin sağlandığı görüldü. Sonuç olarak gelecekte, daha yüksek dozlarda T. vulgaris L.’nin PTZ indüklü nöbet skorlarını değiştirip değiştirmeyeceğini belirlenmesi için ek çalışmaların yapılabileceği düşünülmektedir.

Effects of Thymus vulgaris L. in Acute and Chronic Epilepsy Models in Rats Induced by Pentylenetetrazole

The aim of this study was to investigate the effects of Thymus vulgaris L. (TVL) on oxidative stress, motor coordination and learning/memory in acute and chronic epilepsy models in rats induced by Pentylenetetrazole (PTZ). To this end, 64 male Wistar-albino rats were randomly divided into eight groups with 8 rats each: (1) acute control (AC), (2) acute PTZ (APTZ), (3) acute PTZ + sodium valproate (APTZ+VPA), (4) acute PTZ + TVL (APTZ+TVL), (5) chronic control (CC), (6) PTZ kindling (PTZk), (7) PTZ kindling + VPA (PTZk+VPA) and (8) PTZ kindling + TVL (PTZk+TVL) . Seizures were observed for 30 min after each PTZ injection and were scored. Acute PTZ-induced seizures were created by injecting a single convulsive dose of PTZ (60 mg/kg, ip) in acute groups. PTZ kindling was produced by injecting a subconvulsant dose of PTZ (35 mg/kg, ip) every other day, with 14 injections in total. No significant difference was found among the PTZk + VPA, PTZk, and PTZk + TVL groups with regard to seizure scores. No significant difference was found among all the 8 groups in the learning/memory tests conducted using the Morris Water Maze (MWM) test and the motor activity tests conducted using the rotarod test (P>0.05). The analysis of total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) indicated that the administration of PTZ decreased the antioxidant capacity and increased the oxidant capacity. Moreover, the TVL administration established the oxidant/antioxidant balance, particularly in the chronic groups. Further studies are needed to investigate whether high doses of TVL have an effect on PTZ-induced seizure scores.

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