Sellülaz enziminin buğday samanının besleme değeri, in vitro sindirimi ve mikrobiyal protein üretimi üzerine etkileri

Bu araştırmada, buğday (Triticum aestivum L.) samanı %40 kuru madde (KM) içerecek şekilde, Trichoderma viride kaynaklı sellülazla %0, 0.2, 0.4, 0,6 ve 0.8 dozlarında (KM’de), kontrol ve uygulama başına 3 paralelli olarak işlendikten sonra cam kavanozlara silolanarak oda sıcaklığı (22ºC) ve 40ºC’de 30 gün süre ile inkübasyona bırakılmıştır. Buğday samanının farklı dozlarda sellülaz enzimiyle işlenmesi sonucunda nötr deterjanda çözünmeyen lif (NDF), hemisellüloz ve sellüloz içeriklerinde düşüşler belirlenmiş, suda çözünebilir karbonhidrat (SÇK), laktik asit ve metabolik enerji (ME) içerikleri ise artmıştır (P

Anahtar Kelimeler:

uçucu yağ asitleri, Trichoderma viride, Triticum aestivum, biyokütle üretimi, selülaz, sindirilebilirlik, kuru madde, gaz üretimi, in vitro sindirilebilirlik, besin değeri, organik madde, tek hücre proteini, buğday samanı

Effects of cellulase enzyme on nutritive value, in vitro digestion characteristics and microbial biomass production of wheat straw

Wheat straw (Triticum aestivum L.) was treated by using cellulase from Trichoderma viride at 0, 0.2, 0.4, 0.6, 0.8% doses (dry matter (DM) basis) to have straw containing 40% DM. After this process was performed in triplicate for each control and experimental groups, samples ensiled in glass jars were incubated at room temperature (22ºC) and 40ºC for 30 days. As a result of enzyme treatment, neutral detergent fiber (NDF), hemicellulose and cellulose contents of wheat straws were decreased but water soluble carbohydrate (WSC), lactic acid and metabolizable energy contents (ME) were increased (P<0.05). While significant increases were determined on in vitro gas production (GP), dry matter digestibility (DMD) and true organic matter digestibility (TOMD) parameters of wheat straws as 23.00 ml, 24.30 and 33.40% (in DM), respectively at 40ºC of 0.8% cellulase treatments, microbial biomass production (MBP) was determined as 99.40 mg (in DM) at 40ºC of 0.2% cellulase treatment (P=0.000). In conclusion, when the improvements in the nutrient, cell wall component, and in vitro digestibility were considered, it was determined that the use of fibrolytic enzyme increased the nutritive value of wheat straw. The best results were obtained from higher doses of cellulase (0.8%) treatments at 40ºC.

Keywords:

volatile fatty acids, Trichoderma viride, Triticum aestivum, biomass production, cellulase, digestibility, dry matter, gas production, in vitro digestibility, nutritive value, organic matter, single cell protein, wheat straw,

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