MUSTAFA SİNAN AKTAŞ, FATİH MEHMET KANDEMİR, MUSTAFA ÖZKARACA, BAŞAK HANEDAN, AKIN KIRBAŞ

Protective Effects of Rutin on Acute Lung Injury Induced by Oleic Acid in Rats

Bu çalışmanın amacı ratlarda rutinin farklı dozlarının antioksidan ve anti-inflamatuar özellikleri yoluyla oleik asit (OA) ile oluşturulan akut akciğer hasarı (ALI) üzerine koruyucu etkilerini araştırmaktı. 35 adet Sprague-Dawley erkek rat kontrol, rutin 150 mg, OA, rutin 75 mg + OA ve rutin 150 mg + OA olmak üzere rasgele 5 gruba ayrıldı. Rutin 75 mg + OA ve rutin 150 mg + OA gruplarında akciğer malondialdehit (MDA) düzeyi OA grubununkine göre önemli düzeyde düşüktü. Rutin 75 mg + OA ve rutin 150 mg + OA gruplarında akciğer GPx (glutasyon peroksidaz), CAT (katalaz), SOD (süperoksit dismutaz) aktiviteleri ile GSH (glutatyon) düzeyleri OA grubununkilere göre önemli düzeyde yüksekti ve kontrol grubununkilere göre önemli düzeyde düşüktü. Rutin 75 mg + OA ve rutin 150 mg + OA gruplarında akciğerlerin interstisyel kısımlarında iNOS ekspresyonları OA grubunkilere göre önemli düzeyde düşüktü. OA ile oluşturulan ALI'de rutinin antioksidan ve antiinflamatuvar özellikler aracılığıyla koruyucu etkilere sahip olduğu, ALI'de destekleyici tedavi amacıyla rutin uygulamasının pratik olarak yararlı olduğu tespit edildi

Ratlarda Oleik Asit Kaynaklı Akut Akciğer Hasarı Üzerine Rutinin Koruyucu Etkileri

The purpose of this study was to explore the protective effects of different doses of rutin with the antioxidant and anti-inflammatory properties on acute lung injury (ALI) induced by oleic acid (OA) in rats. Thirty-five Sprague-Dawley male rats were randomly separated into five groups comprising control, rutin 150 mg, OA, rutin 75 mg + OA and rutin 150 mg + OA. In the rutin 75 mg + OA and rutin 150 mg + OA groups, the lung malondialdehyde level (MDA) was significantly lower than that of the OA group. In the rutin 75 mg + OA and and rutin 150 mg + OA groups, the lung GPx (glutathione peroxidase), CAT (catalase) and SOD (superoxide dismutase) activities and GSH (glutathione) levels were significantly higher than those of the OA group, and significantly lower than those of the control group. iNOS expressions in the interstitial parts of the lungs were significantly lower than those of the OA group. The iNOS expression was lower in the 150 mg + OA group compared to the rutin 75 mg + OA group. It was concluded that on the ALI induced by OA, rutin had protective effects through the antioxidant and antiinflammatory properties and that the treatment of rutin as a supportive treatment in ALI was found to be practically useful

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