The objectives of this cross-sectional study were to detect the presence of small ruminant lentiviral infections in Kosovo and estimate the serological prevalence for the year of 2016 . A total of 5.272 sheep and 435 goats were tested using a commercially available indirect enzymelinked immunosorbent assay (ELISA) for Maedi-Visna/Caprine Arthritis-Encephalitis, giving an overall individual sero-prevalence in sheep of 34.8% (95% confidence interval 31.8% to 38.0%), and a flck prevalence of 85%, and in goats an overall individual sero-prevalence of 15.6% (95% confidence interval 7.2% to 25.6%) and flck prevalence of 35%. Sero-prevalence in sheep was higher in the South and West of Kosovo, whereas in goats was higher in the East and South. There were no statistically significant diffrences in sero-prevalence between sheep in diffrent age groups
Bu kesitsel çalışmanın amacı, Kosova’da küçük ruminant lentiviral enfeksiyonların varlığını saptamak ve 2016 yılı için serolojik prevalansı tahmin etmekti. Toplam 5.272 koyun ve 435 keçi ticari olarak temin edilebilen dolaylı enzim bağlı immünosorbent test (ELISA) kullanılarak Maedi-Visna/Keçi Artritis-Ensefalitis hastalığı yönünden kontrol edildi. Koyunlarda bireysel sero-prevalans %34.8 (%31.8 ila %38.0; %95 güven aralığı), sürü prevalansı % 85 bulunurken keçilerde %15.6’lık bireysel sero- prevalans (%7.2 ila %25.6; %95 güven aralığı) ve %35’lik sürü prevalansı belirlendi. Hastalık sero-prevalansı koyunlarda Kosova’nın güneyinde ve batısında, keçilerde ise doğusu ve güneyinde daha yüksekti. Koyunlarda
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1. Ramírez H, Reina R, Amorena B, de Andrés D, Martínez HA: Small ruminant lentiviruses: Genetic variability, tropism and diagnosis. Viruses, 5 (4): 1175-1207, 2013. DOI: 10.3390/v5041175
2. Minguijón E, Reina R, Pérez M, Polledo L, Villoria M, Ramírez H, Leginagoikoa I, Badiola JJ, García-Marín JF, de Andrés D, Luján L, Amorena B, Juste RA: Small ruminant lentivirus infections and diseases. Vet Microbiol, 181 (1-2): 75-89, 2015. DOI: 10.1016/j.vetmic.2015.08.007
3. Minardi da Cruz JC, Singh DK, Lamara A, Chebloune Y: Small ruminant lentiviruses (SRLVs) break the species barrier to acquire new host range. Viruses, 5 (7): 1867-1884, 2013. DOI: 10.3390/v5071867
4. Shah C, Huder JB, Böni J, Schönmann M, Mühlherr J, Lutz H, Schüpbach J: Direct evidence for natural transmission of small-ruminant lentiviruses of subtype A4 from goats to sheep and vice versa. J Virol, 78 (14): 7518-7522, 2004. DOI: 10.1128/JVI.78.14.7518-7522.2004
5. Peterhans E, Greenland T, Badiola J, Harkiss G, Bertoni G, Amorena B, Eliaszewicz M, Juste RA, Krassnig R, Lafont JP, Lenihan P, Pétursson G, Pritchard G, Thorley J, Vitu C, Mornex JF, Pépin M: Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes. Vet Res, 35 (3): 257-274, 2004. DOI: 10.1051/vetres:2004014
6. Blacklaws BA, Berriatua E, Torsteinsdottir S, Watt NJ, de Andres D, Klein D, Harkiss GD: Transmission of small ruminant lentiviruses. Vet Microbiol, 101 (3): 199-208, 2004. DOI: 10.1016/j.vetmic.2004.04.006
7. Heaton MP, Clawson ML, Chitko-Mckown CG, Leymaster KA, Smith TP, Harhay GP, White SN, Herrmann-Hoesing LM, Mousel MR, Lewis GS, Kalbflisch TS, Keen JE, Laegreid WW: Reduced lentivirus susceptibility in sheep with TMEM154 mutations. PLoS Genet, 8 (1): e1002467, 2012. DOI: 10.1371/journal.pgen.1002467
8. Pépin M, Vitu C, Russo P, Mornex JF, Peterhans E: Maedi-visna virus infection in sheep: A review. Vet Res, 29 (3-4): 341-367, 1998.
9. Sigurdsson B, Grimsson H, Palsson PA: Maedi, a chronic, progressive infection of sheep’s lungs. J Infect Dis, 90 (3): 233-241, 1952. DOI: 10.1093/ infdis/90.3.233
10. Sigurdsson B, Palsson PA: Visna of sheep. A slow demyelinating infection. Br J Exp Pathol, 39 (5): 519-528, 1958.
11. Benavides J, García-Pariente C, Ferreras M, Fuertes M, GarcíaMarín F, Pérez V: Diagnosis of clinical cases of the nervous form of Maedi-Visna in 4- and 6-month-old lambs. Vet J, 174 (3): 655-658, 2007. DOI: 10.1016/j.tvjl.2006.10.014
12. Akkoc A, Kocaturk M, Alasonyalilar Demirer A, Şenturk S, Renzoni G, Preziuso S: Maedi-Visna virus infection in a Merino lamb with nervous signs. Turk J Vet Anim Sci, 35, 467-470, 2011. DOI: 10.3906/vet-1102-784
13. Hamza LA, Özkan C: Serological investigation of Maedi-Visna in sheep with chronic respiratory disease in Erbil, Iraq. Atatürk Üniv Vet Bil Derg, 12 (3): 227-234, 2017. DOI: 10.17094/ataunivbd.295120
14. Crawford TB, Adams DS, Cheevers WP, Cork LC: Chronic arthritis in goats caused by a retrovirus. Science, 207 (4434): 997-999, 1980. DOI: 10.1126/science.6153243
15. Cork LC, Hadlow WJ, Crawford TB, Gorham JR, Piper RC: Infectious leukoencephalomyelitis of young goats. J Infect Dis, 129 (2): 134-141, 1974. DOI: 10.1093/infdis/129.2.134
16. van der Molen EJ, Vecht U, Houwers DJ: A chronic indurative mastitis in sheep, associated with maedi/visna virus infection. Vet Q, 7 (2): 112-119, 1985. DOI: 10.1080/01652176.1985.9693966
17. Gregory L, Birgel Junior E, Lara M, Angelini M, Araújo W, Rizzo H, Maiorka P, Castro R, Kiraly A, Benesi F, Birgel E: Clinical features of indurative mastitis caused by caprine arthritis encephalitis virus. Braz J Vet Pathol, 2 (2): 64-68, 2009.
18. Herrmann-Hoesing LM: Diagnostic assays used to control small ruminant lentiviruses. J Vet Diagn Invest, 22 (6): 843-855, 2010. DOI: 10.1177/104063871002200602
19. de Andrés X, Ramírez H, Bertolotti L, San Román B, Glaria I, Crespo H, Jáuregui P, Minguijón E, Juste R, Leginagoikoa I, Pérez M, Luján L, Badiola JJ, Polledo L, García-Marín JF, Riezu JI, BorrásCuesta F, de Andrés D, Rosati S, Reina R, Amorena B: An insight into a combination of ELISA strategies to diagnose small ruminant lentivirus infections. Vet Immunol Immunopathol, 152 (3-4): 277-288, 2013. DOI: 10.1016/j.vetimm.2012.12.017
20. Czopowicz M, Szaluś-Jordanob O, Mickiewicz M, Witkowski L, Moroz A, Markowska-Daniel I, Reczyńska D, Bagnicka E, Kaba J: Fall in antibody titer to small ruminant lentivirus in the periparturient period in goats. Small Ruminant Res, 147, 37-40, 2017. DOI: 10.1016/j. smallrumres.2016.12.006
21. Muz D, Oğuzoğlu TC, Rosati S, Reina R, Bertolotti L, Burgu I: First molecular characterization of visna/maedi viruses from naturally infected sheep in Turkey. Arch Virol, 158 (3): 559-570, 2013. DOI: 10.1007/s00705- 012-1518-1
22. Mitrov D, Naletoski I, Dzadzovski I, Krstevski K, Ulcar I, Celeska I: Serological diagnostic of maedi-visna (MVV) in sheep and Caprine arthritis encephalitis virus (CAEV) in goats in Macedonia. Macedonian Vet Rev, 32 (2): 5-10, 2009.
23. Vidić B, Grgić Ž, Savić-Jevđenić S, Maljković M, Milanov D: Ispitivanje raširenosti maedi-visna u zapatima ovaca. Arhiv Vet Med, 1 (1): 30-40, 2008.
24. Angelopoulou K, Karanikolaou K, Papanastasopoulou M, KoumpatiArtopiou M, Vlemmas I, Papadopoulos O, Koptopoulos G: First partial characterisation of small ruminant lentiviruses from Greece. Vet Microbiol, 109 (1-2): 1-9, 2005. DOI: 10.1016/j.vetmic.2005.04.025
25. Masalski N, Dikova T, Bonev S, Chernev T: Serologic diagnosis of Maedi-Visna in sheep. Veterinarska Medicina, 5 (3-4): 165-168, 1999.
26. Rodica-Gurau M, Baraitareanu S, Danes D: Serological survey sf Caprine Arthritis-Encephalitis Virus infection in a southeastern Romanian farm. Scientific Works. Series C. Veterinary Medicine, LXI (2): 169-171, 2015.
27. Azkur AK, Gazyagcis S, Aslan ME: Serological and epidemiological investigation of bluetongue, maedi-visna and caprine arthritis-encephalitis viruses in small ruminant in Kirikkale District in Turkey. Kafkas Univ Vet Fak Derg, 17 (5): 803-808, 2011. DOI: 10.9775/kvfd.2011.4492
28. Albayrak H, Yazici Z, Okur-Gumusova S, Ozan E: Maedi-visna virus infection in Karayala and Amasya Herik breed sheep from provinces in northern Turkey. Trop Anim Health Prod, 44 (5): 939-941, 2012. DOI: 10.1007/s11250-011-9996-9
29. Herrmann-Hoesing LM, Palmer GH, Knowles DP: Evidence of proviral clearance following postpartum transmission of an ovine lentivirus. Virology, 362 (1): 226-234, 2007. DOI: 10.1016/j.virol.2006.12.021
30. OpenEpi: http://openepi.com/Menu/OE_Menu.htm; Accessed: 24.08.2017
31. Wilson EB: Probable inference, the law of succession, and statistical inference. J Am Stat Assoc, 22, 209-212, 1927. DOI: 10.1080/ 01621459. 1927.10502953
32. Rothman KJ, Boice JD: Epidemiologic analysis with a programmable calculator. NIH Pub No. 79-1649., 31-32, Bethesda, Md.: U.S. Dept. of Health, Education, and Welfare, Public Health Service, National Institutes of Health, 1979.
33. Wallis SA: Binomial confidence intervals and contingency tests: Mathematical fundamentals and the evaluation of alternative methods. J Quant Linguist, 20 (3): 178-208, 2013. DOI: 10.1080/09296174. 2013.799918
34. Bennett S, Woods T, Liyanage WM, Smith DL: A simplified general method for cluster-sample surveys of health in developing countries. World Health Stat Q, 44 (3): 98-106, 1991.
35. Kamberi M: Country Pasture/Forage Resource Profiles -Kosovo. Food and Agriculture Organization of the United Nations (FAO), 2009.
36. Brinkhof J, van Maanen C: Evaluation of five enzyme-linked immunosorbent assays and an agar gel immunodiffusion test for detection of antibodies to small ruminant lentiviruses. Clin Vaccine Immunol, 14 (9): 1210-1214, 2007. DOI: 10.1128/CVI.00282-06
37. Beato MS, Bertoni G, Nardelli S, Stifter E, Tavella A: The complex serological monitoring of an ongoing Small Ruminant Lentiviruses eradication campaign. In, Parallel EPIZONE Poster Presentation, Montpellier-France, 2015.
38. Lacerenza D, Giammarioli M, Grego E, Marini C, Profiti M, Rutili D, Rosati S:Antibody response in sheep experimentally infected with diffrent small ruminant lentivirus genotypes. Vet Immunol Immunopathol, 112 (3- 4): 264-271, 2006. DOI: 10.1016/j.vetimm.2006.03.016
39. Reina R, Grego E, Profiti M, Glaria I, Robino P, Quasso A, Amorena B, Rosati S: Development of specific diagnostic test for small ruminant lentivirus genotype E. Vet Microbiol, 138 (3-4): 251-257, 2009. DOI: 1 0.1016/j.vetmic.2009.04.005