Yong-Hong LIU, Kai-Rui LI, Bo HE, Fei LI, Lu-Yao ZHANG, Jiao-Jiao PAN, Qiang-Rong WANG, Li ZHAO

Preliminary Study of High Efficiency Vaccine of Rhipicephalus (Boophilus) microplus in South Xinjiang, China

The direct damage and spread of pathogens of Rhipicephalus (Boophilus) microplus to cattle -based livestock is extremely serious, the traditional chemical acaricides control have many disadvantages, and vaccine prevention and control is a potential alternative. The commercially available vaccine, based on Rhipicephalus (Boophilus) microplus Bm86, has been favored by farmers and veterinarians in some areas, but it also has drawbacks such as reduced effectiveness due to genetic variation. Based on the fact that Bm91 and Bm86 sharing can enhance the immune effect of Bm86, as well as the characteristics of DNA vaccines, the combination of DNA vaccine and protein vaccine enhanced the immune effect. In this study, adopts the local Rhipicephalus (Boophilus) microplus strains, using prokaryotic expression system preparation Bm86 and Bm91 proteins, and using the eukaryotic expression vector pVAXl containing the CpG sequences constructed Bm86 and Bm91 double gene eukaryotic expression system. This research provides materials for the next step of Co-immunizing animals with Bm86 and Bm91 mixed proteins with Bm86 and Bm91 dual-gene carriers, it also provides a way for co-immunization with multi-antigen protein and multi-gene carriers to control ticks.

Çin’in Güney Xinjiang Bölgesinde Rhipicephalus (Boophilus) microplus’a Karşı Yüksek Verimli Aşı İçin Ön Çalışma

Sığır besiciliğinde Rhipicephalus (Boophilus) microplus’un doğrudan oluşturduğu hasar ve yayılımı oldukça ciddi bir problem olup, geleneksel kimyasal akarisitlerin kontrolde pek çok dezavantajları vardır ve aşı ile koruma ve kontrol alternatif olabilir. Rhipicephalus (Boophilus) microplus Bm86 temelli hazırlanan ve ticari olarak mevcut olan aşı bazı bölgelerde yetiştiriciler ve veteriner hekimler tarafından tercih edilmektedir, ancak genetik varyasyondan dolayı azalmış etkisi gibi negatif tarafları bulunmaktadır. Bm91 ve Bm86’nin birlikte bulunması Bm86’nın bağışıklık oluşturma etkisini artırabileceğinden aynı zamanda DNA aşıların özelliklerinden dolayı DNA aşısının protein aşı ile kombinasyonu bağışıklık etkisini artırabilir. Bu çalışmada yerel Rhipicephalus (Boophilus) microplus türü ile Bm86 ve Bm91 proteinlerinin prokaryotik ekspresyon sistemi ve CpG sekansı içeren pVAXI prokaryotik ekspresyon vektörü aracılı Bm86 ve Bm91 çift gen ökaryotik ekspresyon sistemi kullanıldı. Bu çalışma, Bm86 ve Bm91 proteinleri karışımı ile Bm86 ve Bm91 çift gen taşıyan aşı materyali oluşturmuş, böylece kene kontrolünde çoklu antijen protein ve çoklu gen taşıyan aşı ile koimmunizasyon için bir araç oluşturmuştur.

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