Yue LIU, Ling YANG, Xiaolei YAO, Shengqin ZANG, Xiaoyong CHEN, Hongxin SUN, Weitao DUN

Koyunlarda Östrus İndüksiyonu Sonrası Ovaryum, Oviduct ve Endometriyumda Folikül Uyarıcı Hormon, Luteotropik Hormon, Östrojen ve Progesteron Reseptör Ekspresyonu

Östrusun indüksiyonu, koyunlarda gebelik oranını artırmak için etkili bir yönetim aracıdır ve kısa kuyruklu Han koyununda ilkbaharda kuzulamanın ardından uzun bir östrus aralığı söz konusudur. Bu çalışmada, kısa kuyruklu Han koyunlarında östrus uyarımı için intravaginal salınan norgestrel (PRID) ve PRID + gebe kısrak serum gonadotropini (PMSG) kullanıldı. Ovidukt ampullası, ovaryum stroması ve endometriyumdaki folikül uyarıcı hormon reseptörü (FSHR), luteinleştirici hormon reseptörü (LHR), östrojen reseptörü (ER) ve progesteron reseptörü (PGR) mRNA ekspresyonu düzeyleri kantitatif real-time PCR ile analiz edildi. Sonuçlar, koyunlarda PRID ve PRID+PMSG ile östrus indüksiyonunun, östrustaki hayvan yüzdesi ve konsepsiyon oranını arttırmada neredeyse aynı etkilere sahip olduğunu ve östrus indüksiyonunun ovidukt ampullası, ovaryum stroması ve endometriyumdaki LHR, ER ve PGR mRNA ekspresyonu ile ovaryum stroması ve endometriyumdaki FSHR üzerinde anlamlı bir etkisi olmadığını gösterdi. Bunun yanısıra, östrus indüksiyonunun, koyunlarda ovidukt ampullasında FSHR mRNA’nın ekspresyonunu önemli ölçüde azaltabileceği belirlendi (P

Expression of Follicle-stimulating Hormone, Luteotropic Hormone, Estrogen and Progesterone Receptors in Ovary, Oviduct and Endometrium After Estrus Induction in Ewe

Induction of estrus is an effctive management tool for increasing the pregnancy rate in ewes, and there is a long estrus interval afterlambing in spring in Small-tail Han ewe. In this study, norgestrel releasing intra-vaginal devices (PRID) and PRID + pregnant mare serumgonadotrophin (PMSG) were used for estrus induction in Small-tail Han ewe, and expression of follicle-stimulating hormone receptor (FSHR),luteinizing hormone receptor (LHR), estrogen receptor (ER) and progesterone receptor (PGR) mRNA in the ovarial stroma, oviduct ampullaand endometrium was analyzed by quantitative real-time PCR. The results showed that the estrus-induction with PRID and PRID + PMSGalmost had the same effcts on increasing the percentage of animals in estrus and conception rate of the ewes, and the estrus-induction hadno significant effct on the expression of LHR, ER and PGR mRNA in the oviduct ampulla, ovarial stroma and endometrium, and FSHR in theovarial stroma and endometrium. Furthermore, the estrus-induction could significantly decrease the expression of FSHR mRNA in the ovineoviduct ampulla (P

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1. Ungerfeld R, Rubianes E: Short term primings with different progestogen intravaginal devices (MAP, FGA and CIDR) for eCG-estrus induction in anestrus ewes. Small Ruminant Res, 46, 63-66, 2002. DOI: 10.1016/s0921-4488(02)00105-0
2. Husein MQ, Kridli RT: Effct of progesterone prior to GnRH-PGF2α treatment on induction of oestrus and pregnancy in anestrus Awassi ewes. Reprod Domest Anim, 38, 228-232, 2003. DOI: 10.1046/j.1439- 0531.2003.00411.x
3. Martinez MF, McLeod B, Tattersfield G, Smaill B, Quirke LD, Juengel JL: Successful induction of oestrus, ovulation and pregnancy in adult ewes and ewe lambs out of the breeding season using a GnRH+progesterone oestrus synchronisation protocol. Anim Reprod Sci, 155, 28-35, 2015. DOI: 10.1016/j.anireprosci.2015.01.010
4. Antunes-Melo KD, Oliveira VSD, Santos ADF, Oliveira CAD, Mendonça LM, Goveia JSS, Almeida TS: Use of reduced doses of ECG applied by diffrent routes in the TAI program in Santa Ines sheep. Semina Ciencias Agrarias, 36, 1347-1354, 2015. DOI: 10.5433/1679-0359.2015v36 n3p1347
5. Zonturlu AK, Özyurtlu, N, Kaçar C: Effct of diffrent doses PMSG on estrus synchronization and fertility in Awassi ewes synchronized with progesterone during the transition period. Kafkas Univ Vet Fak Derg, 17, 125-129, 2011. DOI: 10.9775/kvfd.2010.2572
6. Miranda VO, Oliveira FC, Dias JH, Vargas Júnior SF, Goularte KL, Sá Filho MF, Sá Filho OG, Baldassarre H, Vieira AD, Lucia T Jr, Gasperin BG: Estrus resynchronization in ewes with unknown pregnancy status. Theriogenology, 106, 103-107, 2018. DOI: 10.1016/j. theriogenology.2017.10.019
7. Wei S, Chen S, Wei B, Liu Z, Bai T, Lin J: Estrus synchronization schemes and application efficacies in anestrus lanzhou fat-tailed ewes. J Appl Anim Res, 44, 466-473, 2016. DOI: 10.1080/09712119.2015.1091350
8. Dogan I, Konyali A, Gunay U, Yurdabak S: Comparison of the effct of cronolone sponges and PMSG or cloprostenol on estrus induction in Turkish Saanen goats. Pol J Vet Sci, 11, 29-34, 2008.
9. Eugster EA: The use of gonadotropin-releasing hormone analogs beyond precocious puberty. J Pediatr, 167, 481-485, 2015. DOI: 10.1016/j. jpeds.2015.05.031
10. Ohlsson B: Gonadotropin-releasing hormone and its physiological and pathophysiological roles in relation to the structure and function of the gastrointestinal tract. Eur Surg Res, 57, 22-33, 2016. DOI: 10.1159/ 000445717
11. Das N, Kumar TR: Molecular regulation of follicle-stimulating hormone synthesis, secretion and action. J Mol Endocrinol, 60, R131-R155, 2018. DOI: 10.1530/JME-17-0308
12. McDonald R, Sadler C, Kumar TR: Gain-of-function genetic models to study FSH action. Front Endocrinol (Lausanne), 10, 28, 2019. DOI: 10.3389/fendo.2019.00028
13. Blair JA, Bhatta S, McGee H, Casadesus G: Luteinizing hormone: Evidence for direct action in the CNS. Horm Behav, 76, 57-62, 2015. DOI: 10.1016/j.yhbeh.2015.06.020
14. Woelders H, van der Lende T, Kommadath A, te Pas MF, Smits MA, Kaal LM: Central genomic regulation of the expression of estrus behaviour in dairy cows: A review. Animal, 8, 754-764, 2014. DOI: 10.1017/ S1751731114000342
15. Bairagi S, Grazul-Bilska AT, Borowicz PP, Reyaz A, Valkov V, Reynolds LP: Placental development during early pregnancy in sheep: Progesterone and estrogen receptor protein expression. Theriogenology, 114, 273-284, 2018. DOI: 10.1016/j.theriogenology.2018.04.002
16. Grazul-Bilska AT, Thammasiri J, Kraisoon A, Reyaz A, Bass CS, Kaminski SL, Navanukraw C, Redmer DA: Expression of progesterone receptor protein in the ovine uterus during the estrus cycle: Effects of nutrition, arginine and FSH. Theriogenology, 108, 7-15, 2018. DOI: 10.1016/j.theriogenology.2017.11.008
17. Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25, 402-408, 2001. DOI: 10.1006/meth.2001.1262
18. Maxwell WMC, Barnes DR: Induction of oestrus in ewes using a controlled internal drug release device and PMSG. J Agric Sci, 106, 201- 203, 1986. DOI: 10.1017/S0021859600061931
19. Ozyurtlu N, Kucukaslan I, Cetin Y: Characterization of estrus induction response, estrus duration, fecundity and fertility in Awassi ewes during the non-breeding season utilizing both CIDR and intravaginal sponge treatments. Reprod Domest Anim, 45, 464-467, 2010. DOI: 10.1111/ j.1439-0531.2008.01246.x
20. Jayaraman A, Kumar TR: Extra-pituitary expressed follicle-stimulating hormone: Is it physiologically important? Biol Reprod, 97, 622-626, 2017. DOI: 10.1093/biolre/iox117
21. Ulloa-Aguirre A, Zariñán T, Jardón-Valadez E, Gutiérrez-Sagal R, Dias JA: Structure-function relationships of the follicle-stimulating hormone receptor. Front Endocrinol (Lausanne), 9, 707, 2018. DOI: 10.3389/fendo.2018.00707
22. Fan QR, Hendrickson WA: Structure of human follicle-stimulating hormone in complex with its receptor. Nature, 433, 269-277, 2005. DOI: 10.1038/nature03206
23.Cárdenas H, Pope WF: Androgen receptor and follicle-stimulating hormone receptor in the pig ovary during the follicular phase of the estrus cycle. Mol Reprod Dev, 62, 92-98, 2002. DOI: 10.1002/mrd.10060
24. Grazul-Bilska AT, Reyaz A, Valkov V, Dorsam ST, Redmer DA: Follicle stimulating hormone receptor protein is expressed in ovine uterus during the estrus cycle and utero-placenta during early pregnancy: An immunohistochemical study. Acta Histochem, 120, 420-428, 2018. DOI: 10.1016/j.acthis.2018.05.005
25. Małysz-Cymborska I, Andronowska A: Downregulation of LH and FSH receptors after hCG and eCG treatments in the porcine oviduct. Domest Anim Endocrinol, 57, 48-54, 2016. DOI: 10.1016/j.domaniend.2016.06.003
26. Dufau ML: The luteinizing hormone receptor. Annu Rev Physiol, 60, 461-496, 1998. DOI: 10.1146/annurev.physiol.60.1.461
27. Narayan P: Genetic models for the study of luteinizing hormone receptor function. Front Endocrinol (Lausanne), 6:152, 2015. DOI: 10.3389/ fendo.2015.00152
28. Gawronska B, Stepien A, Ziecik AJ: Effct of estradiol and progesterone on oviductal LH-receptors and LH-dependent relaxation of the porcine oviduct. Theriogenology, 53, 659-672, 2000. DOI: 10.1016/S0093-691X (99)00265-4
29. Kasahara Y, Kitahara Y, Nakamura K, Minegishi T: Downregulation of LH receptor mRNA in the rat uterus. Mol Med Rep, 5, 1146-1150, 2012. DOI: 10.3892/mmr.2012.795
30. Wang LH, Zhang W, Gao QX, Wang F: Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrus cycle. Genet Mol Res, 11, 3766-3780, 2012. DOI: 10.4238/2012.October.15.8
31. Liu J, Aronow BJ, Witte DP, Pope WF, La Barbera AR: Cyclic and maturation-dependent regulation of follicle-stimulating hormone receptor and luteinizing hormone receptor messenger ribonucleic acid expression in the porcine ovary. Biol Reprod, 58, 648-658, 1998. DOI: 10.1095/ biolreprod58.3.648
32. Lin CY, Ström A, Vega VB, Kong SL, Yeo AL, Thomsen JS, Chan WC, Doray B, Bangarusamy DK, Ramasamy A, Vergara LA, Tang S, Chong A, Bajic VB, Miller LD, Gustafsson JA, Liu ET: Discovery of estrogen receptor alpha target genes and response elements in breast tumor cells. Genome Biol, 5, R66, 2004. DOI: 10.1186/gb-2004-5-9-r66
33. Cárdenas H, Burke KA, Bigsby RM, Pope WF, Nephew KP: Estrogen receptor beta in the sheep ovary during the estrus cycle and early pregnancy. Biol Reprod, 65, 128-134, 2001. DOI: 10.1095/ biolreprod65.1.128
34. Berisha B, Pfaffl MW, Schams D: Expression of estrogen and progesterone receptors in the bovine ovary during estrus cycle and pregnancy. Endocrine, 17, 207-214, 2002. DOI: 10.1385/endo:17:3:207
35. Spencer TE, Bazer FW: Temporal and spatial alterations in uterine estrogen receptor and progesterone receptor gene expression during the estrus cycle and early pregnancy in the ewe. Biol Reprod, 53, 1527- 1543, 1995. DOI: 10.1095/biolreprod53.6.1527
36. Kenngott RA, Vermehren M, Sauer U, Ebach K, Sinowatz F: Cellular expression and localization of estrogen receptor α and progesterone receptor mRNA in the bovine oviduct combining laser-assisted microdissection, quantitative PCR, and in situ hybridization. J Histochem Cytochem, 59, 312-327, 2011. DOI: 10.1369/0022155410397995
37. Tsai MJ, O’Malley BW: Molecular mechanisms of action of steroid/ thyroid receptor superfamily members. Annu Rev Biochem, 63, 451-486, 1994. DOI: 10.1146/annurev.bi.63.070194.002315
38. Valle GR, Cassali GD, Nogueira JC, Castro AC, Reis AM, Cardoso FM, Figueiredo CB, Nascimento EF: Nuclear estrogen and progesterone receptors in the oviduct of heifers under natural and superovulated estrus cycles. Anim Reprod Sci, 101, 28-37, 2007. DOI: 10.1016/j.anireprosci. 2006.08.026
39. García-Palencia P, Sánchez MA, Nieto A, Vilar MP, González M, Veiga-Lopez A, González-Bulnes A, Flores JM: Sex steroid receptor expression in the oviduct and uterus of sheep with estrus synchronized with progestagen or prostaglandin analogues. Anim Reprod Sci, 97, 25-35, 2007. DOI: 10.1016/j.anireprosci.2006.01.001
40. Tasende C, Meikle A, Rodríguez-Piñón M, Forsberg M, Garófalo EG: Estrogen and progesterone receptor content in the pituitary gland and uterus of progesterone-primed and gonadotropin releasing hormonetreated anestrus ewes. Theriogenology, 57, 1719-1731, 2002. DOI: 10.1016/ s0093-691x(02)00665-9