Zhen WANG, Jihai YI, Junbo ZHANG, Huan ZHANG, Chuangfu CHEN, Yuanzhi WANG, Benben WANG

Kuzeybatı Çin’de Atık Koyun Fötuslarından Brucella suis S2 İzolasyonu

Bu çalışma, geleneksel bir koyun çiftliğinde atıkların sebebini araştırmak amacıyla gerçekleştirilmiştir. Çalışmada, 31 hayvan PCR tekniği ile Brucella dış zar protein geni 22 (omp22) için taranmış ve 25’i (%80.6, 25/31) pozitif bulunmuştur. Otuz bir adet atık fötusun 3’ünden Brucella suis S2 ve 10’undan Brucella melitensis izole edilirken bir hayvanda B. suis S2 ve B. melitensis beraber tespit edilmiştir. Tüm bu izolasyonlar hem PCR hem de klasik bakteriyolojik testlerle yapılmıştır. Üç adet izole edilen B. suis ve referans suş B. suis S2 B. suis biovar 1 olarak, 10 adet izole edilen B. melitensis ise B. melitensis biovar 3 olarak identifie edildi. Bu çalışma, B. suis S2’nin gebe koyunlarda atığa sebep olabileceğini göstermiştir. Bu nedenle, gebe koyunlarda koruyucu amaçlı brusellozise karşı yeni bir aşı geliştirmeye gereksinim olduğu sonucuna varılmıştır.

Brucella suis S2 Isolated from Aborted Sheep Fetuses in Northwestern China

This study aimed to investigate the cause of abortion in a traditional sheep farm. Here, 31 specimens were examined by PCR targeting theBrucella outer membrane protein gene 22 (omp22), and 25 (80.6%, 25/31) were found to be positive. Totally, 3 Brucella suis S2 and 10 Brucellamelitensis were isolated from 31 aborted fetuses in which one B. suis S2 and B. melitensis were isolated from a same aborted fetus. All of theseisolates were identified by PCR and conventional bacterial tests. These 3 B. suis isolates and the reference strain B. suis S2 were all identifiedas B. suis biovar 1 and the 10 B. melitensis isolates were all identified as B. melitensis biovar 3. This study suggests that B. suis S2 can partiallyinduce abortion in pregnant ewes. Hence, for the sake of safety, it is need to develop a new Brucella vaccine to protect pregnant sheep frombrucellosis.

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