Sarcoptic mange, a notable parasitic disease, causes dermatological alterations among ruminants. Thiol-disulphite hemostasis is a novel oxidative stress parameter. The aim of this study was to evaluate dynamic thiol/disulfide homeostasis in sheep with sarcoptic mange. Total of thirty-six sheep (n=15 female, n=21 male) with sarcoptic mange (Group I), and twelve healthy sheep (Group II) were used in the study. A novel method was used to determine the thiol disulphide parameters. Native thiol, total thiol and Disulphide values were statistically lower in Group I. Disulphide/native thiol, Disulphide/total thiol, and Native thiol/ total thiol proportions had no statistical differences in groups. Sarcoptic mange was probably affected by the thiol Disulphide hemostasis in infected sheep. Thus, the data obtained in this study might form base for further studies to include antioxidant molecules in the treatment protocols
Önemli paraziter bir hastalık olan sarkoptik uyuz ruminantlarda dermatolojik lezyonlara neden olmaktadır. Tiyol-disülfit dengesi yeni bir stress oksidatif parametresidir. Bu çalışmanın amacı sarkoptik uyuzlu koyunlarda dinamik tiyol/disülfit dengesinin değerlendirilmesidir. Bu amaçla sarkoptik uyuzla (grup I) enfekte 36 koyun (n=15 dişi, n=21 erkek) ve 12 sağlıklı koyun (grup II) çalışmaya dahil edilmiştir. Tiyol disülfitin belirlenmesinde yeni bir metot kullanılmıştır. Natif tiyol, total tiyol ve disülfit parametreleri grup I’ de istatistiksel olarak düşük seyrederken disülfit/natif tiyol, disülfit/total tiyol ve natif tiyol/total tiyol oranları arasında gruplarda farklılık elde edilmemiştir. Sarkoptik uyuzun enfekte koyunlarda disülfit dengesini etkilediği düşünülmekte olup bu dengeyi sağlayacak antioksidan moleküllerin terapotik seçeneklere eklenmesi gerektiği öngörülmektedir.
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Corke M, Matthews J: Diagnosing and treating skin diseases in goats: An update. In Practice, 40 (4): 149-156, 2018. DOI: 10.1136/inp.k1595
Nixon EJ, Vineer HR, Wall R: Treatment strategies for sheep scab: An economic model of farmer behaviour. Prev Vet Med, 137, 43-51, 2017. DOI: 10.1016/j.prevetmed.2016.12.015
Woldemeskel M: Dermatophilosis: A threat to livestock production in Ethiopia. Dtsch Tierarztl Wochenschr, 107 (4): 144-146, 2000.
Asmare K, Abebe R, Sheferaw D, Krontveit RI, Barbara W: Mange mite infestation in small ruminants in Ethiopia: Systematic review and meta-analysis. Vet Parasitol, 218, 73-81, 2016. DOI: 10.1016/j. vetpar.2016.01.017
Uzlu E, Karapehlivan M, Erdoğan HM, Kızıltepe Ş, Erkılıç EE, Deveci HA, Gökçe E, Kaya İ, Çitil M: Serum and saliva sialic acid and oxidative stress parameters changes in bulls with foot and mouth disease. Kafkas Univ Vet Fak Derg, 22 (3): 321-325, 2016. DOI: 10.9775/kvfd.2015.13114
Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, SánchezPérez P, Cadenas S, Lamas S: Antioxidant responses and cellular adjustments to oxidative stress. Redox Biol, 6, 183-197, 2015. DOI: 10.1016/ j.redox.2015.07.008
Young IS, Woodside JV: Antioxidants in health and disease. J Clin Pathol, 54 (3): 176-186, 2001. DOI: 10.1136/jcp.54.3.176
Biswas S, Chida AS, Rahman I: Redox modifications of protein-thiols: Emerging roles in cell signaling. Biochem Pharmacol, 71 (5): 551-564, 2006. DOI: 10.1016/j.bcp.2005.10.044
Circu ML, Aw TY: Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med, 48 (6): 749-762, 2010. DOI: 10.1016/j. freeradbiomed.2009.12.022
Matteucci E, Giampietro O: Thiol signalling network with an eye to diabetes. Molecules, 15 (12): 8890-8903, 2010. DOI: 10.3390/ molecules15128890
Tetik S, Ahmad S, Alturfan AA, Fresko I, Disbudak M, Sahin Y, Aksoy H, Yardımcı KT: Determination of oxidant stress in plasma of rheumatoid arthritis and primary osteoarthritis patients. Indian J Biochem Biophys, 47 (6): 353-358, 2010.
Go YM, Jones DP: Cysteine/cystine redox signaling in cardiovascular disease. Free Radic Biol Med, 50 (4): 495-509, 2011. DOI: 10.1016/j. freeradbiomed.2010.11.029
Kuo LM, Kuo CY, Lin CY, Hung MF, Shen JJ, Hwang TL: Intracellular glutathione depletion by oridonin leads to apoptosis in hepatic stellate cells. Molecules, 19 (3): 3327-3344, 2014. DOI: 10.3390/molecules19033327
Prabhu A, Sarcar B, Kahali S, Yuan Z, Johnson JJ, Adam KP, Kensicki E, Chinnaiyan P: Cysteine catabolism: A novel metabolic pathway contributing to glioblastoma growth. Cancer Res, 74 (3): 787- 796, 2014. DOI: 10.1158/0008-5472.CAN-13-1423
Winther JR, Thorpe C: Quantification of thiols and disulfides. Biochim Biophys Acta, 1840 (2): 838-846, 2014. DOI: 10.1016/j.bbagen.2013.03.031
Mourad T, Min KL, Steghens JP: Measurement of oxidized glutathione by enzymatic recycling coupled to bioluminescent detection. Anal Biochem, 283 (2): 146-152, 2000. DOI: 10.1006/abio.2000.4659
Carru C, Deiana L, Sotgia S, Pes GM, Zinellu A: Plasma thiols redox status by laser-induced fluorescence capillary electrophoresis. Electrophoresis, 25 (6): 882-889, 2004. DOI: 10.1002/elps.200305768
Chen W, Zhao Y, Seefeldt T, Guan X: Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid. J Pharm Biomed Anal, 48 (5): 1375-1380, 2008. DOI: 10.1016/j.jpba.2008.08.033
Glowacki R, Bald E: Fully automated method for simultaneous determination of total cysteine, cysteinylglycine, glutathione and homocysteine in plasma by HPLC with UV absorbance detection. J Chromatogr B Analyt Technol Biomed Life Sci, 877 (28): 3400–3404, 2009. DOI: 10.1016/j.jchromb.2009.06.012
Erel O, Neselioglu S: A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem, 47 (18): 326-332, 2014. DOI: 10.1016/j.clinbiochem.2014.09.026
Agegnehu A, Bogale B, Tesfaye S, Dagnachew S: Status of mange infestation in indigenous sheep and goats and their control practices in Wag-Himra zone, Ethiopia. J Vet Med Anim Health, 10 (5): 128-134, 2018. DOI: 10.5897/JVMAH2017.0649
Iqbal A, Baba MA, Shah M, Mushtaq I, Sakina A, Wani S: Treatment of mange infection in a weaner flck of sheep with ivermectin at sheep breeding farm Hardishiva of Kashmir valley. J Parasit Dis, 39 (2): 171-173, 2015. DOI: 10.1007/s12639-013-0305-6
Seid K, Amare S, Tolossa YH: Mange mites of sheep and goats in selected sites of Eastern Amhara region, Ethiopia. J Parasit Dis, 40 (1): 132- 137, 2016. DOI: 10.1007/s12639-014-0463-1
Ural K, Tunca R, Alıç Ural D, Çamkerten İ, Erdoğan H, Ayan A, Gültekin M, Haydardedeoğlu AE, Aysul N, Erdoğan S: Interpretation of serum 25-Hydroxy vitamin D3 concentrations in sheep with naturally occuring sarcoptic mange. J Adv VetBio Sci Tech, 3 (1): 35-40, 2018. DOI: 10.31797/vetbio.413018
Hudson DA, Gannon SA, Thorpe C: Oxidative protein folding: From thiol–disulfide exchange reactions to the redox poise of the endoplasmic reticulum. Free Radic Biol Med, 80, 171-182, 2015. DOI: 10.1016/j. freeradbiomed.2014.07.037
Kundi H, Ates I, Kiziltunc E, Cetin M, Cicekcioglu H, Neselioglu S, Erel O, Ornek E: A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med, 33 (11): 1567- 1571, 2015. DOI: 10.1016/j.ajem.2015.06.016
Camkerten I, Sahin T, Borazan G, Gokcen A, Erel O, Das A: Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Vet Parasitol, 161 (1-2): 106-109, 2009. DOI: 10.1016/j.vetpar.2008.12.019
Aktas MS, Kandemir FM, Kirbas A, Hanedan B, Aydin MA: Evaluation of oxidative stress in sheep infected with Psoroptes ovis using total antioxidant capacity, total oxidant status, and malondialdehyde level. J Vet Res, 61 (2): 197-201, 2017. DOI: 10.1515/jvetres-2017-0025
Dimri U, Bandyopadhyay S, Singh SK, Ranjan R, Mukherjee R, Yatoo MI, Patra PH, De UK, Dar AA: Assay of alterations in oxidative stress markers in pigs naturally infested with Sarcoptes scabiei var. suis. Vet Parasitol, 205 (1-2): 295-299, 2014. DOI: 10.1016/j.vetpar.2014.06.015
Erdoğan H, Camkerten I, Çamkerten G, Ural K, Erdoğan S, Günal İ, Erel Ö: The Effct of hot-iron disbudding on thiol-disulphide homeostasis in calves. Kafkas Univ Vet Fak Derg, 25 (3): 335-339, 2019. DOI: 10.9775/ kvfd.2018.20950