Roohollah KHEIRI, Reza RANJBAR, Faham KHAMESIPOUR, Leili AKHTARI

Role of Antibiotic in Drug Resistance and Integrons Prevalence in Escherichia coli Isolated from Human and Animal Specimens

Yüksek antibiyotik kullanımı ve dayanıklılık genlerinin yatay transferinin bakterilerde antibiyotik direncinin iki önemli nedeni olduğuna inanılmaktadır. Bu inancı onaylamak veya reddetmek için, insan ve hayvan örneklerinden izole edilen Escherichia coli'nin antimikrobiyel direnci (AMR), Sınıf I ve II integronları ve integron-aracılı direnç prevalansında ilaç uygulamasının rolünü araştırdık. Demografik verileri kaydetmek için; fenotipik antibiyotik duyarlılık testi ve Sınıf I ve II integraz genlerinin tespiti sonrası insan, tavuk, sığır ve koyun gibi farklı örneklerden E. coli izole edildi. İntegronlar ve direnç (p değeri) arasındaki korelasyon SPSS yazılımı kullanılarak değerlendirildi. Demografik kayıtlara göre, tavuklar en yüksek doz ve değişimli antibiyotik aldı. Beklenildiği gibi, en yaygın MDR suşları ve integronları tavuk suşlarında bulundu. Ki kare analizi, başka örnekler yerine çoğunlukla tavuktan izole edilen E. coli suşlarının integronları ve direnç modeli arasında anlamlı bir korelasyon gösterdi. Araştırmamız, antibiyotik kullanımının ortakçı E. coli'de antibiyotik direnci ve integronların yaygınlığı ile sıkı ilişkili olduğunu doğruladı. Bu sonuçlar, yüksek doz antibiyotik ve seçim baskısının dirençli olanların ardından duyarlı bağırsak mikroorganizmalarını ortadan kaldırabildiğini doğrulamaktadır.

İnsan ve Hayvan Örneklerinden İzole Edilen Escherichia coli'nin İlaç Direnci ve İntegronların Yaygınlığında Antibiyotik Rolü

It is believed that high antibiotic consumption and horizontal transfer of resistance genes are two major causes of antibiotic resistance in bacteria. To confirm or reject this belief, we studied the role of drug administration in antimicrobial resistance (AMR), prevalence of Class I and II integrons and integron-mediated resistance in Escherichia coli isolated from human and animal specimens. Recording demographic data, E. coli from different specimens including human, chicken, cattle and sheep was isolated followed by phenotypic antibiotic susceptibility testing and detection of Class I and II integrase genes. The correlation between integrons and resistance (P value) was evaluated using SPSS software. According to demographic records, chickens received the highest dose and variation of antibiotics. As expected, the most prevalent MDR strains and integrons were found in chicken strains. Chi square analysis showed a significant correlation between integrons and resistance pattern mostly in E. coli strains isolated from chicken rather than other specimens. Our survey confirmed that the use of antibiotics is strongly associated with the prevalence of antimicrobial resistance and integrons in commensal E. coli. Such results confirm that high doses of antibiotics and selection pressure may remove susceptible intestinal microorganisms followed by resistant ones.

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