Ali Reha AĞAOĞLU, Özgecan KORKMAZ AĞAOĞLU, Selim ASLAN, Mesih KOCAMÜFTÜOĞLU, Afşin KÖKER, Yunus ÇETİN, Örsan GÜNGÖR, Dilek OZTURK, Mustafa SAATCI

The Effects of Presynch-10 and Ovsynch on Some Endometrial Tolland Nod-like Receptor Gene Expressions in Repeat Breeder Cows

Subclinical endometritis (SE) is one cause of repeat breeder syndrome (RBS). When endometrial cells come into contact with antigens during inflmmatory events like endometritis, they act like innate immune system cells. Ovsynch-based therapies stimulate the innate immune system, which is how they may be able to cure SE and then RBS. This study aimed to assess the effcts of presynch-10 and ovsynch applications on mRNA expressions of some endometrial Toll- and Nod-like receptors (TLRs and NLRs) in cows with RBS. A total of 40 repeat breeder cows were used in the study. The presynch-10 and ovsynch protocols were used in group 1 (G1, n=20), and no protocol was used in group 2 (G2, n=20). Endometrial samples were collected before and after the synchronization protocols in G1. In G2, samples were collected, and after waiting time up to the duration of synchronization protocol in G1, samples were taken again. The expression profiles of the genes being investigated were examined using RT-qPCR. NLRC4, NALP3 and TLR4 were basically expressed in all of the samples while NOD2 expression was completely undetectable. There was a statistical diffrence when NOD1 and TLR2 expressions in the second samples were compared between G1 and G2 (P

Repeat Breeder İneklerde Presynch-10 ve Ovsynch Uygulamalarının Bazı Endometriyal Toll ve Nod Benzeri Reseptör Genlerinin Ekspresyonları Üzerine Etkileri

Subklinik endometritis (SE), repeat breeder sendromunun (RBS) nedenlerinden biridir. Endometriyal hücreler, endometritis gibi yangısal olaylar sırasında antijenlerle karşılaştıklarında, doğal bağışıklık sistemi hücreleri gibi davranırlar. Ovsynch temelli tedaviler doğal bağışıklık sistemini uyararak SE ve sonra RBS’yi tedavi edebilmektedirler. Bu çalışma presynch-10 ve ovsynch uygulamalarının RBS’li ineklerde bazı endometrial Toll- ve Nod- benzeri reseptörlerin (TLR’ler ve NLR’ler) mRNA ekspresyonları üzerindeki etkilerini değerlendirmeyi amaçlamıştır. Çalışmada toplam 40 repeat breeder inek kullanılmıştır. Grup 1’de (G1, n=20), presynch-10 ve ovsynch uygulanmış, Grup 2’de herhangi bir uygulama yapılmamıştır (G2, n=20). G1’de senkronizasyon uygulamaları öncesi ve sonrasında endometriyal örnekler alınmıştır. G2’de örnekler alındıktan sonra G1’de uygulanan senkronizasyon protokolü süresi kadar beklenerek örneklemeler tekrarlanmıştır. İncelenen genlerin ekpresyon düzeyleri RT-qPCR ile belirlenmiştir. NLRC4, NALP3 ve TLR4’ün temel olarak tüm örneklerde eksprese edildiği, NOD2 ekspresyonunun ise belirlenemeyecek seviyelerde kaldığı saptanmıştır. G1 ve G2’den alınan ikinci endometriyal örnekler arasında NOD1 ve TLR2 ekspresyonları arasında istatistiksel bir fark oluşmuştur (P

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