Effects of Nigella sativa aqueous extracts on gastric acid secretion in isolated rat stomach

Çörek otunun farklı şartlarda antioksidant, anti inflamatuar ve anti ülser aktiviteye sahip olduğu gösterilmiştir. Sunulan çalışma izole edilmiş sıçan midesinde çörek otu sulu ekstraktının gastrik asit sekresyonu üzerine etkilerini araştırmak için yapılmıştır. Gastrik asit sekresyonu izole edilmiş sıçan mide preparatında ölçüldü. İn vitro şartlarda yapılan deneylerden elde edilen sonuçlar çörek otu sulu ekstraktının 2 mg/100 ml DMSO ve 5 mg/100 ml DMSO dozlarının histamin ile uyarılan ve 5 mg/100 ml DMSO dozunun bethanechol ile uyarılan gastrik asit sekresyonunu azalttığı, ancak deneyde kullanılan hiçbir dozun pentagastrin ile uyarılan asit sekresyonunu etkilemediğini gösterdi. Sunulan çalışma, çörek otunun gastrik asit sekresyonunu azalttığından dolayı gastroprotektif bir etkiye sahip olduğunu destekliyor.

Anahtar Kelimeler:

gastrik asit, mide, sıçan, pentagastrin, histamin, Nigella sativa, antioksidanlar, betanekol

İzole edilmiş sıçan midesinde çörek otu sulu ekstraktının gastric asit sekresyonu üzerine etkileri

Nigella sativa (NS) has been shown to have antioxidant, anti-inflammatory and antiulcer activities in different conditions. The present work was done to investigate the possible effects of Nigella sativa aqueous extracts (NS) on gastric acid secretion in isolated rat stomach. Gastric acid secretion was measured in the isolated rat stomach preparation. The results obtained in vitro experiments showed that doses of 2 mg/100 ml DMSO and 5 mg/100 ml DMSO of NS aqueous extracts reduced gastric acid secretion stimulated by histamine and 5 mg/100 ml DMSO of NS aqueous extracts reduced gastric acid secretion stimulated by bethanechol, but none of the dosis of NS did affect pentagastrin-induced acid secretion. The present results suggest that NS has a gastroprotective effect, probably due to reducing gastric acid secretion.

Keywords:

gastric acid, stomach, rats, pentagastrin, histamine, Nigella sativa, antioxidants, bethanechol,

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