Murat Sırrı AKOSMAN, Hasan Hüseyin DEMİREL, RUHİ TÜRKMEN

Protective Effect of Resveratrol on Kidney and Liver Histopathology Induced by NMDA Receptor Antagonist Mk-801 in Mice

TMK-801 is an antagonist of N-methyl-D-aspartate (NMDA) receptors. Sub-chronic administration of 1 mg / kg dose of MK-801 resulted in schizophrenia-like symptoms and degeneration in the brain of mice. In this study, it was investigated whether this dose could cause histopathological changes in kidney and liver tissues and resveratrol had a protective role against these changes. For this aim, 24 male mice were obtained and divided equally into 4 groups. The animals in the control group were intraperitoneally (i.p.) given 10 mL/kg saline solution, the animals in the experiment groups were i.p. given 1 mg/kg MK-801 alone, 40 mg/kg resveratrol alone and MK-801 by 1 mg/kg for 14 days. The kidney and the liver of the sacrificed mice were collected and routine histology procedure was applied. Glomerular shrinkage was observed in the kidneys after the measurements. Besides this finding, histopathological changes were observed in both kidney and liver tissue and resveratrol inhibited most of the harmful effcts. In conclusion, MK-801 caused histopathological changes in both kidney and liver tissues, and resveratrol had a significantly protective role on this injury.

Farelerde NMDA Reseptör Antagonisti Mk-801 İle Böbrek ve Karaciğerde Oluşan Histopatolojik Değişikliklere Karşı Resveratrolün Koruyucu Etkisi

MK-801 N-methyl-D-aspartate (NMDA) reseptör antagonisti bir kimyasal ajandır. Bu kimyasalın sub-kronik 1 mg/kg dozunda uygulanması farelerde şizofreni benzeri semptomlar görülmesine ve beyinde dejenerasyona yol açmaktadır. Resveratrolünse hem böbrek hem de karaciğer üzerinde koruyucu etkisi vardır. Sunulan bu çalışmada hem bu dozun karaciğer ve böbrek gibi organlara histopatalojik bir zararının olup olmadığı hem de resveratrolün koruyucu etkisinin olup olmadığı araştırılmıştır. Bu amaçla 24 erkek fare alınmış ve 4 eşit gruba bölünmüştür. Kontrol grubuna intraperitoneal (i.p.) olarak %0.9’luk (10 mL/kg) fizyolojik tuzlu su verilmiştir. Deney gruplarındaki hayvanlara 14 gün boyunca MK-801 tek başına 1 mg / kg, resveratrol tek başına 40 mg/kg ve MK-801 ile birlikte i.p. verildi. Farelerin sakrifikasyonundan sonra karaciğer ve böbrek dokuları çıkartılmış, histolojik işlemlerden sonra hematoksilen-eozinle boyanmıştır. Ölçümler sonucunda glomeruluslarda istatistiksel olarak küçülme ile birlikte karaciğer ve böbrek dokusunda histopatolojik değişikliklere rastlanmıştır. Resveratrolün bu zararlı değişikliklere karşı oldukça fazla koruyucu etkisinin olduğu gözlenmiştir. Sonuç olarak MK-801’in kronik dozunun karaciğer ve böbrek üzerine zararlı etkileri olduğu ve resveratrolün bu zararlı etkilere karşı önemli miktarda bir koruyucu özelliğinin olduğu gözlemlenmiştir.

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