AHMET KÜRŞAT AZKUR, Serkal GAZYAĞCI, Eren Muhammet ASLAN

Serological and Epidemiological Investigation of Bluetongue, Maedi-Visna and Caprine Arthritis-Encephalitis Viruses in Small Ruminant in Kirikkale District in Turkey

Viral enfeksiyonlar, koyun ve keçilerde yapılan araştırmalar ile önlenebilen önemli sorunlara ve ekonomik kayıplara neden olur. Bu çalışmada mavidil virüsü (BTV), maedi-visna virüs (MVV), caprine arthritis-encephalitis virüs (CAEV)’lerini içeren viral enfeksiyonların, İç Anadolu bölgesinde yer alan Kırıkkale ilinde bulunan koyun ve keçilerde belirlenmesi amaçlanmıştır. Kan örnekleri 279 koyun ve 146 keçiden toplandı ve ELISA, RT-nested PZR ve nested PZR ile test edildi. Serum örneklerinde antikor cevabı BTV (%49.8), CAEV (%7.5) ve MVV (%19.4) seropozitiflik oranı ile tespit edildi. Seropozitif tam kan örneklerinde RT-nested PZR ve nested PZR ile, BTV ve MVV antijenleri sırasıyla (%2.87) (%9.25) oranlarında belirlendi fakat CAEV belirlenemedi. Koyunlarda hem MV hem de BT’ye karşı seropozitif hayvanların oranı %14.6 oranında saptandı. Sonuç olarak Kırıkkale yöresinde koyun ve keçilerde BTV, CAEV ve MVV enfeksiyonlarının doğal varlığının ortaya konulduğu bu çalışma, bu yörede sözü geçen enfeksiyonlarla ilgili yapılan ilk çalışmadır. Bu nedenle enfeksiyonların belirlenmesi için daha detaylı sürvey araştırmalarına ihtiyaç vardır.

Anahtar Kelimeler:

koyun, teşhis, tanı teknikleri, hastalık yaygınlığı, ELISA, hastalık tetkiki, epidemiyolojik araştırmalar, epidemiyoloji, polimeraz zincir reaksiyonu, ters transkriptaz, seroloji, visna maedi virüsü, seroprevalans, viral hastalıklar, mavi dil virüsü, caprine arthritis encephalitis virus, keçi

Kırıkkale Yöresinde Bulunan Küçük Ruminantlarda Mavidil, Maedi-Visna ve Caprine Arthritis-Encephalitis Enfeksiyonlarının Serolojik ve Epidemiyolojik Araştırılması

Viral infections cause important problems and significant economic loses in sheep and goats that can be protected by an investigation of infections. This study aimed to determine the sheep and goat viral infections including bluetongue virus (BTV), maedi-visna virus (MVV) and caprine arthritis-encephalitis virus (CAEV) in Kirikkale city located in Central Anatolia region of Turkey. Blood samples collected from 279 sheep and 146 goats were tested by ELISA, RT-nested PCR and nested PCR. It was detected that antibody response to BTV (49.8%), CAEV (7.5%) and MVV (19.4%) were seropositivity values in all serum samples. BTV (2.87%) and MVV (9.25%) antigens were detected in seropositive whole blood samples via RT-nested PCR and nested PCR but not CAEV. It was found that double positive animals (14.6%) for MVV and BTV in sheep but not goat. As a conclusion sheep and goat were infected with naturally BTV, CAEV and MVV in Kirikkale region. This study showed that this the first report for BTV, MVV and CAEV infections from Kirikkale region. Therefore infections are needed to be further investigations to determine detailed survey studies.

Keywords:

sheep, diagnosis, diagnostic techniques, disease prevalence, ELISA, disease surveys, epidemiological surveys, epidemiology, polymerase chain reaction, reverse transcriptase, serology, visna maedi virus, seroprevalence, viral diseases, bluetongue virus, caprine arthritis encephalitis virus, goats,

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